Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,817,080	T→C	N19S (AAC→AGC)	chbG ←	chitin disaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,817,080	0	T	C	100.0%	151.0 / NA	43	N19S (AAC→AGC)	chbG	chitin disaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

GACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGCCATTTCTCGTGCGCCAGA  >  NC_000913/1816947‑1817214                                                                                                                                      |                                                                                                                                       gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGAc                                                                                                                                  >  2:434070/1‑140 (MQ=255) gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGAc                                                                                                                                  >  2:757761/1‑140 (MQ=255) gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGAc                                                                                                                                  >  2:238844/1‑140 (MQ=255) gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGAc                                                                                                                                  >  2:793442/1‑140 (MQ=255) gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTAACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGAc                                                                                                                                  >  2:405100/1‑140 (MQ=255)     aaGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCttt                                                                                                                              >  1:517668/1‑140 (MQ=255)       gTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGc                                                                                                                            >  2:584291/1‑140 (MQ=255)        tGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCt                                                                                                                           <  2:210749/140‑1 (MQ=255)                 aTGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCaaa                                                                                                                  >  2:415665/1‑140 (MQ=255)                  tGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCaaaa                                                                                                                 >  2:571889/1‑140 (MQ=255)                  tGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCaaaa                                                                                                                 >  1:853348/1‑140 (MQ=255)                     ccAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAatca                                                                                                              <  2:48769/140‑1 (MQ=255)                       aGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTGAGCCAAAatcatc                                                                                                            <  1:757761/140‑1 (MQ=255)                       aGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAatcatc                                                                                                            <  1:238844/140‑1 (MQ=255)                       aGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAatcatc                                                                                                            <  1:434070/140‑1 (MQ=255)                       aGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAatcatc                                                                                                            <  1:793442/140‑1 (MQ=255)                             ggTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCGCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCAtt                                                                                                      <  2:216613/140‑1 (MQ=255)                                    caCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATc                                                                                               <  1:53873/140‑1 (MQ=255)                                            aaCTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCg                                                                                       >  1:334107/1‑140 (MQ=255)                                                   ccGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCg                                                                                       <  1:857687/133‑1 (MQ=255)                                                   ccGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCg                                                                                       >  2:857687/1‑133 (MQ=255)                                                    cGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATaa                                                                               >  2:602965/1‑140 (MQ=255)                                                     gCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAAt                                                                              <  1:571889/140‑1 (MQ=255)                                                      cATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGc                                                                                        >  1:619588/1‑129 (MQ=255)                                                      cATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGc                                                                                        <  2:619588/129‑1 (MQ=255)                                                      cATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATc                                                                             >  1:394467/1‑140 (MQ=255)                                                         ggTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGc                                                                          <  2:334107/140‑1 (MQ=255)                                                         ggTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGc                                                                          <  1:415665/140‑1 (MQ=255)                                                                     gCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAAt                                                                              <  2:630083/124‑1 (MQ=255)                                                                     gCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAAt                                                                              >  1:630083/1‑124 (MQ=255)                                                                     gCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAAtgt                                                              >  2:722258/1‑140 (MQ=255)                                                                     gCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAAtgt                                                              >  1:419487/1‑140 (MQ=255)                                                                     gCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGCTAAtgt                                                              >  1:865963/1‑140 (MQ=255)                                                                                aGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAg                                                   <  2:517668/140‑1 (MQ=255)                                                                                aGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAg                                                   >  1:36227/1‑140 (MQ=255)                                                                                         gtcgACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGAt                                          >  2:750116/1‑140 (MQ=255)                                                                                                     aTCCCATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCaaa                              <  2:36227/140‑1 (MQ=255)                                                                                                         cATTGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGCTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGttt                          <  2:865963/140‑1 (MQ=255)                                                                                                            tGCGACAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGc                       <  1:301784/140‑1 (MQ=255)                                                                                                                aCAGGCCTCGATAATGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGcc                   >  2:297822/1‑140 (MQ=255)                                                                                                                             aTGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGCCATTTCTCGTgcgc      <  1:584291/140‑1 (MQ=255)                                                                                                                              tGCCGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGCCATTTCTCGTGCGcc     >  2:250485/1‑140 (MQ=255)                                                                                                                                 cGTAGCTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGCCATTTCTCGTGCGCCAGa  <  1:595219/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGGCAAAGTTTGGCAGCCATTTCTCGTGCGCCAGA  >  NC_000913/1816947‑1817214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑