Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	614,720	A→G	E188E (GAA→GAG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	614,720	0	A	G	100.0%	114.6 / NA	33	E188E (GAA→GAG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/19);  total (14/19)

GCTTTAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACG  >  NC_000913/614584‑614856                                                                                                                                         |                                                                                                                                         gCTTTAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGcc                                                                                                                                       <  1:9974/140‑1 (MQ=255)     tAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGcc                                                                                                                                       >  1:4775/1‑136 (MQ=255)     tAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGcc                                                                                                                                       <  2:4775/136‑1 (MQ=255)            ccGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGa                                                                                                                            >  2:6353/1‑140 (MQ=255)             cGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGAt                                                                                                                           >  2:351061/1‑140 (MQ=255)                ccATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCg                                                                                                                        <  2:687328/140‑1 (MQ=255)                ccATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCg                                                                                                                        <  1:222829/140‑1 (MQ=255)                     aCCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCAtt                                                                                                                   <  2:640685/140‑1 (MQ=255)                     aCCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCAtt                                                                                                                   <  2:599859/140‑1 (MQ=255)                     aCCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCAtt                                                                                                                   <  2:561033/140‑1 (MQ=255)                     aCCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCAtt                                                                                                                   <  2:426736/140‑1 (MQ=255)                     aCCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCAtt                                                                                                                   <  2:215916/140‑1 (MQ=255)                              aTCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGa                                                                                                          >  2:575748/1‑140 (MQ=255)                                           aCTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCaa                                                                                             <  1:147211/140‑1 (MQ=255)                                           aCTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCaa                                                                                             <  1:399572/140‑1 (MQ=255)                                                 caTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTgccg                                                                                       >  2:98954/1‑140 (MQ=255)                                                 caTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTgccg                                                                                       >  1:757561/1‑140 (MQ=255)                                                    ggCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAg                                                                                                      <  2:494560/122‑1 (MQ=255)                                                    ggCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAg                                                                                                      >  1:494560/1‑122 (MQ=255)                                                    ggCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGcgt                                                                                                   <  2:288633/125‑1 (MQ=255)                                                    ggCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGcgt                                                                                                   >  1:288633/1‑125 (MQ=255)                                                              cGAACCAACGCCTGCTTCGCCATTTACGCC‑TTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACt                                                                         <  2:391987/140‑1 (MQ=255)                                                                      cGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCg                                                                  <  1:6353/140‑1 (MQ=255)                                                                        ccTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGc                                                                <  1:398770/140‑1 (MQ=255)                                                                                     ttACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCgggg                                                   >  1:247310/1‑140 (MQ=255)                                                                                             ttCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAgcgc                                           <  1:575748/140‑1 (MQ=255)                                                                                                              agagTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTgc                          >  1:283257/1‑140 (MQ=255)                                                                                                              agagTACCAACAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTgc                          >  2:469798/1‑140 (MQ=255)                                                                                                                 gTACCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                       <  1:132879/140‑1 (MQ=255)                                                                                                                   aCCAGCAATACCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTg                     >  1:132581/1‑140 (MQ=255)                                                                                                                            aCCGCGAAAGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGaa            >  2:409516/1‑140 (MQ=255)                                                                                                                                  aaaGCGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTAcc      >  1:648519/1‑140 (MQ=255)                                                                                                                                      cGAGGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACg  <  2:247310/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCTTTAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACG  >  NC_000913/614584‑614856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑