Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,728,1101.C95.5% 239.6 / 2.9 67coding (1262/1455 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (25/39);  total (25/42)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC  >  NC_000913/3727973‑3728240
                                                                                                                                            |                                                                                                                                   
gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑cc                                                                                                                                  <  2:825765/140‑1 (MQ=255)
   gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑ccccc                                                                                                                               <  2:188231/140‑1 (MQ=255)
     tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑ccccccc                                                                                                                             <  1:450442/140‑1 (MQ=255)
      aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑cccccccc                                                                                                                            <  1:760536/140‑1 (MQ=255)
        gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑cccccccccg                                                                                                                          >  1:113322/1‑138 (MQ=255)
          ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑cccccccccgta                                                                                                                        >  1:1075727/1‑136 (MQ=255)
             aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACgg                                                                                                                      <  1:765135/140‑1 (MQ=255)
                  gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATc                                                                                                                 <  2:250714/140‑1 (MQ=255)
                  gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATc                                                                                                                 <  2:1068096/140‑1 (MQ=255)
                      cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAgc                                                                                                             >  2:189582/1‑140 (MQ=255)
                          tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                         <  1:1213253/140‑1 (MQ=255)
                          tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                         <  1:223313/140‑1 (MQ=255)
                          tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                         <  1:169804/140‑1 (MQ=255)
                             ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                                                      <  1:1208214/140‑1 (MQ=255)
                                  aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                 >  1:759378/1‑140 (MQ=255)
                                  aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGAAATCCAGCTGCTGACCGCTg                                                                                                 >  1:1259661/1‑140 (MQ=255)
                                   tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                >  2:161908/1‑140 (MQ=255)
                                   tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                >  1:307766/1‑140 (MQ=255)
                                   tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCCTACGGTAATCGAGCTGCTGACCGCTgg                                                                                                >  2:944438/1‑139 (MQ=255)
                                     cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                              >  1:1139456/1‑140 (MQ=255)
                                     cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                              <  2:692157/140‑1 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                            >  1:137380/1‑140 (MQ=255)
                                        cTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                           >  1:921420/1‑140 (MQ=255)
                                        cTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                           >  2:843750/1‑140 (MQ=255)
                                           ttCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGTCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                        <  1:289957/140‑1 (MQ=255)
                                           ttCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                        >  1:466433/1‑140 (MQ=255)
                                             cTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                      <  1:494751/140‑1 (MQ=255)
                                                aCGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                   >  1:189253/1‑140 (MQ=255)
                                                 cGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                  <  2:757591/140‑1 (MQ=255)
                                                  ggTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                 <  1:320316/140‑1 (MQ=255)
                                                  ggTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                 <  1:164009/140‑1 (MQ=255)
                                                  ggTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                 <  2:773754/140‑1 (MQ=255)
                                                  ggTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAACGGAGCTGCTGCCCGCTGATATCCGCCAGCATCt                                                                                 <  2:113322/140‑1 (MQ=255)
                                                    tAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCTCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                               <  2:1173851/140‑1 (MQ=255)
                                                    tAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                               <  1:185400/140‑1 (MQ=255)
                                                     aGTTGCGGCAACAATTCAATTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                          <  2:1094998/129‑1 (MQ=255)
                                                     aGTTGCGGCAACAATTCAATTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                          >  1:1094998/1‑129 (MQ=255)
                                                      gTTGCGGCAACAATTCAA‑TGCGCTACTTCTCTGGATTCGCCGCGTTCTGCGCCAGCCTTGCTACGCCCAGTGCTGCCCCCCCACC‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                             <  2:1132022/140‑1 (MQ=255)
                                                      gTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                             <  1:1183836/140‑1 (MQ=255)
                                                      gTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                             <  1:1206076/140‑1 (MQ=255)
                                                       ttGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                            <  2:921420/140‑1 (MQ=255)
                                                         gCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                          <  1:674099/140‑1 (MQ=255)
                                                          cGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                 <  2:613264/132‑1 (MQ=255)
                                                          cGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                 >  1:613264/1‑132 (MQ=255)
                                                              aacaaTTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                     <  1:876799/140‑1 (MQ=255)
                                                                   ttCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                <  2:591102/140‑1 (MQ=255)
                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                         <  2:626109/126‑1 (MQ=255)
                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                         >  1:626109/1‑126 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                          <  2:1139456/140‑1 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                          <  2:1075727/140‑1 (MQ=255)
                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                         <  1:386227/140‑1 (MQ=255)
                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                         >  1:1112039/1‑140 (MQ=255)
                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                         <  1:516892/140‑1 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCCCTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                         >  2:36281/1‑140 (MQ=255)
                                                                             cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                       <  1:843750/140‑1 (MQ=255)
                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGC‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc                                                      <  2:307766/140‑1 (MQ=255)
                                                                               aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                     >  2:1196323/1‑140 (MQ=255)
                                                                               aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                     <  2:137380/140‑1 (MQ=255)
                                                                                     ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                               >  2:577756/1‑140 (MQ=255)
                                                                                     ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                               <  1:45343/140‑1 (MQ=255)
                                                                                     cgggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACC‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                               <  1:944438/138‑1 (MQ=255)
                                                                                       ggATCCACCGCCTTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCACCA‑TC‑CCCCCCCCGGACGGTAAGCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCAATCa                                             <  2:1187367/140‑1 (MQ=255)
                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                          >  2:323467/1‑140 (MQ=255)
                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                          >  2:1090136/1‑140 (MQ=255)
                                                                                            ccccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCGGGCCCCACACC‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                        <  2:1259661/138‑1 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACCCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGaa                                       >  2:803498/1‑140 (MQ=255)
                                                                                                    tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                <  1:9995/140‑1 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                            >  2:1033997/1‑140 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGCACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                            >  1:178420/1‑140 (MQ=255)
                                                                                                         gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                           <  1:577756/140‑1 (MQ=255)
                                                                                                            aGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGTCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                        <  2:1112039/140‑1 (MQ=255)
                                                                                                                    tgcGCCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                <  1:1196323/140‑1 (MQ=255)
                                                                                                                       gcCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa             <  2:178420/140‑1 (MQ=255)
                                                                                                                       gcCCAGTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa             <  1:594574/140‑1 (MQ=255)
                                                                                                                            gTGCTGGCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc        <  2:890819/140‑1 (MQ=255)
                                                                                                                                  gCCCCACA‑TC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc  >  2:1038829/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                   
GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAA‑TGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC  >  NC_000913/3727973‑3728240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑