Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA32.6% 88.6 / 25.0 49intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (29/4);  new base A (0/16);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.45e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACC  >  NC_000913/752986‑753262
                                                                                                                                           |                                                                                                                                         
gggTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCatat                                                                                                                                           <  2:431901/140‑1 (MQ=255)
     ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                                      >  1:964144/1‑140 (MQ=255)
          tGCGACTCCCATGAATTTTTTAATTGAAAGTATTGGGTTGTTGATAATTTGAGTTTTTTTTTTTTTTTTAAATATTTATATGGGTCTTTTAATGGTTTTTATTTTTAAATTTTTTTGTGTTTAGGAAAAAAAAAACCAAc                                                                                                                                 <  2:964144/140‑1 (MQ=255)
           gCGACTACCATGAATTTTTTAATTGAAAGTATTGGGTTGCTAATAATTTGGGTTTTTTTTTTTTTTTTAAATATCTATATAGTTCTTTTAATGGATTTTATTTTTACAATTTTTTGTTTTTAGGCAAATAAAAATCAAcc                                                                                                                                <  2:89963/140‑1 (MQ=255)
           gCGACTACCATGAATTTTTTAATTAAAAGTATTGGGTTGCTGATATTTTGAGCTTTTTTTTTTTTTTTAAATATCTATATAGTTCTGTTAATGTTTTTTATTTTTACAATTTTTTGTTTTTAGGAATAAAAAAATCAAcc                                                                                                                                <  2:1194585/140‑1 (MQ=255)
             gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                              >  1:1050895/1‑140 (MQ=255)
             gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                              >  2:151181/1‑140 (MQ=255)
             gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                              >  1:549223/1‑140 (MQ=255)
               cTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGcc                                                                                                                            >  1:89963/1‑140 (MQ=255)
                            tttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcggcgg                                                                                                               >  2:1170691/1‑140 (MQ=255)
                               aaTTGAAATTTTTGGTTTGTTAATAATTTGAGTTTTTTTTTTTTTTTAAAATATTTATATAGTTTTGTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGtt                                                                                                            <  2:1139540/140‑1 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTaaaaa                                                                                                       >  2:870116/1‑139 (MQ=255)
                                                tGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTa                                                                                           >  1:1028316/1‑140 (MQ=255)
                                                tGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACATa                                                                                           >  1:1238427/1‑140 (MQ=255)
                                                 gttaaTAATTTGACTTTTTCTATTTTTTTTAAATATCTATATAGGTCTTTTAAGGGATTTTATTTTTAAAATTTTTTGTTTTTAGGCATAAAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                          <  2:485427/136‑1 (MQ=255)
                                                 gCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAg                                                                                          >  2:1055143/1‑140 (MQ=255)
                                                 gCTAATATTTTGAGTTTTTTTTTTTTTTTTAAATTTTTATATAGGTTTGTTAATGGTTTTTTTTTTTAAATTTTTTTTTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                          <  2:531808/140‑1 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                         >  1:1089196/1‑140 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                         >  2:968040/1‑140 (MQ=255)
                                                    aatatTTTGAGTTTTTTTTTTTTTTTTAAATATCTATATAGTTTTGTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGAAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCaa                                                                                       <  2:621698/139‑1 (MQ=255)
                                                            gAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                               <  1:1170691/140‑1 (MQ=255)
                                                                         ttttttAAATATCTATATGGGTTTTTTAATGTTTTTTTTTTTTAAAATTTTTTGTTTTTAGCAATAAAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                  <  2:1028316/139‑1 (MQ=255)
                                                                         tttttaAAATTTTTATATGGGTCTTTAAATGGTTTTTTTTTTTACATTTTTTTTTTTTTAGGCATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                  <  2:613388/139‑1 (MQ=255)
                                                                         tttttaAAATATGTATATAGTTCTTTTAGTGGATTTTATTTTTACTTTTTTTTGTGTTAAGGAAAAAAAAAAGCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                  <  2:1238427/139‑1 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                               >  1:1079559/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                               >  1:818617/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                               >  1:16139/1‑140 (MQ=255)
                                                                                          taGGTCTGTAAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGCAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                 <  2:950664/140‑1 (MQ=255)
                                                                                             tttttttAATGTTTTTTTTTTTTATATTTTTTTGTTTTTAGAAAAAAAAAAACCACCCCGCC‑‑ATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                                            <  2:1246286/135‑1 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                              >  1:956567/1‑140 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                              >  1:910400/1‑140 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                              >  1:213394/1‑140 (MQ=255)
                                                                                              tCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttttt                                             >  1:596592/1‑140 (MQ=255)
                                                                                              tCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttttt                                             >  1:950664/1‑140 (MQ=255)
                                                                                               tttttAATGGTTTTTTTTTTTACATTTTTTTTTTTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                                            <  2:725166/137‑1 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                           >  1:409053/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                           >  2:107563/1‑140 (MQ=255)
                                                                                                 tttAATGTTTTTTTTTTTTACATTTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                          <  2:16139/139‑1 (MQ=255)
                                                                                                 tttAATGTTTTTTTTTTTTAAATTTTTTTTTTTTTAGAAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                          <  2:956567/139‑1 (MQ=255)
                                                                                                 tttAATGTTTTTTTTTTTTAAATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                          <  1:870116/139‑1 (MQ=255)
                                                                                                 tttAATGTATTTTATTTTTACAATTTTTTGTTTTGAGAAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                          <  2:213394/139‑1 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                         >  2:647984/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                         >  1:725166/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                         >  1:71672/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                         >  1:662929/1‑140 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCC‑‑ATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                       >  1:1246286/1‑140 (MQ=255)
                                                                                                    aaTGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                       <  2:596592/140‑1 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                       <  1:151181/140‑1 (MQ=255)
                                                                                                           tttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGtata                                >  1:456432/1‑140 (MQ=255)
                                                                                                            ttttttttAAATTTTTTTTTTTTTAGGAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAc                               <  2:818617/137‑1 (MQ=255)
                                                                                                             tttttttAAATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACa                              <  2:1079559/138‑1 (MQ=255)
                                                                                                              ttttttACATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAg                             <  2:1200577/139‑1 (MQ=255)
                                                                                                                   tACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTTATATCCTGTATACAGCTGAc                        >  2:848517/1‑140 (MQ=255)
                                                                                                                         tttttGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGAc                  <  1:578519/140‑1 (MQ=255)
                                                                                                                             tgtgTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc              <  1:7328/140‑1 (MQ=255)
                                                                                                                              gtttttAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCa             <  2:986035/137‑1 (MQ=255)
                                                                                                                               tgtTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCaa            <  1:736750/140‑1 (MQ=255)
                                                                                                                                   tAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCtt        <  1:968040/140‑1 (MQ=255)
                                                                                                                                   tAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCtt        <  2:409053/140‑1 (MQ=255)
                                                                                                                                        ataaAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAc   <  2:1050895/140‑1 (MQ=255)
                                                                                                                                        ataaAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAc   <  2:1008934/140‑1 (MQ=255)
                                                                                                                                         tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAcc  >  1:1008934/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACC  >  NC_000913/752986‑753262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑