Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,384	A→G	K448K (AAA→AAG)	dgcJ →	putative diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,384	0	A	G	100.0%	203.4 / NA	59	K448K (AAA→AAG)	dgcJ	putative diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAA  >  NC_000913/1873248‑1873516                                                                                                                                         |                                                                                                                                     cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  1:545977/140‑1 (MQ=255) cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  1:383220/140‑1 (MQ=255)           tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACata                                                                                                                         >  1:807990/1‑140 (MQ=255)           tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACata                                                                                                                         <  1:304260/140‑1 (MQ=255)            cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACatat                                                                                                                        >  1:809236/1‑140 (MQ=255)              ggCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACattcgc                                                                                                                      >  1:688854/1‑136 (MQ=255)              ggCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcg                                                                                                                      >  2:108312/1‑140 (MQ=255)                cGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgcg                                                                                                                    >  1:541546/1‑140 (MQ=255)                 gATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCgcgc                                                                                                                   <  2:692895/140‑1 (MQ=255)                   ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg                                                                                                                 <  2:809236/140‑1 (MQ=255)                   ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg                                                                                                                 <  1:460151/140‑1 (MQ=255)                    tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccgc                                                                                                                >  2:844152/1‑140 (MQ=255)                    tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccgc                                                                                                                <  1:464356/140‑1 (MQ=255)                             gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga                                                                                                       >  2:341665/1‑140 (MQ=255)                             gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga                                                                                                       >  1:780616/1‑140 (MQ=255)                                  gTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGc                                                                                                  <  1:341665/140‑1 (MQ=255)                                    aaaaGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt                                                                                                <  1:844152/140‑1 (MQ=255)                                    aaaaGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACAT‑TCGCGCCGCAGAAAGAGATCGGCTtc                                                                                               <  2:688854/140‑1 (MQ=255)                                     aaaGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTtc                                                                                               >  1:700392/1‑140 (MQ=255)                                            ttATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCg                                                                                        >  2:337241/1‑140 (MQ=255)                                                gCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTAt                                                                                    >  2:815491/1‑140 (MQ=255)                                                  cATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTAttt                                                                                  >  2:705461/1‑140 (MQ=255)                                                   aTCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTa                                                                                 <  2:478780/140‑1 (MQ=255)                                                     ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                               >  2:461231/1‑140 (MQ=255)                                                            ggTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaa                                                                        >  2:76522/1‑140 (MQ=255)                                                             gTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaa                                                                       <  1:772603/140‑1 (MQ=255)                                                              tGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGTAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGGTgaaag                                                                      >  2:156350/1‑140 (MQ=255)                                                                   aTGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAAc                                                                 <  2:556560/140‑1 (MQ=255)                                                                      aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt                                                              <  1:821159/140‑1 (MQ=255)                                                                                catTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatg                                                    >  1:771062/1‑140 (MQ=255)                                                                                 atTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatga                                                   <  1:65819/140‑1 (MQ=255)                                                                                  ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  <  1:815491/140‑1 (MQ=255)                                                                                  ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  <  2:274934/140‑1 (MQ=255)                                                                                  ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  >  1:632016/1‑140 (MQ=255)                                                                                   tCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgatg                                                 >  1:230990/1‑140 (MQ=255)                                                                                     ttGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCg                                               >  1:576096/1‑140 (MQ=255)                                                                                     ttGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCGGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCg                                               >  1:736208/1‑140 (MQ=255)                                                                                            ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAg                                        >  2:726987/1‑140 (MQ=255)                                                                                            ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAg                                        >  1:82704/1‑140 (MQ=255)                                                                                              cGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCt                                      <  2:858716/140‑1 (MQ=255)                                                                                                  gccgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCg                                  <  1:76522/140‑1 (MQ=255)                                                                                                      cAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                              <  1:337241/140‑1 (MQ=255)                                                                                                       aaaTTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAg                             <  2:700392/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  2:576096/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  1:705461/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  2:25518/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  2:230990/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCGGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  2:736208/140‑1 (MQ=255)                                                                                                                    aCTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCa                <  1:473468/140‑1 (MQ=255)                                                                                                                        ccTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                              <  2:379123/122‑1 (MQ=255)                                                                                                                        ccTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                              >  1:379123/1‑122 (MQ=255)                                                                                                                            aaCGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAg        <  2:82704/140‑1 (MQ=255)                                                                                                                             aCGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGa       >  1:525527/1‑140 (MQ=255)                                                                                                                                 aTCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAAt              <  2:238699/129‑1 (MQ=255)                                                                                                                                 aTCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAAt              >  1:238699/1‑129 (MQ=255)                                                                                                                                 aTCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGc          >  2:221925/1‑133 (MQ=255)                                                                                                                                 aTCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGc          <  1:221925/133‑1 (MQ=255)                                                                                                                                  tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg           <  2:532806/131‑1 (MQ=255)                                                                                                                                  tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg           >  1:532806/1‑131 (MQ=255)                                                                                                                                  tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaaa  >  1:516481/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAA  >  NC_000913/1873248‑1873516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑