Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,278	A→G	E131E (GAA→GAG)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,278	0	A	G	100.0%	133.9 / NA	39	E131E (GAA→GAG)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/23);  total (16/23)

CCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGC  >  NC_000913/1098141‑1098402                                                                                                                                          |                                                                                                                             ccACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAgta                                                                                                                            >  1:41645/1‑140 (MQ=255)  cACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAgtat                                                                                                                           >  2:717450/1‑140 (MQ=255)    cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         <  2:528430/140‑1 (MQ=255)    cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         <  1:246303/140‑1 (MQ=255)    cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         >  1:254490/1‑140 (MQ=255)    cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGAGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         >  2:232808/1‑140 (MQ=255)          cGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGa                                                                                                                   <  2:179593/140‑1 (MQ=255)           gCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGaa                                                                                                                  <  1:752429/140‑1 (MQ=255)               ggAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGAtt                                                                                                              >  2:646700/1‑140 (MQ=255)                 aaGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGAtttt                                                                                                            <  2:595015/140‑1 (MQ=255)                     tACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCa                                                                                                        <  2:41645/140‑1 (MQ=255)                          gTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGc                                                                                                   >  2:130138/1‑140 (MQ=255)                               ggCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCAtttt                                                                                              >  1:271602/1‑140 (MQ=255)                               ggCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCAtttt                                                                                              >  2:112126/1‑140 (MQ=255)                                  gAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTggg                                                                                           <  2:271602/140‑1 (MQ=255)                                  gAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTggg                                                                                           <  2:254490/140‑1 (MQ=255)                                   aGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgc                                                                                          <  1:130138/140‑1 (MQ=255)                                    gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         >  1:819231/1‑140 (MQ=255)                                    gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         <  1:717450/140‑1 (MQ=255)                                    gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         >  2:480537/1‑140 (MQ=255)                                     cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTAc                                                                                                          <  2:540656/122‑1 (MQ=255)                                     cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTAc                                                                                                          >  1:540656/1‑122 (MQ=255)                                     cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcgc                                                                                        >  1:533920/1‑140 (MQ=255)                                     cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcgc                                                                                        >  2:623090/1‑140 (MQ=255)                                              gAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACt                                                                               <  1:623090/140‑1 (MQ=255)                                                   tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 >  2:141454/1‑133 (MQ=255)                                                   tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 <  1:19455/133‑1 (MQ=255)                                                   tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 >  2:19455/1‑133 (MQ=255)                                                   tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 <  1:141454/133‑1 (MQ=255)                                                             cagGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGc                                                                <  2:533920/140‑1 (MQ=255)                                                              agGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCt                                                               <  1:696060/140‑1 (MQ=255)                                                                           gTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCaaa                                                  >  2:833435/1‑140 (MQ=255)                                                                           gTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCaaa                                                  >  2:101844/1‑140 (MQ=255)                                                                               cGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTg                                              <  1:232808/140‑1 (MQ=255)                                                                                                 cGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTg                            <  1:846709/140‑1 (MQ=255)                                                                                                          cAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCg                   <  1:833435/140‑1 (MQ=255)                                                                                                                 gTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGt            <  2:819231/140‑1 (MQ=255)                                                                                                                 gTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCCCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGt            <  1:197618/140‑1 (MQ=255)                                                                                                                           gCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  <  1:101844/140‑1 (MQ=255)                                                                                                                                   tGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  >  2:485763/1‑132 (MQ=255)                                                                                                                                   tGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  <  1:485763/132‑1 (MQ=255)                                                                                                                                          |                                                                                                                             CCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGC  >  NC_000913/1098141‑1098402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑