Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,237,246 T→C T170A (ACC→GCC)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,237,2460TC100.0% 157.3 / NA 45T170A (ACC→GCC) mglCD‑galactose/methyl‑galactoside ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/24);  total (21/24)

ACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGA  >  NC_000913/2237109‑2237382
                                                                                                                                         |                                                                                                                                        
aCCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCag                                                                                                                                        >  2:279415/1‑140 (MQ=255)
 ccGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCaga                                                                                                                                       <  1:66683/140‑1 (MQ=255)
  cGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCagag                                                                                                                                      <  2:842714/140‑1 (MQ=255)
        tGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACACGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCa                                                                                                                                <  2:193397/140‑1 (MQ=255)
         ggCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCAc                                                                                                                               <  1:804143/140‑1 (MQ=255)
                        aCCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGaaa                                                                                                                >  1:445217/1‑140 (MQ=255)
                             aGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGc                                                                                                           >  2:87733/1‑140 (MQ=255)
                             aGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGCGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGc                                                                                                           >  1:179349/1‑140 (MQ=255)
                                gggTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGAc                                                                                                        >  1:132063/1‑140 (MQ=255)
                                        ttCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGAc                                                                                                <  1:496264/140‑1 (MQ=255)
                                        ttCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGAc                                                                                                <  1:518355/140‑1 (MQ=255)
                                        ttCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGAc                                                                                                <  2:132063/140‑1 (MQ=255)
                                          ccACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACaa                                                                                              <  1:419239/140‑1 (MQ=255)
                                          ccACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACaa                                                                                              <  1:217954/140‑1 (MQ=255)
                                          ccACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACaa                                                                                              <  1:598877/140‑1 (MQ=255)
                                            acaacaCCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAg                                                                                            <  2:690213/140‑1 (MQ=255)
                                            acaacaCCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAg                                                                                            >  1:690213/1‑140 (MQ=255)
                                                       aCGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATagag                                                                                 <  2:445217/140‑1 (MQ=255)
                                                        cGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGc                                                                                >  1:821116/1‑140 (MQ=255)
                                                            cGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGt                                                                            >  2:681374/1‑140 (MQ=255)
                                                              ccACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTg                                                                          <  1:279415/140‑1 (MQ=255)
                                                               cACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGa                                                                         >  2:9103/1‑140 (MQ=255)
                                                                    cAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCa                                                                    >  1:587790/1‑140 (MQ=255)
                                                                          aCGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACg                                                              <  2:611180/140‑1 (MQ=255)
                                                                           cgcgTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGa                                                                >  1:795116/1‑137 (MQ=255)
                                                                           cgcgTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGa                                                                <  2:795116/137‑1 (MQ=255)
                                                                                    ggTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCg                                                    >  1:279231/1‑140 (MQ=255)
                                                                                        aTGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGcc                                                <  1:87733/140‑1 (MQ=255)
                                                                                          gTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGa                                              <  1:310273/140‑1 (MQ=255)
                                                                                            aaGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGcc                                                <  1:140639/136‑1 (MQ=255)
                                                                                            aaGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGcc                                                >  2:140639/1‑136 (MQ=255)
                                                                                             agagagACGGAAACTCCCCAGCGCGACGAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCg                                           <  1:300482/140‑1 (MQ=255)
                                                                                                 agaCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGt                                       >  2:136819/1‑140 (MQ=255)
                                                                                                  gaCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGa                                              >  1:493629/1‑132 (MQ=255)
                                                                                                  gaCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGa                                              <  2:493629/132‑1 (MQ=255)
                                                                                                   aCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTaa                                     >  2:738096/1‑140 (MQ=255)
                                                                                                              ccAGCGCGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTc                          <  1:136819/140‑1 (MQ=255)
                                                                                                                 gcgcGACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACg                       >  1:233827/1‑140 (MQ=255)
                                                                                                                    cgACAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTg                    <  1:9103/140‑1 (MQ=255)
                                                                                                                       cAAAGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTgaga                 >  2:44710/1‑140 (MQ=255)
                                                                                                                        aaaGCCCTGAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGAt                <  2:279231/140‑1 (MQ=255)
                                                                                                                                gAGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATa        >  2:229702/1‑140 (MQ=255)
                                                                                                                                 aGCAAAGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATaa       >  1:730163/1‑140 (MQ=255)
                                                                                                                                    aaaGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCa    <  2:821116/140‑1 (MQ=255)
                                                                                                                                      aGGCAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGa  >  2:536311/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGA  >  NC_000913/2237109‑2237382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑