Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,860,630	(C)5→4	coding (348/894 nt)	yidL →	putative DNA‑binding transcriptional regulator YidL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,860,626	0	C	.	100.0%	244.3 / NA	57	coding (344/894 nt)	yidL	putative DNA‑binding transcriptional regulator YidL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (37/20);  total (37/20)

AAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860488‑3860760                                                                                                                                            |                                                                                                                                       aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:121626/1‑140 (MQ=255) aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAcc                                                                                                                                       >  1:766040/1‑140 (MQ=255)    gATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAcccca                                                                                                                                    <  1:1018472/140‑2 (MQ=255)      tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  1:913320/140‑3 (MQ=255)      tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAccccacc                                                                                                                                  >  2:1050930/1‑137 (MQ=255)      tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAccccacc                                                                                                                                  >  2:506329/1‑137 (MQ=255)       gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  1:883853/140‑5 (MQ=255)       gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  2:236598/140‑5 (MQ=255)        tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  1:376027/1‑140 (MQ=255)        tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  1:227243/1‑140 (MQ=255)        tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  2:855378/1‑140 (MQ=255)            aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  2:460222/140‑1 (MQ=255)            aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  1:697666/140‑1 (MQ=255)              aaTTCTTTTAAAGAATTGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         >  2:112303/1‑140 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         <  2:227243/140‑1 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         >  1:462454/1‑140 (MQ=255)               aTTCTTTTAAAGAATTGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTatgatg                                                                                                                        <  1:112303/140‑1 (MQ=255)                    tttAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGatat                                                                                                                   <  1:1118919/140‑1 (MQ=255)                         aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  2:462454/140‑1 (MQ=255)                                              tGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttattt                                                                                         >  2:78132/1‑140 (MQ=255)                                                cATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttatttat                                                                                       >  2:1197385/1‑140 (MQ=255)                                                 aTGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTtata                                                                                      >  2:131063/1‑140 (MQ=255)                                                 aTGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTtata                                                                                      >  2:1265176/1‑140 (MQ=255)                                                      aaCTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGc                                                                                 <  1:855378/140‑1 (MQ=255)                                                        cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGa                                                                               <  2:819701/140‑1 (MQ=255)                                                          gTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCg                                                                                               <  2:1270342/122‑1 (MQ=255)                                                          gTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCg                                                                                               >  1:1270342/1‑122 (MQ=255)                                                              tataTTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  1:296721/140‑1 (MQ=255)                                                              tataTTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  2:175273/140‑1 (MQ=255)                                                                   tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    <  1:699135/140‑1 (MQ=255)                                                                   tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  2:482997/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCGCCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         >  2:1038919/1‑133 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  1:341480/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  1:62643/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  2:722593/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGA‑AACAGTACTGGATGGAATTTA‑CCCCGCCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  1:1038919/133‑1 (MQ=255)                                                                        aaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGaa                                                               <  1:1265176/140‑1 (MQ=255)                                                                          gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACt                                                             >  2:1085121/1‑140 (MQ=255)                                                                          gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACt                                                             >  1:180749/1‑140 (MQ=255)                                                                               tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  2:280530/1‑140 (MQ=255)                                                                                       cACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  1:1197385/140‑1 (MQ=255)                                                                                        aCTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTg                                               >  2:172033/1‑140 (MQ=255)                                                                                          tctTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAg                                             >  2:787688/1‑140 (MQ=255)                                                                                           ctTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGt                                            >  1:1041381/1‑140 (MQ=255)                                                                                            ttATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGtt                                           >  2:172120/1‑140 (MQ=255)                                                                                              aTCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  2:1178051/133‑1 (MQ=255)                                                                                              aTCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                >  1:1178051/1‑133 (MQ=255)                                                                                                 aCTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  2:676466/130‑1 (MQ=255)                                                                                                 aCTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                >  1:676466/1‑130 (MQ=255)                                                                                                  cTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCGGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAAc                                     >  2:1098832/1‑140 (MQ=255)                                                                                                   tgtgAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACt                                    >  2:1202810/1‑140 (MQ=255)                                                                                                    gtgAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  1:254968/1‑140 (MQ=255)                                                                                                      gAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCa                                 >  1:422505/1‑140 (MQ=255)                                                                                                      gAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCa                                 >  1:745952/1‑140 (MQ=255)                                                                                                      gAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCa                                 >  2:1053475/1‑140 (MQ=255)                                                                                                        aGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCAcc                               >  1:439317/1‑140 (MQ=255)                                                                                                        aGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCAcc                               <  1:78132/140‑1 (MQ=255)                                                                                                             tAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  2:422505/140‑1 (MQ=255)                                                                                                             tAGTCTGGG‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          >  1:1075521/1‑140 (MQ=255)                                                                                                                    gg‑AACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATaaa                   >  1:155829/1‑140 (MQ=255)                                                                                                                               tggatggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTg         >  1:217254/1‑140 (MQ=255)                                                                                                                                 gatggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGc       >  1:977042/1‑140 (MQ=255)                                                                                                                                    ggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGt    <  1:121626/140‑1 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:176698/1‑140 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:667451/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       AAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGG‑AACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860488‑3860760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑