Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,879	G→A	Q340* (CAA→TAA)	wcaM ←	putative colanic acid biosynthesis protein WcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,879	0	G	A	100.0%	147.9 / NA	42	Q340* (CAA→TAA)	wcaM	putative colanic acid biosynthesis protein WcaM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/27);  total (15/27)

GTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACAACCGTAAATAGC  >  NC_000913/2114747‑2115011                                                                                                                                     |                                                                                                                                     gTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCACAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCa                                                                                                                               <  1:257764/140‑1 (MQ=255)   tGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCAtt                                                                                                                             <  1:408288/140‑1 (MQ=255)     cGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCa                                                                                                                           >  1:404413/1‑140 (MQ=255)          tgttgtGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCgg                                                                                                                      <  1:171815/140‑1 (MQ=255)                  aaCTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTg                                                                                                              <  1:71400/140‑1 (MQ=255)                    cTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCg                                                                                                            <  1:414200/140‑1 (MQ=255)                    cTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCg                                                                                                            <  2:404413/140‑1 (MQ=255)                     tCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCgg                                                                                                           <  1:510096/140‑1 (MQ=255)                       cAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTa                                                                                                         <  1:552268/140‑1 (MQ=255)                         gCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACGAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTAAAATATCATTCACCCGGAAATTTTGCGGTATc                                                                                                       >  2:764482/1‑140 (MQ=255)                         gCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATc                                                                                                       <  2:72369/140‑1 (MQ=255)                                 cgcTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGata                                                                                               >  2:632560/1‑140 (MQ=255)                                  gcTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGatat                                                                                              <  2:485556/140‑1 (MQ=255)                                   cTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGTGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATAtt                                                                                             <  1:683745/140‑1 (MQ=255)                                        tctcAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCt                                                                                        <  1:434518/140‑1 (MQ=255)                                        tctcAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCt                                                                                        <  1:98838/140‑1 (MQ=255)                                        tctcAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGAGCAATTTATAAGAAAGATGAGTATTATCCAGTTAAATATCAATCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCt                                                                                        <  2:814804/140‑1 (MQ=255)                                                  agttagCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTAccc                                                                              >  1:720138/1‑140 (MQ=255)                                                       gcgcCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAg                                                                         <  1:764482/140‑1 (MQ=255)                                                       gcgcCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAg                                                                         <  2:694761/140‑1 (MQ=255)                                                        cgcCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGc                                                                        >  2:607733/1‑140 (MQ=255)                                                             caAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGAtt                                                                   >  1:508309/1‑140 (MQ=255)                                                             caAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGAtt                                                                   >  2:264871/1‑140 (MQ=255)                                                                    aaCAGCATTCCCGGCGGAGATCTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATcc                                                            <  1:486495/140‑1 (MQ=255)                                                                      cAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCg                                                          <  1:210236/140‑1 (MQ=255)                                                                       aGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCgg                                                         <  1:102165/140‑1 (MQ=255)                                                                       aGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCgg                                                         <  2:720138/140‑1 (MQ=255)                                                                                       aTTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGgcg                                                       <  1:477432/126‑1 (MQ=255)                                                                                       aTTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGgcg                                                       >  2:477432/1‑126 (MQ=255)                                                                                       aTTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAAt                                               >  2:444242/1‑134 (MQ=255)                                                                                       aTTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAAt                                               <  1:444242/134‑1 (MQ=255)                                                                                       aTTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTc                                         >  1:354109/1‑140 (MQ=255)                                                                                                   cgcAATTTATAAGCAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTatcaat                             >  1:469660/1‑140 (MQ=255)                                                                                                                cAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTca                <  2:469660/140‑1 (MQ=255)                                                                                                                cAAGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTca                <  1:607733/140‑1 (MQ=255)                                                                                                                  aGATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTcaca              >  1:772053/1‑140 (MQ=255)                                                                                                                   gATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACaa             >  1:591470/1‑140 (MQ=255)                                                                                                                   gATGAGTATTATCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACaa             >  2:260751/1‑140 (MQ=255)                                                                                                                         tattatCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACAACCGTaa       <  2:772053/140‑1 (MQ=255)                                                                                                                              tCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTca                >  2:195123/1‑126 (MQ=255)                                                                                                                              tCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTca                <  1:195123/126‑1 (MQ=255)                                                                                                                              tCCAGTTAAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACAACCGTAAATAGc  <  2:144224/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     GTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAATTGTCACAACCGTAAATAGC  >  NC_000913/2114747‑2115011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑