Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,433	T→C	V160A (GTT→GCT)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,433	0	T	C	100.0%	160.8 / NA	46	V160A (GTT→GCT)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

ATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCA  >  NC_000913/1869296‑1869568                                                                                                                                          |                                                                                                                                        aTTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTc                                                                                                                                       >  2:557641/1‑140 (MQ=255)    tGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCgg                                                                                                                                    <  1:706982/140‑1 (MQ=255)        tttCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCaa                                                                                                                                <  1:802655/140‑1 (MQ=255)                ttCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAata                                                                                                                              <  2:359382/134‑1 (MQ=255)                ttCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAata                                                                                                                              >  1:359382/1‑134 (MQ=255)                ttCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCg                                                                                                                        >  1:585219/1‑140 (MQ=255)                  cGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGtt                                                                                                                      <  1:180559/140‑1 (MQ=255)                               tCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGc                                                                                                         >  2:584352/1‑140 (MQ=255)                                  tGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGa                                                                                                      >  2:260675/1‑140 (MQ=255)                                     tCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACt                                                                                                   >  2:150753/1‑140 (MQ=255)                                      cTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCAtt                                                                                                           <  2:75759/131‑1 (MQ=255)                                      cTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCAtt                                                                                                           >  1:75759/1‑131 (MQ=255)                                         ctctTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACt                                                                                               >  2:703079/1‑140 (MQ=255)                                         ctctTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACt                                                                                               >  1:173367/1‑140 (MQ=255)                                         ctctTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACt                                                                                               >  2:335348/1‑140 (MQ=255)                                          tctTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGGGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTg                                                                                              <  2:589653/140‑1 (MQ=255)                                          tctTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTg                                                                                              <  1:584352/140‑1 (MQ=255)                                                      tGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGcgcg                                                                                         <  1:351041/133‑1 (MQ=255)                                                      tGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGcgcg                                                                                         >  2:351041/1‑133 (MQ=255)                                                        gCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGc                                                                                >  2:644672/1‑140 (MQ=255)                                                        gCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGc                                                                                >  1:315172/1‑140 (MQ=255)                                                                            aaaCCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAgcggc                                                            >  2:740215/1‑140 (MQ=255)                                                                             aaCCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCACTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAgcggcg                                                           >  1:108709/1‑140 (MQ=255)                                                                              aCCAACAACGCCAGCTGACCGTATATAAAACGCCTCGGGTGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                          <  2:788851/140‑1 (MQ=255)                                                                                      cGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                          >  2:205320/1‑132 (MQ=255)                                                                                      cGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                          <  1:205320/132‑1 (MQ=255)                                                                                       gCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCAt                                                 >  1:226406/1‑140 (MQ=255)                                                                                        ccAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATg                                                >  2:104796/1‑140 (MQ=255)                                                                                          aGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                          >  1:132484/1‑128 (MQ=255)                                                                                          aGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                          <  2:132484/128‑1 (MQ=255)                                                                                             tGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTg                                           <  2:173367/140‑1 (MQ=255)                                                                                              gACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                             >  2:424004/1‑137 (MQ=255)                                                                                              gACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                             <  1:424004/137‑1 (MQ=255)                                                                                                           aCGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAag                                       <  1:689176/130‑1 (MQ=255)                                                                                                           aCGCATCGGGCGGGTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAag                                       >  2:689176/1‑130 (MQ=255)                                                                                                                    gcgggTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAg                    <  2:315172/140‑1 (MQ=255)                                                                                                                     cgggTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGc                   <  1:709734/140‑1 (MQ=255)                                                                                                                     cgggTTATACCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGc                   <  1:260675/140‑1 (MQ=255)                                                                                                                             aCCGCTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGa           <  2:585219/140‑1 (MQ=255)                                                                                                                                 cTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCt       >  1:857684/1‑140 (MQ=255)                                                                                                                                 cTAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCt       >  2:529142/1‑140 (MQ=255)                                                                                                                                  tAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGa           <  1:583040/135‑1 (MQ=255)                                                                                                                                  tAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGa           >  2:583040/1‑135 (MQ=255)                                                                                                                                  tAACGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTg      >  2:541744/1‑140 (MQ=255)                                                                                                                                    aCGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTgcg    >  1:391332/1‑140 (MQ=255)                                                                                                                                     cGGCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTgcgc   <  2:226406/140‑1 (MQ=255)                                                                                                                                      ggCGCTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCa  <  2:397286/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCA  >  NC_000913/1869296‑1869568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑