| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,833,153 | C→T | S226S (TCC→TCT) | norV → | anaerobic nitric oxide reductase flavorubredoxin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,833,153 | 0 | C | T | 94.1% | 102.3 / ‑1.5 | 34 | S226S (TCC→TCT) | norV | anaerobic nitric oxide reductase flavorubredoxin |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (13/19); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
AGTGGATCAGACGGAGCTTTTCGAGCAGTGCCAGCGTTACTACGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCCCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCGAATAACACCCGCATGATGGCTGACGCTA > NC_000913/2833018‑2833289 | aGTGGATCAGACGGAGCTTTTCGAGCAGTGCCAGCGTTACTACGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACg < 2:386266/140‑1 (MQ=255) gACGGAGCTTTTCGAGCAGTGCCAGCGTTACTACGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTAt > 2:156850/1‑140 (MQ=255) ggAGCTTTTCGAGCAGTGCCAGCGTTACTACGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCCCACGGCGTGGTATGgc > 2:589999/1‑140 (MQ=255) actacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAg > 1:454399/1‑140 (MQ=255) actacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAg > 2:844649/1‑140 (MQ=255) actacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAg < 2:454399/140‑1 (MQ=255) ctacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGc < 1:19967/140‑1 (MQ=255) ctacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGc < 2:730799/140‑1 (MQ=255) ctacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGc < 2:446689/140‑1 (MQ=255) tacGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCt < 1:156850/140‑1 (MQ=255) acGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTg < 2:561231/140‑1 (MQ=255) ccAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTAc < 1:320634/140‑1 (MQ=255) atatCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGc < 1:466081/132‑1 (MQ=255) atatCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGc > 2:466081/1‑132 (MQ=255) atatCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTg > 1:847980/1‑140 (MQ=255) aCGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGggc < 1:541780/140‑1 (MQ=255) aCGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGggc > 2:577818/1‑140 (MQ=255) aCGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGggc < 1:754288/140‑1 (MQ=255) gCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGggcgg > 1:576198/1‑140 (MQ=255) cGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCt < 1:577818/140‑1 (MQ=255) ttCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGa > 1:821453/1‑140 (MQ=255) ttCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCCCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGa > 1:710409/1‑140 (MQ=255) gccTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAg < 2:847980/140‑1 (MQ=255) acCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGaa < 2:311297/140‑1 (MQ=255) cGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATc > 1:567045/1‑140 (MQ=255) tGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACAcc < 2:576198/140‑1 (MQ=255) ttAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCg > 2:816306/1‑140 (MQ=255) tAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTa < 2:757307/126‑1 (MQ=255) tAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGacac > 1:797964/1‑132 (MQ=255) tAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGacac < 2:797964/132‑1 (MQ=255) tAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCGa > 1:507843/1‑140 (MQ=255) tAACTTACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAAATGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTa > 1:757307/1‑126 (MQ=255) tACCAGTCGATATGATAGCCACTTCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCGAATAac < 1:844649/140‑1 (MQ=255) ttCTCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCGAATAACACCCGCATGATGGCTGACGCTa < 1:552001/140‑1 (MQ=255) | AGTGGATCAGACGGAGCTTTTCGAGCAGTGCCAGCGTTACTACGCCAATATCCTGACGCCGTTCAGCCGCCTGGTAACACCGAAAATTACCGAGATCCTGGGCTTTAACTTACCAGTCGATATGATAGCCACTTCCCACGGCGTGGTATGGCGCGATAACCCGACGCAAATTGTCGAGCTGTACCTGAAATGGGCGGCTGATTATCAGGAAGACAGAATCACCATTTTCTACGACACCATGTCGAATAACACCCGCATGATGGCTGACGCTA > NC_000913/2833018‑2833289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |