Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,065,685	A→G	V27A (GTT→GCT)	cobU ←	cobinamide‑P guanylyltransferase/cobinamide kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,065,685	0	A	G	100.0%	234.1 / NA	67	V27A (GTT→GCT)	cobU	cobinamide‑P guanylyltransferase/cobinamide kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/38);  total (29/38)

CTGCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGA  >  NC_000913/2065554‑2065815                                                                                                                                    |                                                                                                                                   cTGCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGaa                                                                                                                            <  1:379/140‑1 (MQ=255)  tGCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGaa                                                                                                                            <  1:32351/139‑1 (MQ=255)  tGCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGaa                                                                                                                            >  2:32351/1‑139 (MQ=255)   gCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAaga                                                                                                                          >  1:801173/1‑140 (MQ=255)      tcaactaattCATCAAGATGTTGCCAGCGCTCCACTGTGCGCAAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTc                                                                                                                       <  2:801173/135‑1 (MQ=255)            aattCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAAt                                                                                                                 >  1:393237/1‑140 (MQ=255)            aattCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGAGAAGAGTCCCCAAt                                                                                                                 >  1:174777/1‑140 (MQ=255)                   caAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGcc                                                                                                          >  1:594410/1‑140 (MQ=255)                    aaGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCct                                                                                                         <  2:268376/140‑1 (MQ=255)                      gATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCC‑GGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTg                                                                                                      <  2:514852/140‑1 (MQ=255)                       aTGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTg                                                                                                      <  2:331096/140‑1 (MQ=255)                           tGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTg                                                                                                  >  1:725025/1‑140 (MQ=255)                             ccAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGc                                                                                                >  2:429440/1‑140 (MQ=255)                             ccAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGc                                                                                                <  2:393237/140‑1 (MQ=255)                             ccAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGAGAAGAGTCCCCAATAAGCGCCTCTGCGTGGc                                                                                                <  2:174777/140‑1 (MQ=255)                               aGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGa                                                                                              >  2:393799/1‑140 (MQ=255)                                           tgCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCACCTCTGCGTGGCGACTCTTCCCGCt                                                                                   >  1:313959/1‑139 (MQ=255)                                           tgCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCACCTCTGCGTGGCGACTCTTCCCGCt                                                                                   <  2:313959/139‑1 (MQ=255)                                                     cTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGcccc                                                                        >  1:487127/1‑140 (MQ=255)                                                     cTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGcccc                                                                        >  2:139608/1‑140 (MQ=255)                                                     cTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGcccc                                                                        >  2:135281/1‑140 (MQ=255)                                                       ccGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGcccccc                                                                      <  2:373894/140‑1 (MQ=255)                                                            cggcCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCAc                                                                 <  2:725025/140‑1 (MQ=255)                                                               ccTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCaa                                                              >  2:594608/1‑140 (MQ=255)                                                               ccTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCaa                                                              <  2:594410/140‑1 (MQ=255)                                                               ccTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCaa                                                              <  2:487127/140‑1 (MQ=255)                                                               ccTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCaa                                                              <  1:409011/140‑1 (MQ=255)                                                                    ccGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAatca                                                         >  2:426638/1‑140 (MQ=255)                                                                     agacagtgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAatcat                                                        <  1:556549/134‑1 (MQ=255)                                                                         gatgTTCTATCCGTGCAGTCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAatcatc                                                       <  2:788205/137‑1 (MQ=255)                                                                         gatgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAatcatc                                                       >  1:788205/1‑137 (MQ=255)                                                                         gatgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATa                                                    <  2:465471/140‑1 (MQ=255)                                                                         gatgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCG‑GGTAGCGATATACAGAGCCTGTGAAGAGACCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATAc                                                   >  1:532485/1‑140 (MQ=255)                                                                           tgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATAcc                                                  <  2:554762/140‑1 (MQ=255)                                                                                tATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTc                                             <  1:135281/140‑1 (MQ=255)                                                                                  tCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCGCCAAAATCATCATACCGACTCCt                                           >  1:184739/1‑140 (MQ=255)                                                                                  tCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCt                                           >  1:548122/1‑140 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAGTAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTcc                                            >  2:591887/1‑135 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTcc                                            <  1:591887/135‑1 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGAtt                                       <  2:548122/140‑1 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGAtt                                       >  1:468163/1‑140 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGAtt                                       >  1:651581/1‑140 (MQ=255)                                                                                      tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGAtt                                       >  1:298913/1‑140 (MQ=255)                                                                                       gCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTc                                      >  2:322245/1‑140 (MQ=255)                                                                                        cAGCCATCTCATCATCAAGGATTTGCG‑GGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCaa                                    <  2:532485/140‑1 (MQ=255)                                                                                         aGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCaa                                    <  2:414090/140‑1 (MQ=255)                                                                                            cATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTc                                             >  1:21155/1‑128 (MQ=255)                                                                                            cATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTc                                             <  2:21155/128‑1 (MQ=255)                                                                                             aTCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCG‑CCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTa                               <  1:393799/140‑1 (MQ=255)                                                                                              tctcATCATCAAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTa                               <  2:468163/140‑1 (MQ=255)                                                                                                tcatcatcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatg                             <  2:403944/140‑1 (MQ=255)                                                                                                tcatcatcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatg                             <  1:429440/140‑1 (MQ=255)                                                                                                tcatcatcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatg                             <  1:594608/140‑1 (MQ=255)                                                                                                 catcatcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatga                            <  2:409870/140‑1 (MQ=255)                                                                                                   tcatcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatgatg                          >  1:822636/1‑140 (MQ=255)                                                                                                     atcaAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGt                        <  2:298913/140‑1 (MQ=255)                                                                                                       caAGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTAt                      <  1:426638/140‑1 (MQ=255)                                                                                                         aGGATTTGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCTAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTc                    >  2:803604/1‑140 (MQ=255)                                                                                                              ttGCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTga               >  1:613523/1‑140 (MQ=255)                                                                                                                gCGAGGTAGCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaac             <  2:651581/140‑1 (MQ=255)                                                                                                                        gCGATATACAGAGCCTGTGAAGAGTCCCCAATAAGTGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAAt     <  1:719371/140‑1 (MQ=255)                                                                                                                           atatACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCGCCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGa  <  2:184739/140‑1 (MQ=255)                                                                                                                           atatACAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGa  <  1:401182/140‑1 (MQ=255)                                                                                                                              taCAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaa              >  2:277404/1‑125 (MQ=255)                                                                                                                              taCAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaa              <  1:277404/125‑1 (MQ=255)                                                                                                                                cAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaacagaaca       >  1:69746/1‑130 (MQ=255)                                                                                                                                cAGAGCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaacagaaca       <  2:69746/130‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   CTGCATGAATTAATTCATCAAGATGTTGCCAGCGCTCCACTGTGCGCCAGTGCTCCGGGCGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGA  >  NC_000913/2065554‑2065815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑