Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,452	G→A	R759C (CGC→TGC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,452	0	G	A	100.0%	154.7 / NA	45	R759C (CGC→TGC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/19);  total (26/19)

CTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCG  >  NC_000913/2780321‑2780588                                                                                                                                    |                                                                                                                                         ctcCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCtg                                                                                                                                  >  2:480434/1‑140 (MQ=255)          gCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCcgcg                                                                                                                         >  2:32046/1‑140 (MQ=255)          gCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCcgcg                                                                                                                         <  1:32046/140‑1 (MQ=255)          gCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCcgcg                                                                                                                         <  1:480434/140‑1 (MQ=255)           cccATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGt                                                                                                                        <  2:248868/140‑1 (MQ=255)           cccATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGt                                                                                                                        >  1:321765/1‑140 (MQ=255)               tatTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGcc                                                                                                                    <  1:323236/140‑1 (MQ=255)                      cTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCtggtgg                                                                                                             >  1:629104/1‑140 (MQ=255)                      cTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCtggtgg                                                                                                             >  1:591483/1‑140 (MQ=255)                        gtgtTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGtt                                                                                                           >  2:430982/1‑140 (MQ=255)                         tgtTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTg                                                                                                          >  1:717869/1‑140 (MQ=255)                         tgtTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTg                                                                                                          >  1:509887/1‑140 (MQ=255)                           ttttGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCa                                                                                                        >  1:241517/1‑140 (MQ=255)                             ttGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAAc                                                                                                      <  2:629104/140‑1 (MQ=255)                                  agaagaGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAg                                                                                                 >  2:196105/1‑140 (MQ=255)                                   gaagaGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAgc                                                                                                >  1:506928/1‑140 (MQ=255)                                        ggTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCt                                                                                           <  2:500646/140‑1 (MQ=255)                                          tgtCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCt                                                                                           >  1:741202/1‑138 (MQ=255)                                          tgtCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCt                                                                                           <  2:741202/138‑1 (MQ=255)                                          tgtCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTgg                                                                                         <  2:509887/140‑1 (MQ=255)                                            tCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCt                                                                                       >  1:142572/1‑140 (MQ=255)                                              tGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCCCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTAc                                                                                     >  1:515425/1‑140 (MQ=255)                                                aCTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTActc                                                                                   <  2:506928/140‑1 (MQ=255)                                                                gccgccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTg                                                                   <  2:142572/140‑1 (MQ=255)                                                                 ccgccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTgc                                                                  >  2:545111/1‑140 (MQ=255)                                                                  cgccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCCGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTgcg                                                                 >  1:178634/1‑140 (MQ=255)                                                                   gccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTgcgc                                                                <  1:110306/140‑1 (MQ=255)                                                                   gccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTgcgc                                                                <  2:177904/140‑1 (MQ=255)                                                                   gccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTgcgc                                                                >  1:553531/1‑140 (MQ=255)                                                                        ttGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCt                                                           <  2:241517/140‑1 (MQ=255)                                                                                         ttCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAg                                          >  2:641151/1‑140 (MQ=255)                                                                                            gccgcACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAgtgt                                       >  2:166374/1‑140 (MQ=255)                                                                                            gccgcACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAgtgt                                       >  1:128613/1‑140 (MQ=255)                                                                                                 aCCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccgc                                  >  1:105114/1‑140 (MQ=255)                                                                                                 aCCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccgc                                  <  2:553531/140‑1 (MQ=255)                                                                                                 aCCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccgc                                  <  1:430982/140‑1 (MQ=255)                                                                                                 aCCCTGCTCGATGCTGAACGCGACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccgc                                  <  2:321765/140‑1 (MQ=255)                                                                                                                 aacgcaacgcCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccg                                   >  1:518186/1‑123 (MQ=255)                                                                                                                 aacgcaacgcCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccg                                   <  2:518186/123‑1 (MQ=255)                                                                                                                      gACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGtttt             >  1:523856/1‑140 (MQ=255)                                                                                                                      gACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGtttt             >  1:131581/1‑140 (MQ=255)                                                                                                                      gACGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGtttt             >  2:318745/1‑140 (MQ=255)                                                                                                                        cGCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTGTCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCa           >  1:609398/1‑140 (MQ=255)                                                                                                                         gCCATCCGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAg          <  2:523856/140‑1 (MQ=255)                                                                                                                              ccGCGCATGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAgg     <  2:105114/140‑1 (MQ=255)                                                                                                                                 cgcaTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGgccg  <  2:515425/140‑1 (MQ=255)                                                                                                                                 cgcaTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGgccg  <  2:717869/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCG  >  NC_000913/2780321‑2780588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑