Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	161,232	A→G	A85A (GCT→GCC)	sfsA ←	putative DNA‑binding transcriptional regulator of maltose metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	161,232	0	A	G	100.0%	123.1 / NA	35	A85A (GCT→GCC)	sfsA	putative DNA‑binding transcriptional regulator of maltose metabolism
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

TCTGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCG  >  NC_000913/161095‑161361                                                                                                                                          |                                                                                                                                  tCTGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGc                                                                                                                                 >  2:700496/1‑140 (MQ=255) tCTGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGc                                                                                                                                 >  1:679504/1‑140 (MQ=255)   tGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGccc                                                                                                                               >  2:448101/1‑140 (MQ=255)   tGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGccc                                                                                                                               >  1:528816/1‑140 (MQ=255)   tGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGccc                                                                                                                               >  2:373286/1‑140 (MQ=255)   tGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGccc                                                                                                                               >  2:144314/1‑140 (MQ=255)   tGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGccc                                                                                                                               >  1:776567/1‑140 (MQ=255)      aCGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCaaa                                                                                                                            >  1:75107/1‑140 (MQ=255)           aaTCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCgtg                                                                                                                       <  2:6275/140‑1 (MQ=255)           aaTCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCgtg                                                                                                                       >  1:6275/1‑140 (MQ=255)               cGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGa                                                                                                                   <  2:75107/140‑1 (MQ=255)                gCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGAc                                                                                                                  <  2:861639/140‑1 (MQ=255)                      aaCATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCaaat                                                                                                            >  1:145005/1‑140 (MQ=255)                               tCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGcgcc                                                                                                   >  1:841234/1‑140 (MQ=255)                                cAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGcgccg                                                                                                  <  2:841234/140‑1 (MQ=255)                                   tACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGctc                                                                                               >  1:593441/1‑140 (MQ=255)                                   tACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGctc                                                                                               >  1:389793/1‑140 (MQ=255)                                   tACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCCGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGctc                                                                                               >  2:834395/1‑140 (MQ=255)                                              tCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGt                                                                                    <  2:360975/140‑1 (MQ=255)                                              tCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGt                                                                                    <  2:593441/140‑1 (MQ=255)                                                        aTTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAg                                                                          <  1:86146/140‑1 (MQ=255)                                                                      ttCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTAttt                                                            <  1:272635/140‑1 (MQ=255)                                                                               cTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgt                                                   <  1:488998/140‑1 (MQ=255)                                                                                tataGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgtg                                                  <  1:700496/140‑1 (MQ=255)                                                                                tataGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgtg                                                  <  2:145005/140‑1 (MQ=255)                                                                                         aCAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGaa                                         >  1:759471/1‑140 (MQ=255)                                                                                                 gAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATa                                 <  2:528816/140‑1 (MQ=255)                                                                                                      tGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGa                            <  2:576699/140‑1 (MQ=255)                                                                                                      tGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGa                            <  2:679504/140‑1 (MQ=255)                                                                                                                      gCCTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGt            >  2:706281/1‑140 (MQ=255)                                                                                                                       ccTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt           >  2:638148/1‑140 (MQ=255)                                                                                                                       ccTCTTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt           <  2:776567/140‑1 (MQ=255)                                                                                                                        ctctTTCGTCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTg          <  1:577443/140‑1 (MQ=255)                                                                                                                                tCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCg  <  2:10951/140‑1 (MQ=255)                                                                                                                                tCAACCTGTTGGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCg  >  1:10951/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  TCTGGACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCG  >  NC_000913/161095‑161361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑