Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,694	T→C	D576G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,694	0	T	C	94.4%	51.5 / ‑5.2	18	D576G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (15/2);  total (16/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAG  >  NC_000913/2337558‑2337824                                                                                                                                         |                                                                                                                                   gTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGcctt                                                                                                                                 <  2:137452/140‑1 (MQ=255) gTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGcctt                                                                                                                                 <  1:821410/140‑1 (MQ=255)         tCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTa                                                                                                                         >  1:286706/1‑140 (MQ=255)          cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTaa                                                                                                                        >  1:841971/1‑140 (MQ=255)             acgacCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGcctt                                                                                                                                 <  2:50861/128‑1 (MQ=255)             acgacCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGcctt                                                                                                                                 >  1:50861/1‑128 (MQ=255)               gacCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGt                                                                                                                   >  2:158449/1‑140 (MQ=255)                    cgcgcgcCACGAGTGGCTTCCGGCAACTGATAAGCTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGc                                                                                                              >  1:664247/1‑140 (MQ=255)                    cgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGc                                                                                                              >  1:812286/1‑140 (MQ=255)                      cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAg                                                                                                            >  1:210811/1‑140 (MQ=255)                                   gCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCgg                                                                                                               <  2:334413/124‑1 (MQ=255)                                   gCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCgg                                                                                                               >  1:334413/1‑124 (MQ=255)                                   gCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTccc                                                                                               >  1:753237/1‑140 (MQ=255)                                              cTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCt                                                                                    >  2:560901/1‑140 (MQ=255)                                                                         aCCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGAt                                                         >  1:748384/1‑140 (MQ=255)                                                                                     aaaGCACAGAATAGGTTCGGGAGTGTCCCCCCCCAGCGGCGGGTAAAAAAACCCTTCTTTTTTAATCCGGGGGGCATTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGc                                             <  2:210811/139‑1 (MQ=255)                                                                                        gCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCt                                          >  2:328841/1‑140 (MQ=255)                                                                                                      cGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTc                            <  1:328841/140‑1 (MQ=255)                                                                                                        tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                          >  2:405865/1‑140 (MQ=255)                                                                                                          aGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGa                        >  1:265472/1‑140 (MQ=255)                                                                                                              ttCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                    >  1:222043/1‑140 (MQ=255)                                                                                                                   caccaGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt               >  1:171858/1‑140 (MQ=255)                                                                                                                      cagcagTAGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt            >  1:49125/1‑140 (MQ=255)                                                                                                                        gcagTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTgg          <  1:158449/140‑1 (MQ=255)                                                                                                                         cagTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg         <  2:265472/140‑1 (MQ=255)                                                                                                                                tcgAAAAACCCTTCTTTTTTAATGGGGGGGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg  <  2:748384/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAG  >  NC_000913/2337558‑2337824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑