Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	131,055	A→G	V69A (GTC→GCC)	yacH ←	DUF3300 domain‑containing protein YacH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	131,055	0	A	G	100.0%	150.0 / NA	43	V69A (GTC→GCC)	yacH	DUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

CGACCATTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTA  >  NC_000913/130922‑131189                                                                                                                                      |                                                                                                                                       cGACCATTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGAcgg                                                                                                                                  >  1:764076/1‑140 (MQ=255)   aCCATTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGAcggcg                                                                                                                                <  1:187577/140‑1 (MQ=255)      aTTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGgt                                                                                                                             <  1:770482/140‑1 (MQ=255)      aTTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGgt                                                                                                                             >  1:579255/1‑140 (MQ=255)       ttGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGgtg                                                                                                                            >  2:302702/1‑140 (MQ=255)           aCTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTAc                                                                                                                        >  1:313939/1‑140 (MQ=255)               cTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGa                                                                                                                    >  2:44303/1‑140 (MQ=255)                     cAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCg                                                                                                              <  1:302702/140‑1 (MQ=255)                        cGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGaa                                                                                                           <  1:124177/140‑1 (MQ=255)                         gTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGaaa                                                                                                          >  1:33704/1‑140 (MQ=255)                                  aTAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTg                                                                                                    <  2:404111/137‑1 (MQ=255)                                  aTAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTg                                                                                                    >  1:404111/1‑137 (MQ=255)                                        tGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACg                                                                                           >  1:264698/1‑140 (MQ=255)                                                tCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACcgcg                                                                                   >  1:685366/1‑140 (MQ=255)                                                 cAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCgg                                                                                  >  1:460720/1‑140 (MQ=255)                                                   gCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAAc                                                                                       <  1:595347/133‑1 (MQ=255)                                                   gCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAAc                                                                                       >  2:595347/1‑133 (MQ=255)                                                                 aGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGc                                                                  >  1:347231/1‑140 (MQ=255)                                                                           gATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTc                                                        <  2:264698/140‑1 (MQ=255)                                                                                  cgcgACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCg                                                 >  1:562945/1‑140 (MQ=255)                                                                                          gcgcGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                         <  2:326185/140‑1 (MQ=255)                                                                                          gcgcGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                         <  2:764076/140‑1 (MQ=255)                                                                                          gcgcGACCCATTGATCTATTTGTGCAATGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                         <  2:349916/140‑1 (MQ=255)                                                                                                        tCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                         >  1:569616/1‑126 (MQ=255)                                                                                                        tCTATTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                         <  2:569616/126‑1 (MQ=255)                                                                                                           aTTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCg                        <  2:685366/140‑1 (MQ=255)                                                                                                           aTTTGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCg                        <  2:347231/140‑1 (MQ=255)                                                                                                            tttGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCgg                       >  1:724949/1‑140 (MQ=255)                                                                                                             ttGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      <  2:562945/140‑1 (MQ=255)                                                                                                             ttGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      <  2:313939/140‑1 (MQ=255)                                                                                                             ttGTGCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      <  2:33704/140‑1 (MQ=255)                                                                                                                 gCAGTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCtttt                  >  1:278345/1‑140 (MQ=255)                                                                                                                    gTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      <  2:239860/133‑1 (MQ=255)                                                                                                                    gTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      >  1:239860/1‑133 (MQ=255)                                                                                                                    gTGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtat               >  1:617710/1‑140 (MQ=255)                                                                                                                     tGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAGCCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      <  2:773385/132‑1 (MQ=255)                                                                                                                     tGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                      >  1:773385/1‑132 (MQ=255)                                                                                                                     tGCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtata              >  1:689565/1‑140 (MQ=255)                                                                                                                      gCTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtatat             >  1:393472/1‑140 (MQ=255)                                                                                                                       cTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtatata            >  1:341175/1‑140 (MQ=255)                                                                                                                       cTGAATGCGGATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtatata            <  2:278345/140‑1 (MQ=255)                                                                                                                               ggATTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCgg    >  2:684372/1‑140 (MQ=255)                                                                                                                                 aTTTGGCGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTa  >  1:772447/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CGACCATTGCACTGCTTGAGCAACGTTTGTCGGATAGGTTGATGCCATCAGCACCTGCGAAAGTAGGGCGTCGGGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTA  >  NC_000913/130922‑131189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑