Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,846,372	T→C	T34A (ACT→GCT)	hycE ←	formate hydrogenlyase subunit HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,846,372	0	T	C	100.0%	188.9 / NA	53	T34A (ACT→GCT)	hycE	formate hydrogenlyase subunit HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

AGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCACGCCGCCAGCAAGGAG  >  NC_000913/2846239‑2846507                                                                                                                                      |                                                                                                                                        aGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTg                                                                                                                                   >  2:770722/1‑140 (MQ=255)  gCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGa                                                                                                                                  >  2:506993/1‑140 (MQ=255)   cACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGAt                                                                                                                                 >  2:207766/1‑140 (MQ=255)    aCGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATc                                                                                                                                >  1:128538/1‑140 (MQ=255)    aCGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATc                                                                                                                                >  1:124072/1‑140 (MQ=255)     cGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCt                                                                                                                               <  1:646033/140‑1 (MQ=255)     cGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCt                                                                                                                               <  1:580759/140‑1 (MQ=255)       tagtaAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCttt                                                                                                                             <  2:732201/140‑1 (MQ=255)               ggCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGcc                                                                                                                     <  1:859874/140‑1 (MQ=255)                 cGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAg                                                                                                                   >  1:309484/1‑140 (MQ=255)                 cGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAg                                                                                                                   >  1:367559/1‑140 (MQ=255)                 cGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAg                                                                                                                   >  2:590607/1‑140 (MQ=255)                            ttCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAg                                                                                                        >  1:510962/1‑140 (MQ=255)                             tCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGc                                                                                                       >  1:138137/1‑140 (MQ=255)                                gTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCacg                                                                                                    <  2:510962/140‑1 (MQ=255)                                  ttGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCacgac                                                                                                  <  1:506993/140‑1 (MQ=255)                                   tGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCg                                                                                                                <  1:16909/125‑1 (MQ=255)                                   tGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCg                                                                                                                >  2:16909/1‑125 (MQ=255)                                     cGTTCGTCGTTACCAAACAGCACCGACAGCCAACCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGcc                                                                                               <  1:620318/140‑1 (MQ=255)                                          gtcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTATTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaa                                                                                          <  2:786331/140‑1 (MQ=255)                                          gtcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaa                                                                                          <  1:770722/140‑1 (MQ=255)                                          gtcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaa                                                                                          <  1:207766/140‑1 (MQ=255)                                           tcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaaa                                                                                         >  1:708517/1‑140 (MQ=255)                                           tcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaaa                                                                                         >  2:32561/1‑140 (MQ=255)                                           tcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaaa                                                                                         >  1:435850/1‑140 (MQ=255)                                                         cACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcg                                                                           <  2:138137/140‑1 (MQ=255)                                                         cACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcg                                                                           <  2:124072/140‑1 (MQ=255)                                                         cACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcg                                                                           <  2:128538/140‑1 (MQ=255)                                                            cGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcg                                                                           <  1:813496/137‑1 (MQ=255)                                                            cGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcg                                                                           >  2:813496/1‑137 (MQ=255)                                                                   cagccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCAGGAAATGCCTCATTCAGCGCGGCGAGATa                                                                 >  2:311586/1‑140 (MQ=255)                                                                      ccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGTGCGGCGAGATAATg                                                              <  2:309484/140‑1 (MQ=255)                                                                       caCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGt                                                             <  1:590607/140‑1 (MQ=255)                                                                            tGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGAcc                                                        >  2:857466/1‑140 (MQ=255)                                                                            tGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGGGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGCCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGAcc                                                        >  1:201766/1‑140 (MQ=255)                                                                                tGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCgag                                                                    >  2:818084/1‑124 (MQ=255)                                                                                tGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCgag                                                                    <  1:818084/124‑1 (MQ=255)                                                                                tGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAAt                                                    >  2:726318/1‑140 (MQ=255)                                                                                  tagtaAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAAttt                                                  <  1:230134/140‑1 (MQ=255)                                                                                   agtaAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAAtttt                                                 >  2:519992/1‑140 (MQ=255)                                                                                   agtaAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAAtttt                                                 >  2:76119/1‑140 (MQ=255)                                                                                        aGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTttcttc                                            >  2:84915/1‑140 (MQ=255)                                                                                           aaCTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGa                                         <  1:857466/140‑1 (MQ=255)                                                                                            aCTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGAc                                        <  1:726318/140‑1 (MQ=255)                                                                                             cTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACa                                       <  1:84915/140‑1 (MQ=255)                                                                                               ccaccaCTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATg                                     <  1:32561/140‑1 (MQ=255)                                                                                                     cTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCaaa                               <  2:435850/140‑1 (MQ=255)                                                                                                     cTTCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCaaa                               <  1:224978/140‑1 (MQ=255)                                                                                                      ttCCGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAAc                              <  1:714335/140‑1 (MQ=255)                                                                                                        ccGGCAGGTAGTTCACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAActc                            >  1:845223/1‑140 (MQ=255)                                                                                                                     cACCTTTACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCAcgc               >  1:571856/1‑140 (MQ=255)                                                                                                                     cACCTTTACGGTGACAGCCAGCTGATCTGTGGTCTGCCAGGCGTGGTCCACCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCAcgc               <  2:201766/140‑1 (MQ=255)                                                                                                                          ttACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCACGCCGCCa          >  1:642263/1‑140 (MQ=255)                                                                                                                           tACGGTGACAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCACGCCGCCAg         >  1:490830/1‑140 (MQ=255)                                                                                                                                  aCAGCCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCACGCCGCCAGCAAGgag  >  1:789595/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGATAATGTTGACCTAATTTTTCTTCAGACATGCTCAAACTCTCTTTAATCACGCCGCCAGCAAGGAG  >  NC_000913/2846239‑2846507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑