Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,343	(A)8→7	intergenic (‑165/‑44)	mltA ← / → metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,336	0	A	.	100.0%	305.5 / NA	71	intergenic (‑158/‑51)	mltA/metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/42);  total (29/42)

AAAAATAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAT  >  NC_000913/2947198‑2947471                                                                                                                                           |                                                                                                                                        aaaaaTAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTaa                                                                                                                                        >  2:43801/1‑140 (MQ=255)        aGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTaaaaaaagg                                                                                                                                 <  2:4865/140‑3 (MQ=255)               aCGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATg                                                                                                                         >  1:1232210/1‑140 (MQ=255)                 gAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGaa                                                                                                                       <  2:1232210/140‑1 (MQ=255)                      tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                                  <  1:18196/140‑1 (MQ=255)                      tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                                  <  1:353008/140‑1 (MQ=255)                      tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                                  <  2:1155178/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTTGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:534581/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  1:346848/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  1:960746/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:1007360/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:1150444/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:799692/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:1176418/140‑1 (MQ=255)                        aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                                <  2:873828/140‑1 (MQ=255)                          aaaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGc                                                                                                              <  2:794955/140‑1 (MQ=255)                           aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                             <  2:260266/140‑1 (MQ=255)                           aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                             <  1:43801/140‑1 (MQ=255)                            aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCgg                                                                                                            <  2:450509/140‑1 (MQ=255)                            aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCgg                                                                                                            >  2:1216129/1‑140 (MQ=255)                             ccgccgCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGa                                                                                                           >  1:230179/1‑140 (MQ=255)                             ccgccgCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGa                                                                                                           >  2:1239031/1‑140 (MQ=255)                              cgccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                                  <  2:1186259/132‑1 (MQ=255)                              cgccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                                  >  1:1186259/1‑132 (MQ=255)                              cgccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAg                                                                                                          >  2:396375/1‑140 (MQ=255)                                ccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGta                                                                                                        >  1:618246/1‑140 (MQ=255)                                 cgcgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGtat                                                                                                       >  1:464993/1‑140 (MQ=255)                                   cgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                             >  2:111427/1‑132 (MQ=255)                                   cgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                             <  1:111427/132‑1 (MQ=255)                                     tttAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTg                                                                                                   <  1:1239031/140‑1 (MQ=255)                                        aatgaaatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTgcgc                                                                                                <  2:319695/140‑1 (MQ=255)                                              atgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCAc                                                                                          >  1:881004/1‑140 (MQ=255)                                               tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACg                                                                                         >  2:317973/1‑140 (MQ=255)                                                   cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                                     <  1:317973/140‑1 (MQ=255)                                                      ccTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                           <  2:590030/131‑1 (MQ=255)                                                      ccTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                           >  1:590030/1‑131 (MQ=255)                                                        tctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCgggg                                                                                >  2:987821/1‑140 (MQ=255)                                                           cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                           >  1:852315/1‑126 (MQ=255)                                                           cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                           <  2:852315/126‑1 (MQ=255)                                                           cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTgg                                                                             >  1:294575/1‑140 (MQ=255)                                                            ccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGa                                                                            >  2:111077/1‑140 (MQ=255)                                                             cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAGCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                           <  1:731492/140‑1 (MQ=255)                                                             cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                           <  1:396375/140‑1 (MQ=255)                                                             cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                           <  2:294575/140‑1 (MQ=255)                                                              tGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagc                                                                          <  1:1216129/140‑1 (MQ=255)                                                               gACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagca                                                                         <  2:597063/140‑1 (MQ=255)                                                                aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagcag                                                                        >  1:117553/1‑140 (MQ=255)                                                                aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagcag                                                                        >  2:1180824/1‑140 (MQ=255)                                                                 cGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagcagc                                                                       <  1:1269956/140‑1 (MQ=255)                                                                      aTTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGt                                                                  <  2:543879/140‑1 (MQ=255)                                                                                      aGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                  <  2:82604/140‑1 (MQ=255)                                                                                      aGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                  <  2:1154444/140‑1 (MQ=255)                                                                                      aGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                  <  2:1030380/140‑1 (MQ=255)                                                                                         cTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAc                                               >  1:812407/1‑140 (MQ=255)                                                                                            tAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                            >  1:809673/1‑140 (MQ=255)                                                                                             aGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa                                           <  2:1110843/140‑1 (MQ=255)                                                                                             aGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa                                           <  2:809673/140‑1 (MQ=255)                                                                                               tGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAg                                         <  2:812407/140‑1 (MQ=255)                                                                                                  tttgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                      >  2:154558/1‑140 (MQ=255)                                                                                                    tgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                    <  2:230179/140‑1 (MQ=255)                                                                                                            ttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                            >  2:947640/1‑140 (MQ=255)                                                                                                            ttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                            <  2:675184/140‑1 (MQ=255)                                                                                                            ttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                            >  1:289913/1‑140 (MQ=255)                                                                                                                  aTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                      <  1:987821/140‑1 (MQ=255)                                                                                                                      tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCTAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                >  2:256362/1‑126 (MQ=255)                                                                                                                      tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCTAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                <  1:256362/126‑1 (MQ=255)                                                                                                                          aaaTCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa              <  2:618246/140‑1 (MQ=255)                                                                                                                             tCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc           >  1:436012/1‑140 (MQ=255)                                                                                                                             tCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc           >  2:8831/1‑140 (MQ=255)                                                                                                                                    tCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa    >  1:882376/1‑140 (MQ=255)                                                                                                                                     cGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa   >  2:556137/1‑140 (MQ=255)                                                                                                                                     cGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa   <  2:289913/140‑1 (MQ=255)                                                                                                                                      ggggT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt  >  1:196517/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        AAAAATAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAT  >  NC_000913/2947198‑2947471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑