Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,401,4590TC28.6% 99.6 / 11.8 56D625G (GAT→GGT) csrDregulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (33/7);  new base C (0/16);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 5.89e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTC  >  NC_000913/3401322‑3401592
                                                                                                                                         |                                                                                                                                     
cGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGACAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATcc                                                                                                                                     >  2:847965/1‑140 (MQ=255)
      gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGc                                                                                                                               <  2:755529/140‑1 (MQ=255)
        cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                             <  1:659047/140‑1 (MQ=255)
        aaaGCCCCCTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAACCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                             <  2:253580/139‑1 (MQ=255)
              cacTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGt                                                                                                                       >  2:1240582/1‑140 (MQ=255)
              cacTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGt                                                                                                                       >  2:398204/1‑140 (MQ=255)
                 tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAac                                                                                                                    >  2:76974/1‑140 (MQ=255)
                 tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCGTGCCCGCCTGTGac                                                                                                                    >  2:777193/1‑140 (MQ=255)
                  cccATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAaca                                                                                                                   <  1:1051880/140‑1 (MQ=255)
                   ccATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAacac                                                                                                                  >  1:1110624/1‑140 (MQ=255)
                       gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcgcg                                                                                                              <  1:76974/140‑1 (MQ=255)
                       gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                              <  1:1048388/140‑1 (MQ=255)
                        agaCGCGGCGCGCTTTTTTCTACGTAAAAACGGATTAAACGGCAGGTTAACCCGAGTTTTTTTGTAAATTTTTTTTCACGTTAGTATCAAGTGGCTGGGAGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCgcgc                                                                                                             <  2:950343/140‑1 (MQ=255)
                         gaCGCAGCGCGCATTATTTTAGGTGAAAACGGATTAAACGGCAGGTTAACCCGAGTATTTTTGTAAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCt                                                                                                            <  2:706148/140‑1 (MQ=255)
                         gaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCt                                                                                                            <  1:777193/140‑1 (MQ=255)
                         gaCGCAGCGCGCATTATTCTACGGGAAAACGGATAAAACGGCGGGTTAAACCGAGTATCTTTGTAAATATTTTTCCACGTTAGTATAAAGGGGCTGTGAGGCCGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCt                                                                                                            <  2:242607/140‑1 (MQ=255)
                           cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                          >  1:242607/1‑140 (MQ=255)
                           cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                          >  1:1136230/1‑140 (MQ=255)
                           cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                          >  1:706148/1‑140 (MQ=255)
                             cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                        >  1:980286/1‑140 (MQ=255)
                               gcgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCa                                                                                                      >  1:1133861/1‑140 (MQ=255)
                                cgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAg                                                                                                     >  2:494753/1‑140 (MQ=255)
                                         ttttACGTAAAAACGGATTAAACGGCAGGTTAAACCGGGTTTTTTTGTGAATTTTTTTTCCCGTTAGTATCAAGGGGCTGGGGGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCa                                                                                            <  2:129186/137‑1 (MQ=255)
                                         ttCTACGTGAAAACGGATTAAACGGCAGGTAAAACGGTGTATCTTTGTGAATATTTTTTCACTTTAGTATCAAGTGGCTGTGAGGACGCGAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCa                                                                                            <  2:1242322/140‑1 (MQ=255)
                                           ttACGTAAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTAAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACt                                                                                          <  2:1110624/139‑1 (MQ=255)
                                              cGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGc                                                                                       >  2:775003/1‑140 (MQ=255)
                                                tGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCtt                                                                                     <  2:980286/140‑1 (MQ=255)
                                                 gAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTc                                                                                    >  2:15369/1‑140 (MQ=255)
                                                   aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                                  >  2:1203533/1‑140 (MQ=255)
                                                                ggCGGGTTAAACCGAGCATCTTTGTAAATATTTTTTCACGTTAGTAACAAGTGGCTGTGAGGCCGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                     <  2:1136230/140‑1 (MQ=255)
                                                                     gTTAACCCGAGTATTTTTGTGAATATTTTTTCACTTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                                <  1:262131/140‑1 (MQ=255)
                                                                     gTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                                >  1:430671/1‑140 (MQ=255)
                                                                               gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                      >  2:954043/1‑140 (MQ=255)
                                                                                      tgtgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGt                                               >  2:255787/1‑140 (MQ=255)
                                                                                        tgAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                             <  2:430671/140‑1 (MQ=255)
                                                                                            tatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGa                                         >  1:413314/1‑140 (MQ=255)
                                                                                             tttttttCCCGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAg                                        <  2:1133861/139‑1 (MQ=255)
                                                                                              ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                       >  2:814220/1‑140 (MQ=255)
                                                                                                       gTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCt                                  >  1:1100387/1‑136 (MQ=255)
                                                                                                       gTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTcca                              >  2:285227/1‑140 (MQ=255)
                                                                                                       gTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCt                                  <  2:1100387/136‑1 (MQ=255)
                                                                                                                aaGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCttt                     <  1:255787/140‑1 (MQ=255)
                                                                                                                 aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                    >  1:496572/1‑140 (MQ=255)
                                                                                                                  gTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGa                   <  2:701960/140‑1 (MQ=255)
                                                                                                                   gggCTGTGAGGACGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                  <  2:241293/139‑1 (MQ=255)
                                                                                                                     gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                >  1:1083614/1‑140 (MQ=255)
                                                                                                                     gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                >  1:241293/1‑140 (MQ=255)
                                                                                                                     gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                >  1:333494/1‑140 (MQ=255)
                                                                                                                     gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                >  1:387076/1‑140 (MQ=255)
                                                                                                                      cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca               >  2:316851/1‑140 (MQ=255)
                                                                                                                      cTGGGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca               <  2:434305/140‑1 (MQ=255)
                                                                                                                        gtgAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc             >  2:517003/1‑140 (MQ=255)
                                                                                                                        gtgAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc             >  1:1210351/1‑140 (MQ=255)
                                                                                                                                  gAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtct   >  1:1259911/1‑140 (MQ=255)
                                                                                                                                   aaaaaaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGa                   >  2:454614/1‑123 (MQ=255)
                                                                                                                                   aaaaaaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGa                   <  1:454614/123‑1 (MQ=255)
                                                                                                                                   aaaaaaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtctc  >  2:169824/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTC  >  NC_000913/3401322‑3401592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑