Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,373	T→C	F39L (TTC→CTC)	tus →	DNA replication terminus site‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,373	0	T	C	100.0%	176.8 / NA	51	F39L (TTC→CTC)	tus	DNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/26);  total (25/26)

AAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCC  >  NC_000913/1684245‑1684507                                                                                                                                 |                                                                                                                                       aaGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCg                                                                                                                             <  2:185055/140‑1 (MQ=255)  aGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCgg                                                                                                                            >  2:555678/1‑140 (MQ=255)  aGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCgg                                                                                                                            >  1:75981/1‑140 (MQ=255)   gTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGa                                                                                                                           <  1:791955/140‑1 (MQ=255)       ttAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTa                                                                                                                       >  1:179042/1‑140 (MQ=255)             ttatGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTaaaaaaa                                                                                                                 >  1:521418/1‑140 (MQ=255)             ttatGGCGAGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTaaaaaaa                                                                                                                 <  2:369881/140‑1 (MQ=255)               atGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTa                                                                                                                       >  2:427742/1‑132 (MQ=255)               atGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTa                                                                                                                       <  1:427742/132‑1 (MQ=255)                 ggCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAgg                                                                                                             <  1:854157/140‑1 (MQ=255)                  gcgcGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGa                                                                                                            <  1:171996/140‑1 (MQ=255)                  gcgcGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGa                                                                                                            <  1:277730/140‑1 (MQ=255)                  gcgcGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGa                                                                                                            <  1:781839/140‑1 (MQ=255)                    gcgTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATg                                                                                                          >  2:360141/1‑140 (MQ=255)                     cgTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGa                                                                                                         >  1:116520/1‑140 (MQ=255)                       ttACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGc                                                                                                       <  1:360141/140‑1 (MQ=255)                                cGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGa                                                                                                            >  1:119843/1‑126 (MQ=255)                                cGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGa                                                                                                            <  2:119843/126‑1 (MQ=255)                                    gACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTaa                                                                                          <  2:371056/140‑1 (MQ=255)                                     aCCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAAt                                                                                         <  2:75981/140‑1 (MQ=255)                                       cGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCg                                                                                       >  1:567717/1‑140 (MQ=255)                                         aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCTTCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGCAAAAAAAGAGGATGAGCATAATCCGCTTaa                                                                                          >  2:780045/1‑135 (MQ=255)                                         aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACTCAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTaa                                                                                          <  1:780045/135‑1 (MQ=255)                                         aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTa                                                                                     >  2:808840/1‑140 (MQ=255)                                            cAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTg                                                                                  <  1:555678/140‑1 (MQ=255)                                                 cTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTa                                                                             >  2:137767/1‑140 (MQ=255)                                                    ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGGGGTaaaa                                                                          >  1:584859/1‑140 (MQ=255)                                                       ttCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAaacaa                                                                       <  2:179042/140‑1 (MQ=255)                                                         cGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTAtt                                                                                   >  1:108785/1‑126 (MQ=255)                                                         cGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTAtt                                                                                   <  2:108785/126‑1 (MQ=255)                                                                             ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                 >  1:649555/1‑140 (MQ=255)                                                                             ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                 >  1:241731/1‑140 (MQ=255)                                                                              gCTATATTTGCCGTTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTc                                                >  1:755284/1‑140 (MQ=255)                                                                                     ttGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCg                                         >  1:88083/1‑140 (MQ=255)                                                                                        ccGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCa                                                   >  1:396830/1‑127 (MQ=255)                                                                                        ccGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCa                                                   <  2:396830/127‑1 (MQ=255)                                                                                        ccGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTg                                      >  1:674037/1‑140 (MQ=255)                                                                                                      cAACACAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                        <  2:88083/140‑1 (MQ=255)                                                                                                        acacAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                        >  1:146019/1‑138 (MQ=255)                                                                                                        acacAAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                        <  2:146019/138‑1 (MQ=255)                                                                                                         cacaAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCattt                     >  2:459629/1‑140 (MQ=255)                                                                                                          acaAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCattta                    >  2:735281/1‑140 (MQ=255)                                                                                                           caAGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttat                   <  2:539784/140‑1 (MQ=255)                                                                                                            aaGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  <  1:31393/140‑1 (MQ=255)                                                                                                            aaGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  <  1:735281/140‑1 (MQ=255)                                                                                                            aaGCTATTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  <  1:459629/140‑1 (MQ=255)                                                                                                                 aTTGGTTGCCCGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTc             <  2:584859/140‑1 (MQ=255)                                                                                                                          ccGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  <  1:148951/126‑1 (MQ=255)                                                                                                                          ccGCGTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  >  2:148951/1‑126 (MQ=255)                                                                                                                            gcgTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCGTTTATTTATTCAACAACAGTcc  >  1:611729/1‑140 (MQ=255)                                                                                                                             cgTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  <  2:241000/123‑1 (MQ=255)                                                                                                                             cgTGCTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                  >  1:241000/1‑123 (MQ=255)                                                                                                                                 |                                                                                                                                       AAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCC  >  NC_000913/1684245‑1684507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑