Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,403,371	T→C	T159A (ACC→GCC)	nuoC ←	NADH:quinone oxidoreductase subunit CD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,403,371	0	T	C	100.0%	109.4 / NA	32	T159A (ACC→GCC)	nuoC	NADH:quinone oxidoreductase subunit CD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CGCTTCATCCCCCACTCTTCCGGTTTGAAGGTCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGTTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAGGTCGGTACGTGCAGGTCGTTTTC  >  NC_000913/2403235‑2403503                                                                                                                                         |                                                                                                                                     cgcTTCATCCCCCACTCTTCCGGTTTGAAGGTCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTg                                                                                                                                   >  2:744663/1‑140 (MQ=255)        tCCCCCACTCTTCCGGTTTGAAGGTCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGcatc                                                                                                                            >  1:840566/1‑140 (MQ=255)                            aaGGTCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCgg                                                                                                        >  2:303448/1‑140 (MQ=255)                                tCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGa                                                                                                    <  1:522699/140‑1 (MQ=255)                                                   aTCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCaa                                                                                 >  1:200999/1‑140 (MQ=255)                                                   aTCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCACGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCaa                                                                                 >  2:525646/1‑140 (MQ=255)                                                     ccTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAAc                                                                               <  2:840566/140‑1 (MQ=255)                                                        gTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGa                                                                            >  1:463585/1‑140 (MQ=255)                                                          ttGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATc                                                                          >  2:382441/1‑140 (MQ=255)                                                          ttGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATc                                                                          >  2:625954/1‑140 (MQ=255)                                                          ttGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATc                                                                          >  1:575392/1‑140 (MQ=255)                                                             gCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCa                                                                       >  1:261818/1‑140 (MQ=255)                                                             gCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTAGAAAGTAATGCCAAACAGATCCCa                                                                       <  1:802328/140‑1 (MQ=255)                                                                  ggTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGttt                                                                  <  2:826974/140‑1 (MQ=255)                                                                  ggTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGttt                                                                  <  2:261818/140‑1 (MQ=255)                                                                     cAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCAc                                                               <  1:744663/140‑1 (MQ=255)                                                                       gCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGc                                                             <  1:382441/140‑1 (MQ=255)                                                                         tCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTc                                                           <  1:525646/140‑1 (MQ=255)                                                                             aCGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATAc                                                       >  1:245483/1‑140 (MQ=255)                                                                                 cGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGt                                                   <  2:463585/140‑1 (MQ=255)                                                                                              gcgcgcgcCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGaa                                      <  1:384543/140‑1 (MQ=255)                                                                                                 cgcgcCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGaa                                      >  2:812338/1‑137 (MQ=255)                                                                                                 cgcgcCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGaa                                      <  1:812338/137‑1 (MQ=255)                                                                                                  gcgcCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGt                                  <  2:245483/140‑1 (MQ=255)                                                                                                      cGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTg                               >  1:769435/1‑139 (MQ=255)                                                                                                      cGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTg                               <  2:769435/139‑1 (MQ=255)                                                                                                       ggATAATCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGt                             <  1:347161/140‑1 (MQ=255)                                                                                                            aTCTTTACGCAGCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAgg                        <  1:285830/140‑1 (MQ=255)                                                                                                                       gCGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAGGTCGGTACGTGc             <  2:396686/140‑1 (MQ=255)                                                                                                                        cGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAGGTCGGTACGTGCa            >  1:268149/1‑140 (MQ=255)                                                                                                                        cGGGTGACCTTTCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAGGTCGGTACGTGCa            >  1:245211/1‑140 (MQ=255)                                                                                                                                  ttCCAGGCTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGGGAAGGTCGGTACGTGCAGGTCGttttt  >  2:119316/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCTTCATCCCCCACTCTTCCGGTTTGAAGGTCAGGGCTTCCATCTCCAGATCCTGTTTGGCTTTGGTCAGCTCAAACGGCGAGAATTCGGTAGCGCGCGCCGGATAATCTTTACGCAGCGGGTGACCTTTCCAGGTTTGCGGCATCATGATGCGTCGCAGGTTCGGGTGACCGTCGAAAGTAATGCCAAACAGATCCCAGGTTTCACGCTCATACCAGTTAGCGTTCGGGAACAGTTTGGTGAAGGTCGGTACGTGCAGGTCGTTTTC  >  NC_000913/2403235‑2403503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑