Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,288,004	T→C	E207E (GAA→GAG)	purU ←	formyltetrahydrofolate deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,288,004	0	T	C	100.0%	152.0 / NA	43	E207E (GAA→GAG)	purU	formyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

CGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATACTTCGCCAGCACCA  >  NC_000913/1287868‑1288134                                                                                                                                         |                                                                                                                                   cGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCa                                                                                                                                 <  1:233453/140‑1 (MQ=255) cGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCa                                                                                                                                 <  1:577498/140‑1 (MQ=255)   tCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATa                                                                                                                               <  1:147983/140‑1 (MQ=255)           tGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGt                                                                                                                       >  1:622316/1‑140 (MQ=255)            gcgcgcATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGcc                                                                                                                           <  1:172845/135‑1 (MQ=255)            gcgcgcATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGcc                                                                                                                           >  2:60663/1‑135 (MQ=255)            gcgcgcATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGcc                                                                                                                           >  2:172845/1‑135 (MQ=255)            gcgcgcATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGcc                                                                                                                           <  1:60663/135‑1 (MQ=255)                            cAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCa                                                                                                      >  2:664056/1‑140 (MQ=255)                            cAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCa                                                                                                      >  1:817397/1‑140 (MQ=255)                            cAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCa                                                                                                      >  1:51720/1‑140 (MQ=255)                                  tgtAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAAc                                                                                                >  1:240013/1‑140 (MQ=255)                                       gTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTgg                                                                                           <  2:51720/140‑1 (MQ=255)                                       gTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTgg                                                                                           <  2:223097/140‑1 (MQ=255)                                       gTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTgg                                                                                           <  2:817397/140‑1 (MQ=255)                                         aTGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCa                                                                                         <  2:240013/140‑1 (MQ=255)                                            aTCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGga                                                                                      <  2:22220/140‑1 (MQ=255)                                            aTCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGga                                                                                      >  2:407391/1‑140 (MQ=255)                                            aTCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACA‑CACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGgaa                                                                                     >  1:773839/1‑140 (MQ=255)                                              cGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGgaat                                                                                    <  2:622316/140‑1 (MQ=255)                                              cGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGgaat                                                                                    >  2:604970/1‑140 (MQ=255)                                                      aTAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTg                                                                            >  2:49338/1‑140 (MQ=255)                                                       tAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGa                                                                           <  1:49338/140‑1 (MQ=255)                                                            tCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATAtt                                                                      >  1:400721/1‑140 (MQ=255)                                                              cTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATtga                                                                    <  1:620849/140‑1 (MQ=255)                                                               tGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATGATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATtgat                                                                   >  2:597918/1‑140 (MQ=255)                                                               tGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGTAGGAATGAATGGt                                                                             <  1:625653/130‑1 (MQ=255)                                                               tGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGTAGGAATGAATGGt                                                                             >  2:625653/1‑130 (MQ=255)                                                                  atgatgATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTgatgat                                                                <  1:604970/140‑1 (MQ=255)                                                                           gggCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCgg                                                       >  2:8446/1‑140 (MQ=255)                                                                           gggCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCgg                                                       >  1:556715/1‑140 (MQ=255)                                                                             gCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGa                                                     >  1:94718/1‑140 (MQ=255)                                                                                              cATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTcc                                    >  1:715344/1‑140 (MQ=255)                                                                                                       aGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAAt                           >  2:522931/1‑140 (MQ=255)                                                                                                       aGTGAGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAAt                           >  2:577653/1‑140 (MQ=255)                                                                                                           aGCGGTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATAccc                       >  2:437828/1‑140 (MQ=255)                                                                                                              ggTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCa                    <  1:61494/140‑1 (MQ=255)                                                                                                               gTTGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCat                   >  2:753670/1‑140 (MQ=255)                                                                                                                ttGCGCCAATAATCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCata                  >  2:593942/1‑140 (MQ=255)                                                                                                                  gcgcCAATAATCTTCACA‑CACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATAc               <  2:773839/140‑1 (MQ=255)                                                                                                                        ataatCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATACTTCGc          <  2:262374/140‑1 (MQ=255)                                                                                                                            tCTTCACACCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATACTTCGCCAGc      <  1:753670/140‑1 (MQ=255)                                                                                                                                cacaCCACGCTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATACTTCGCCAGcacca  <  1:577653/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGCGTGCCACAAATTCCGGCGTTAATACCCGCATATACTTCGCCAGCACCA  >  NC_000913/1287868‑1288134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑