Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,412,094	G→A	intergenic (‑5/+6)	hxpA ← / ← yfbU	hexitol phosphatase A/UPF0304 family protein YfbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,412,094	0	G	A	100.0%	108.2 / NA	32	intergenic (‑5/+6)	hxpA/yfbU	hexitol phosphatase A/UPF0304 family protein YfbU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

CGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGGCCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGATATTTTTCCCACATTGGCGTCTGGGCGTTAAAACCGTGAGTT  >  NC_000913/2411957‑2412226                                                                                                                                          |                                                                                                                                     cGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGAcc                                                                                                                                    >  2:316563/1‑140 (MQ=255)      atgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGAcc                                                                                                                                    >  2:689667/1‑135 (MQ=255)      atgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGAcc                                                                                                                                    <  1:689667/135‑1 (MQ=255)      atgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACctcctc                                                                                                                               >  1:839634/1‑140 (MQ=255)         aaaGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAgg                                                                                                                            <  2:698064/140‑1 (MQ=255)         aaaGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAgg                                                                                                                            <  1:316563/140‑1 (MQ=255)                 cACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATa                                                                                                                    >  1:569748/1‑140 (MQ=255)                 cACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATa                                                                                                                    <  1:42436/140‑1 (MQ=255)                         ccGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGAtt                                                                                                            <  1:482913/140‑1 (MQ=255)                                 aaCCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGt                                                                                                    <  2:839634/140‑1 (MQ=255)                                       tGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGgcgc                                                                                              >  1:393443/1‑140 (MQ=255)                                       tGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGgcgc                                                                                              >  2:378678/1‑140 (MQ=255)                                            tCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCaaa                                                                                         <  1:128851/140‑1 (MQ=255)                                              tGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATg                                                                                       <  2:569748/140‑1 (MQ=255)                                              tGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATg                                                                                       >  1:182678/1‑140 (MQ=255)                                                      ttGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGAc                                                                               >  2:11707/1‑140 (MQ=255)                                                       tGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGAcg                                                                              <  1:11707/140‑1 (MQ=255)                                                       tGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGAcg                                                                              <  2:393443/140‑1 (MQ=255)                                                                    ttCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGc                                                                 >  2:79272/1‑140 (MQ=255)                                                                     tCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCATGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGcc                                                                <  2:774070/140‑1 (MQ=255)                                                                      cTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCca                                                               <  2:182678/140‑1 (MQ=255)                                                                      cTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCca                                                               <  1:24067/140‑1 (MQ=255)                                                                      cTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCca                                                               <  2:440817/140‑1 (MQ=255)                                                                      cTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCca                                                               <  1:165774/140‑1 (MQ=255)                                                                               gcaggGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGggcaggc                                                                     >  2:351870/1‑125 (MQ=255)                                                                               gcaggGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGggcaggc                                                                     <  1:351870/125‑1 (MQ=255)                                                                                 aggGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCa                                                    >  1:26749/1‑140 (MQ=255)                                                                                   ggAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTGAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATg                                                  >  2:772287/1‑140 (MQ=255)                                                                                          accaGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGat                                           >  1:75315/1‑140 (MQ=255)                                                                                                  tCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGATATTTTTcc                                   <  2:75315/140‑1 (MQ=255)                                                                                                            tCAAACAGAAAACCTTTGCACCGAACGCGGACTCCCTCAGGAATTGATAATTTAATTAATTTCGTTGGCGCTCAAATGGAACTGACGCGGGCAGGCATGCACCACATTAAGCATGCGCTGATATTTTTCCCACATTGCCg                         >  2:555133/1‑140 (MQ=255)                                                                                                                    aaaaCCTTTGCACCGCACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGATATTTTTCCCACATTGGCGTCTGGGCg                 >  1:791227/1‑140 (MQ=255)                                                                                                                                   cACGCGGACCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGATATTTTTCCCACATTGGCGTCTGGGCGTTAAAACCGTGAGtt  >  1:584862/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGGCCTCCTCAGGCATTGATAATTTGATTAATTTCGTTGGCGCTCAAATGGTACTGACGCGGGCAGGCATGCCACACATTAAGCATGCGCTGATATTTTTCCCACATTGGCGTCTGGGCGTTAAAACCGTGAGTT  >  NC_000913/2411957‑2412226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑