Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,492,953	G→C	A101G (GCC→GGC)	frc ←	formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,492,953	0	G	C	100.0%	172.0 / NA	50	A101G (GCC→GGC)	frc	formyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/25);  total (25/25)

CGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATC  >  NC_000913/2492817‑2493089                                                                                                                                         |                                                                                                                                         cGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGcccc                                                                                                                                       <  2:16205/140‑1 (MQ=255)  gCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCcccc                                                                                                                                      <  2:392717/140‑1 (MQ=255)                          gCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGat                                                                                                              >  1:573692/1‑140 (MQ=255)                                    tCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTcg                                                                                                    <  2:688048/140‑1 (MQ=255)                                     cacaTAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTcgc                                                                                                   >  2:437535/1‑140 (MQ=255)                                         tAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGAt                                                                                               <  1:437535/140‑1 (MQ=255)                                                aaCACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCttt                                                                                        >  1:280217/1‑140 (MQ=255)                                                aaCACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCttt                                                                                        >  2:617007/1‑140 (MQ=255)                                                  cacTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTc                                                                                      >  2:513804/1‑140 (MQ=255)                                                  cacTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTc                                                                                      >  2:522651/1‑140 (MQ=255)                                                      catcaAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCAtt                                                                                  <  1:748008/140‑1 (MQ=255)                                                      catcaAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCAt                                                                                   <  2:700998/139‑1 (MQ=255)                                                      catcaAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCAt                                                                                   >  1:700998/1‑139 (MQ=255)                                                       atcaAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTa                                                                                 >  2:590199/1‑140 (MQ=255)                                                            aCCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACctct                                                                            >  1:383991/1‑140 (MQ=255)                                                               cTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTg                                                                         >  1:782460/1‑140 (MQ=255)                                                                 ttGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTcc                                                                                     >  1:10325/1‑126 (MQ=255)                                                                 ttGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTcc                                                                                     <  2:10325/126‑1 (MQ=255)                                                                 ttGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGcc                                                                       >  2:564374/1‑140 (MQ=255)                                                                 ttGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGcc                                                                       >  2:512878/1‑140 (MQ=255)                                                                      cGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCg                                                                  <  1:617007/140‑1 (MQ=255)                                                                        aaCCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCt                                                                >  1:700616/1‑140 (MQ=255)                                                                        aaCCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCt                                                                >  2:580352/1‑140 (MQ=255)                                                                        aaCCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCt                                                                >  1:352672/1‑140 (MQ=255)                                                                            aaaaaTCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGttt                                                            <  1:513804/140‑1 (MQ=255)                                                                            aaaaaTCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGttt                                                            <  2:782460/140‑1 (MQ=255)                                                                                   aGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTAtt                                                     <  1:532529/140‑1 (MQ=255)                                                                                   aGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTAtt                                                     <  2:280217/140‑1 (MQ=255)                                                                                   aGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTAtt                                                     <  2:700616/140‑1 (MQ=255)                                                                                      cGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTaa                                                  <  2:182485/140‑1 (MQ=255)                                                                                              gattTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAg                                          <  2:137913/140‑1 (MQ=255)                                                                                               attTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGa                                         <  1:522651/140‑1 (MQ=255)                                                                                                    ttGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGtt                                    >  1:450678/1‑140 (MQ=255)                                                                                                    ttGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGtt                                    <  1:152393/140‑1 (MQ=255)                                                                                                     tGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATGTCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGttt                                   >  2:761174/1‑140 (MQ=255)                                                                                                       aaTATGCTCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGt                                 <  2:450678/140‑1 (MQ=255)                                                                                                              tCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGtt                          <  2:383991/140‑1 (MQ=255)                                                                                                              tCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGtt                          >  2:495975/1‑140 (MQ=255)                                                                                                              tCCCAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGtt                          <  1:512878/140‑1 (MQ=255)                                                                                                                ccAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTaa                        <  1:761174/140‑1 (MQ=255)                                                                                                                 cAGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAg                       >  1:145111/1‑140 (MQ=255)                                                                                                                  aGGTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGc                      <  1:529409/140‑1 (MQ=255)                                                                                                                   ggTGAAGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCa                     <  1:759350/140‑1 (MQ=255)                                                                                                                       aaGCCCATGTGATCAATGCTCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGt                                 <  2:596698/124‑1 (MQ=255)                                                                                                                       aaGCCCATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGt                                 >  1:596698/1‑124 (MQ=255)                                                                                                                          cccATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGaa              >  1:307756/1‑140 (MQ=255)                                                                                                                          cccATGTGATCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGaa              >  2:818074/1‑140 (MQ=255)                                                                                                                              tgtgCTCAATGCCCCCAGGCTGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTaa          >  2:58531/1‑140 (MQ=255)                                                                                                                                  aTCAATGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAgcg      >  1:371755/1‑140 (MQ=255)                                                                                                                                     aaTGCCCCCTGGATGAGAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCAt   >  2:673530/1‑140 (MQ=255)                                                                                                                                     aaTGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCAt   <  1:818074/140‑1 (MQ=255)                                                                                                                                      aTGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAg        <  1:190298/134‑1 (MQ=255)                                                                                                                                      aTGCCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATc  <  2:352672/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCTTCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATC  >  NC_000913/2492817‑2493089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑