Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,255	A→G	T473A (ACC→GCC)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,255	0	A	G	100.0%	68.2 / NA	21	T473A (ACC→GCC)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/14);  total (7/14)

AGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTG  >  NC_000913/1129121‑1129391                                                                                                                                       |                                                                                                                                         aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATg                                                                                                                                     >  1:11188/1‑140 (MQ=255) aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATg                                                                                                                                     >  2:273558/1‑140 (MQ=255)  gTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGc                                                                                                                                    >  1:506807/1‑140 (MQ=255)   ttGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGcc                                                                                                                                   <  2:506807/140‑1 (MQ=255)            cAGAAAATCTTCACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGttt                                                                                                                          <  2:604374/140‑1 (MQ=255)                              cAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCggg                                                                                                        >  2:583326/1‑139 (MQ=255)                              cAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGt                                                                                                        >  1:646829/1‑140 (MQ=255)                              cAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGt                                                                                                        <  1:78914/140‑1 (MQ=255)                                aCCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCg                                                                                                      <  2:638882/140‑1 (MQ=255)                                           tGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCgggg                                                                                           >  2:369621/1‑140 (MQ=255)                                             gCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTTGCgggggg                                                                                         >  1:40735/1‑138 (MQ=255)                                                                              gTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTggg                                                        <  2:646829/140‑1 (MQ=255)                                                                                                         ttACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgccg                             <  1:583326/140‑1 (MQ=255)                                                                                                         ttACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgccg                             <  1:846699/140‑1 (MQ=255)                                                                                                         ttACATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgccg                             <  2:11188/140‑1 (MQ=255)                                                                                                           aCATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa                           <  2:810974/140‑1 (MQ=255)                                                                                                           aCATATCATGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa                           <  1:464326/140‑1 (MQ=255)                                                                                                                  aTGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTa                    <  1:369621/140‑1 (MQ=255)                                                                                                                  aTGCCGGAGTGGTCATTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTa                    <  2:246066/140‑1 (MQ=255)                                                                                                                                 ttGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTTCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGc     <  2:223635/140‑1 (MQ=255)                                                                                                                                    ggTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtg  <  2:185331/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTG  >  NC_000913/1129121‑1129391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑