Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,375,828	IS186 (–) +4 bp	coding (132‑135/963 nt)	menC ←	o‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2375828 =	0 (0.000)	48 (0.660)	35/268	0.9	100%	coding (135/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region
*	?	NC_000913	= 2375831	0 (0.000)	67 (0.930)	51/268	0.2	100%	coding (132/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	= 2515617	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region

GCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2375955‑2375828 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  >  NC_000913/2514273‑2514415                                                                                                                                                                                                                                                                                  GCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCCCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGGAGGGGGGGGGGGGATCTCCCATAAGCGC                                                                                                                                     >  2:1036314/1‑140  agacagGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCT                                                                                                                                    <  1:239015/134‑1        GGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTA                                                                                                                              <  1:846613/140‑1         GTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAA                                                                                                                             <  1:359030/140‑1            TACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGTGCTAACTTAAGGG                                                                                                                          <  1:912042/140‑1               CGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGGGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTG                                                                                                                       >  1:243432/1‑140                 CTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCT                                                                                                                                    <  1:688571/125‑1                 CTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGGGGGGGGGGATCTCCCATAAGCGCT                                                                                                                                    >  2:688571/1‑125                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGGTTAAAAACCC‑CGACGGGCTGGATGTTTGCCTGGCGGAAGGCGAGCGCGGAAGGTGGGGGGGGGTCTCCCATAAGCGCGAACTTAAGGGTGGTGGT                                                                                                                   >  2:742296/1‑140                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGGGGGCGCGAAAGGGGGGGGGAGGTCTCCCATAAGCGCTAACTTAAGGGGTGTGGg                                                                                                                   >  2:359030/1‑139                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGGAGGGGGGGGGGGGATCTCCCCTAAGCGCTAACTTAAGGGGTGTGGg                                                                                                                   >  2:846613/1‑139                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGT                                                                                                                         <  2:660992/134‑1                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGGGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGT                                                                                                                         >  1:660992/1‑134                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAAACCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGGGCGCGGAGGGGGGGGGGGGATCTCCCCTAAGCGCTAACTTAAGGGgctgtct                                                                                                                   >  2:239015/1‑133                   GGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAAACCCCGACGGGGTGTATGTTTTCCTGCGTGAAGGCGAGCGCGAAGGGGGGGGGGGGATCTCCCATAAGTGCTAACTTTAGGGGTGTGGg                                                                                                                   >  2:912042/1‑139                         TCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACG                                                                                                             <  2:535531/140‑1                         TCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACG                                                                                                             <  2:588076/140‑1                          CCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                                                                                            <  1:742296/140‑1                                   CGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCCGCGTGAAGGGGAGGGCGGAGGGGGGGGGGGGATCTCCCCTAAGCGCGAACTTTAGGGTTGGGGGATTACGCCCGAGATGt                                                                                                   >  2:59244/1‑139                                           TGGTTCTGCGCGACAGGC‑GGTTAAAAAACCGCGACGGGCGGTTTGTTTGCCTGCGTGAAGGCGAGCGCGGAGGGGGGGGGGAGATCTCCCATAAGCGCTGACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGT                                                                                           >  2:1029415/1‑140                                               TCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCG                                                                                       <  1:939329/140‑1                                                  GCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATG                                                                                    >  1:46317/1‑140                                                       ACAGGC‑GGTTAAAAAACCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGGGCGCGAAGGGTGGGGGGGGATCTCCCATAAGCGCTAACTTAAGGGGTGTGGGATTACGCCTGATATGATTTAACGTGCCGATGAATTA                                                                               >  1:491895/1‑140                                                         AGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                             <  1:802044/140‑1                                                         AGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                             <  1:413340/140‑1                                                            C‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTC                                                                          <  1:450992/140‑1                                                                                GGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTG                                                                >  2:496754/1‑131                                                                                GGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTG                                                                <  1:496754/131‑1                                                                                      ATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 <  1:652611/140‑1                                                                                      ATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 >  2:652611/1‑140                                                                                      ATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 <  2:1148163/140‑1                                                                                      ATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 <  2:46317/140‑1                                                                                      ATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 <  2:243432/140‑1                                                                                       TGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCC                                                <  1:713612/140‑1                                                                                           TGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA                                            >  2:14279/1‑140                                                                                               TGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 >  1:674941/1‑131                                                                                               TGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                 <  2:674941/131‑1                                                                                                 CGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTA                                      >  1:22185/1‑140                                                                                                  GTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGG                                        <  2:212706/137‑1                                                                                                  GTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGG                                        >  1:212706/1‑137                                                                                                         CGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAA                              <  1:1248874/140‑1                                                                                                                   GGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATA                    <  2:885236/140‑1                                                                                                                      TGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTT                 >  2:575897/1‑140                                                                                                                      TGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTT                 <  1:61587/140‑1                                                                                                                              GATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGT         >  2:521449/1‑140                                                                                                                               agCTCCCATAAGTGCTAACTTAAGGGTTGCGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTA        >  2:624267/3‑140                                                                                                                               ATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTA        >  1:534876/1‑140                                                                                                                                TCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGGCCGAAGAACTGGATACTTCGGCACGTAA       >  2:1171418/1‑140                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  2:547095/140‑1                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  1:537872/140‑1                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  1:553213/140‑1                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  1:1213883/140‑1                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  >  1:777747/1‑140                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  1:335178/140‑1                                                                                                                                     CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  <  2:163472/140‑1                                                                                                                                                                                                                                                                                  GCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGC‑GGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2375955‑2375828 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCG  >  NC_000913/2514273‑2514415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.