Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,359	A→G	E342G (GAG→GGG)	opgG →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,359	0	A	G	100.0%	131.1 / NA	38	E342G (GAG→GGG)	opgG	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/15);  total (23/15)

CGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGAGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCTT  >  NC_000913/1110227‑1110494                                                                                                                                     |                                                                                                                                        cGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGggggtggggt                                                                                                                                  <  2:837366/140‑1 (MQ=255)      agcagcTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAgg                                                                                                                             <  2:841767/140‑1 (MQ=255)                     gAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCtggg                                                                                                              >  2:795298/1‑139 (MQ=255)                         aCCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAGGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGaa                                                                                                          >  2:617116/1‑140 (MQ=255)                              cAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTcc                                                                                                     <  1:442596/140‑1 (MQ=255)                                   cTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTccaacca                                                                                                <  2:362598/140‑1 (MQ=255)                                    tttGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTTACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTccaaccaa                                                                                               >  1:274812/1‑140 (MQ=255)                                      tGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACg                                                                                             >  1:405174/1‑140 (MQ=255)                                             ttGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:801666/1‑140 (MQ=255)                                             ttGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:33264/1‑140 (MQ=255)                                              tGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAcc                                                                                     <  1:119965/140‑1 (MQ=255)                                                   cgcgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGa                                                                                >  2:40610/1‑140 (MQ=255)                                                   cgcgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGa                                                                                >  1:829460/1‑140 (MQ=255)                                                     cgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGTGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATa                                                                              >  2:803920/1‑140 (MQ=255)                                                       gTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:286367/1‑130 (MQ=255)                                                       gTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      <  2:286367/130‑1 (MQ=255)                                                                              gATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGa                                                     <  1:617116/140‑1 (MQ=255)                                                                                 ctcGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCa                                                  >  1:348105/1‑140 (MQ=255)                                                                                    gatgatCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCt                                               >  2:132012/1‑140 (MQ=255)                                                                                         tCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCgg                                          >  1:252352/1‑140 (MQ=255)                                                                                         tCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCgg                                          >  1:247001/1‑140 (MQ=255)                                                                                           gTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAg                                        <  1:132012/140‑1 (MQ=255)                                                                                            ttACGATCTCCGTCCAAGCGCATGGGTGACTCCGAACGGGGGGAGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       >  2:781405/1‑140 (MQ=255)                                                                                                  tctcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTa                                 >  1:206687/1‑140 (MQ=255)                                                                                                    tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCTACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       <  2:773692/132‑1 (MQ=255)                                                                                                    tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       >  1:773692/1‑132 (MQ=255)                                                                                                    tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               >  1:530882/1‑140 (MQ=255)                                                                                                    tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               >  2:752568/1‑140 (MQ=255)                                                                                                    tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               <  1:547440/140‑1 (MQ=255)                                                                                                         ccAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGAt                          <  2:33264/140‑1 (MQ=255)                                                                                                            aGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGaa                       >  2:608573/1‑140 (MQ=255)                                                                                                             gcgcATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAAc                      <  2:348105/140‑1 (MQ=255)                                                                                                                  tGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTaa                 >  1:354970/1‑140 (MQ=255)                                                                                                                     gTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATa              <  1:839925/140‑1 (MQ=255)                                                                                                                       taCTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTGAAGAGATGAACTTTAAATaca            <  2:274812/139‑1 (MQ=255)                                                                                                                          tCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCa         <  2:354970/140‑1 (MQ=255)                                                                                                                             gAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCa      <  2:829460/140‑1 (MQ=255)                                                                                                                              aaaGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCAc     >  2:254600/1‑140 (MQ=255)                                                                                                                               aaGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCAcc    >  1:354440/1‑140 (MQ=255)                                                                                                                                 ggggggTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCtt  <  2:801666/140‑1 (MQ=255)                                                                                                                                 ggggggTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCtt  <  1:254600/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGAGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCTT  >  NC_000913/1110227‑1110494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑