Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,917,976	T→C	L156P (CTG→CCG)	yebT →	intermembrane transport protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,917,976	0	T	C	100.0%	146.5 / NA	42	L156P (CTG→CCG)	yebT	intermembrane transport protein YebT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/25);  total (17/25)

CAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAG  >  NC_000913/1917847‑1918107                                                                                                                                  |                                                                                                                                    cAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGc                                                                                                                           >  1:563760/1‑140 (MQ=255)  aaaaGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCg                                                                                                                          <  1:73626/140‑1 (MQ=255)         tCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCtgat                                                                                                                   <  2:286090/140‑1 (MQ=255)            ttGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTgatgat                                                                                                                <  1:27578/140‑1 (MQ=255)               gCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCa                                                                                                             <  1:101835/140‑1 (MQ=255)                     gtCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCa                                                                                                       <  1:719164/140‑1 (MQ=255)                                 gACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct                                                                                           >  2:644232/1‑140 (MQ=255)                                  aCGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAg                                                                                                     <  2:816979/129‑1 (MQ=255)                                  aCGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAg                                                                                                     >  1:816979/1‑129 (MQ=255)                                       cTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTc                                                                                     >  1:551224/1‑140 (MQ=255)                                                gggAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAg                                                                            <  1:268332/140‑1 (MQ=255)                                                      tataTCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTc                                                                      <  1:644232/140‑1 (MQ=255)                                                        tatCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCAt                                                                    >  1:867630/1‑140 (MQ=255)                                                         atCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCAtt                                                                   >  1:865733/1‑140 (MQ=255)                                                           cGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTgg                                                                 <  2:489990/140‑1 (MQ=255)                                                            ggCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGt                                                                <  2:229556/140‑1 (MQ=255)                                                             gCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTc                                                               >  1:842518/1‑140 (MQ=255)                                                                tgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAt                                                            >  2:478011/1‑140 (MQ=255)                                                                tgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAt                                                            <  2:867630/140‑1 (MQ=255)                                                                tgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAt                                                            >  1:783294/1‑140 (MQ=255)                                                                tgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAt                                                            <  1:247655/140‑1 (MQ=255)                                                                  atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCAt                                                                    <  1:754687/130‑1 (MQ=255)                                                                  atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCAt                                                                    >  2:754687/1‑130 (MQ=255)                                                                  atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAttt                                                          >  1:86739/1‑140 (MQ=255)                                                                  atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTCATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTAttt                                                          >  1:146370/1‑140 (MQ=255)                                                                   tgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTc                                                         <  2:551224/140‑1 (MQ=255)                                                                             aggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATcc                                               >  1:399746/1‑140 (MQ=255)                                                                                taaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCgg                                            <  2:865733/140‑1 (MQ=255)                                                                                      gCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGaa                                      <  2:842518/140‑1 (MQ=255)                                                                                       cAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCgg                                            <  1:767926/133‑1 (MQ=255)                                                                                       cAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCgg                                            >  2:767926/1‑133 (MQ=255)                                                                                         ggATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAg                                   >  1:124836/1‑140 (MQ=255)                                                                                           aTCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTc                                 <  2:124836/140‑1 (MQ=255)                                                                                               cTTTGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACg                             >  2:584645/1‑140 (MQ=255)                                                                                                tttGTCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGa                            <  2:783294/140‑1 (MQ=255)                                                                                                    tCGCACTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTCATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTAt                        <  2:146370/140‑1 (MQ=255)                                                                                                         cTCGATACCCAACCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCAt                   <  2:563760/140‑1 (MQ=255)                                                                                                                    aCCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCAt                   <  2:750009/129‑1 (MQ=255)                                                                                                                    aCCGAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCAt                   >  1:750009/1‑129 (MQ=255)                                                                                                                       gAAATATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAAcaagc     <  1:294287/140‑1 (MQ=255)                                                                                                                        aaaTATCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCAGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAAcaagca    <  1:59442/140‑1 (MQ=255)                                                                                                                          atatCGGCCGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAAcaagcaag  <  1:584645/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    CAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAG  >  NC_000913/1917847‑1918107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑