Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,729,374	T→C	noncoding (71/76 nt)	gltW ←	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,729,374	0	T	C	96.2%	78.1 / ‑4.6	26	noncoding (71/76 nt)	gltW	tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (15/10);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTAT  >  NC_000913/2729242‑2729459                                                                                                                                     |                                                                                      acTTTCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAg                                                                                >  1:440380/1‑140 (MQ=255)  cTTTCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAgg                                                                               >  1:1745/1‑140 (MQ=255)  cTTTCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAgg                                                                               >  2:108186/1‑140 (MQ=255)    ttCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAgggg                                                                             >  1:819717/1‑140 (MQ=255)          gTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTACGGGATTCGa                                                                       >  1:738622/1‑140 (MQ=255)           ttCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGaa                                                                      >  1:341373/1‑140 (MQ=255)            tCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAAc                                                                     >  1:818569/1‑140 (MQ=255)            tCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAAc                                                                     >  1:418260/1‑140 (MQ=255)             cAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGaaac                                                                    >  1:710986/1‑138 (MQ=255)             cAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGaa                                                                      <  1:449557/138‑1 (MQ=255)             cAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGaa                                                                      >  2:449557/1‑138 (MQ=255)             cAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAAcc                                                                    >  2:709498/1‑140 (MQ=255)             cAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAAcc                                                                    <  1:709498/140‑1 (MQ=255)                  tttCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGa                                                                       >  1:59823/1‑132 (MQ=255)                  tttCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGa                                                                       <  2:59823/132‑1 (MQ=255)                          ttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAACCCCTGTTAccgccg                                                       <  1:691935/140‑1 (MQ=255)                             aGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGa                                                    <  2:341373/140‑1 (MQ=255)                               cTTGATCCAGATTTTAAAAGAGAAAATATATCAAACAAAATTTAACATTCTGTTTTGAGATATGGAGGTCGGCGATTTCCCCCCACAACCCAGCAAGTGGCCCCCCCTAGGGGATTCGACCCCCTGTTACCGCCGTGaaa                                                  <  2:418260/140‑1 (MQ=17)                                   aTCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGc                                              >  2:257130/1‑140 (MQ=255)                                   aTCCAGATTTTAAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGATTTTCACTCACAAACCAGCAAGGGGCGCCCCCTAGGGGATTCGACCCCCTGTTACCGCCGTGAAAGGGc                                              <  1:317878/140‑1 (MQ=18)                                            tttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCCCTCACAAACCAGCAAGGGGCGCCCCCTAGGGGATTCGACCCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTg                                     <  1:106678/140‑1 (MQ=255)                                            ttaaaaGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGACCCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTg                                     <  1:563862/137‑1 (MQ=255)                                             ttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTgg                                    >  2:718217/1‑140 (MQ=255)                                              tAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGTATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTggg                                   >  2:808115/1‑140 (MQ=255)                                               aaaGAGAAAATATACCAAACAAAACTTAACACTCTTTTTTGGGATATTGAGGTCGGCTACTTTCCCTCCCAACCCGGCAGGTGGCGCCCCTTGGGGGATTCGACCCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGc                                  <  2:819717/140‑1 (MQ=17)                                                                aaCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGac                 >  1:420163/1‑140 (MQ=255)                                                                             aGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGCCCCCTAGGGGATTCGACCCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCtt    <  2:1745/140‑1 (MQ=255)                                                                               tCTGTTTTGAGATATTGAGGTCGGCAACTTTCACCCACAAACCAGAAAGTGGCGCCCCCTGGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTAt  <  2:710986/140‑1 (MQ=11)                                                                                                                                     |                                                                                      ACTTTCATTGTTCAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTAT  >  NC_000913/2729242‑2729459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑