Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,945,088	A→G	V90A (GTG→GCG)	ruvB ←	Holliday junction branch migration complex subunit RuvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,945,088	0	A	G	100.0%	150.0 / NA	43	V90A (GTG→GCG)	ruvB	Holliday junction branch migration complex subunit RuvB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/14);  total (29/14)

CAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTT  >  NC_000913/1944952‑1945212                                                                                                                                         |                                                                                                                             cAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCg                                                                                                                           >  1:313571/1‑140 (MQ=255)         aGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGaa                                                                                                                   >  1:446993/1‑140 (MQ=255)                         gTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTaa                                                                                                   >  1:148749/1‑140 (MQ=255)                         gTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTaa                                                                                                   >  2:194446/1‑140 (MQ=255)                          tACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAAc                                                                                                  <  2:313571/140‑1 (MQ=255)                           aCAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACg                                                                                                 <  2:446993/140‑1 (MQ=255)                             aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGcc                                                                                               >  2:168975/1‑140 (MQ=255)                               cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAAGAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCa                                                                                             >  2:595583/1‑140 (MQ=255)                                    cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCa                                                                                        >  2:750629/1‑140 (MQ=255)                                    cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCa                                                                                        >  2:745971/1‑140 (MQ=255)                                                          gTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGa                                                                                 <  2:386580/125‑1 (MQ=255)                                                          gTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGa                                                                                 >  1:386580/1‑125 (MQ=255)                                                              aTCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                                                                  >  1:106424/1‑136 (MQ=255)                                                              aTCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                                                                  <  2:106424/136‑1 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       <  1:463219/140‑1 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       <  1:745971/140‑1 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       <  1:765300/140‑1 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       >  1:844429/1‑140 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       >  2:765300/1‑140 (MQ=255)                                                                     caATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                                                       >  2:458064/1‑140 (MQ=255)                                                                             aGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgg                                               <  2:844429/140‑1 (MQ=255)                                                                               cACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag                                             >  2:109922/1‑140 (MQ=255)                                                                                aCGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggagg                                            >  2:865112/1‑140 (MQ=255)                                                                                  gTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGAc                                          >  1:97917/1‑140 (MQ=255)                                                                                  gTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGAc                                          >  1:450619/1‑140 (MQ=255)                                                                                         ggTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAAt                                   <  1:750629/140‑1 (MQ=255)                                                                                             cAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaac                               >  1:687193/1‑140 (MQ=255)                                                                                             cAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaac                               >  1:450058/1‑140 (MQ=255)                                                                                              aaGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaaca                              >  1:650901/1‑140 (MQ=255)                                                                                                     gTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGACGCCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGCAGTTTTACCCAACCCCGGGGCACCAAAAATCAACAAACGacc                       >  2:472383/1‑138 (MQ=255)                                                                                                     gTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc                       <  2:527386/140‑1 (MQ=255)                                                                                                     gTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc                       <  2:97917/140‑1 (MQ=255)                                                                                                     gTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc                       <  2:450058/140‑1 (MQ=255)                                                                                                     gTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc                       >  1:467136/1‑140 (MQ=255)                                                                                                          cATCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAggg                  >  2:265187/1‑140 (MQ=255)                                                                                                            tCGCAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCa                >  1:119412/1‑140 (MQ=255)                                                                                                               cAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaacaa                             >  2:248747/1‑124 (MQ=255)                                                                                                               cAGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaacaa                             <  1:248747/124‑1 (MQ=255)                                                                                                                aGCCAAATCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATcgc            >  1:384436/1‑140 (MQ=255)                                                                                                                    aaaTCGCCCGCCTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCcgcg        <  1:265187/140‑1 (MQ=255)                                                                                                                          cccgccTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGttt  >  2:407107/1‑140 (MQ=255)                                                                                                                          cccgccTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGttt  >  1:58828/1‑140 (MQ=255)                                                                                                                          cccgccTTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGttt  >  1:320815/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             CAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTT  >  NC_000913/1944952‑1945212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑