Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	561,193	A→G	E499E (GAA→GAG)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	561,193	0	A	G	100.0%	290.1 / NA	77	E499E (GAA→GAG)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/37);  total (40/37)

GATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAACCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGA  >  NC_000913/561058‑561327                                                                                                                                        |                                                                                                                                       gATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGa                                                                                                                                    <  2:715057/140‑1 (MQ=255)  aTGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGAt                                                                                                                                   >  1:201852/1‑140 (MQ=255)     aaCACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAAt                                                                                                                                <  2:487254/140‑1 (MQ=255)       cacCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCg                                                                                                                              <  2:196227/140‑1 (MQ=255)          cTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGa                                                                                                                           >  2:999081/1‑140 (MQ=255)                cACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTAcc                                                                                                                     <  1:880082/140‑1 (MQ=255)                   cAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACcaca                                                                                                                  <  2:1084879/140‑1 (MQ=255)                    aaTTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACaa                                                                                                                 >  1:34036/1‑140 (MQ=255)                             ttAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCtt                                                                                                        <  1:281295/140‑1 (MQ=255)                             ttAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGCACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCtt                                                                                                        >  1:459393/1‑140 (MQ=255)                                     ttACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTa                                                                                                <  2:23330/140‑1 (MQ=255)                                      tACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTaa                                                                                               <  2:201852/140‑1 (MQ=255)                                       aCCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTaaa                                                                                              <  1:575477/140‑1 (MQ=255)                                         cGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGa                                                                                            >  1:624434/1‑140 (MQ=255)                                          gCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGAc                                                                                           >  1:646216/1‑140 (MQ=255)                                             aTTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGt                                                                                        >  1:98337/1‑140 (MQ=255)                                              ttCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGtt                                                                                       >  1:986672/1‑140 (MQ=255)                                              ttCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGtt                                                                                       >  1:421888/1‑140 (MQ=255)                                                cGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGc                                                                                                    <  2:1025485/125‑1 (MQ=255)                                                cGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGc                                                                                                    >  1:1025485/1‑125 (MQ=255)                                                   cacaAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAg                                                                                  >  1:568516/1‑140 (MQ=255)                                                               ataCCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTca                                                                      <  2:145045/140‑1 (MQ=255)                                                                   ccTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAAt                                                                  <  2:656068/140‑1 (MQ=255)                                                                   ccTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAAt                                                                  >  2:1064435/1‑140 (MQ=255)                                                                   ccTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAAt                                                                  <  1:1064435/140‑1 (MQ=255)                                                                    cTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATc                                                                 >  2:680559/1‑140 (MQ=255)                                                                    cTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATc                                                                 >  2:992657/1‑140 (MQ=255)                                                                    cTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATc                                                                 >  2:393249/1‑140 (MQ=255)                                                                      tgatgaTGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCAc                                                               >  2:362772/1‑140 (MQ=255)                                                                        atgatgTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACtt                                                             >  2:349166/1‑140 (MQ=255)                                                                          gatgTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCGGTTAAATGTTTCACAATCACTTaa                                                           <  1:1236210/140‑1 (MQ=255)                                                                            tgTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATg                                                         >  2:1219071/1‑140 (MQ=255)                                                                             gTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGa                                                        >  2:480391/1‑140 (MQ=255)                                                                             gTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGa                                                        >  1:42334/1‑140 (MQ=255)                                                                               tGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACt                                                      >  1:291001/1‑140 (MQ=255)                                                                                   tATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTgg                                                  >  2:1122172/1‑140 (MQ=255)                                                                                    aTCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGc                                                 <  2:624434/140‑1 (MQ=255)                                                                                    aTCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGc                                                 <  1:480391/140‑1 (MQ=255)                                                                                      cgtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTc                                               >  1:385176/1‑140 (MQ=255)                                                                                       gtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCg                                              <  1:393249/140‑1 (MQ=255)                                                                                       gtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCg                                              >  1:1055838/1‑140 (MQ=255)                                                                                       gtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCg                                              <  2:34036/140‑1 (MQ=255)                                                                                       gtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCg                                              <  1:1219071/140‑1 (MQ=255)                                                                                       gtcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCg                                              <  1:387824/140‑1 (MQ=255)                                                                                        tcgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGc                                             >  2:862890/1‑140 (MQ=255)                                                                                         cgAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCt                                            <  2:421888/140‑1 (MQ=255)                                                                                          gAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCtt                                           <  1:489405/140‑1 (MQ=255)                                                                                           aaTGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTaa                                                           <  2:592774/123‑1 (MQ=255)                                                                                           aaTGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTaa                                                           >  1:592774/1‑123 (MQ=255)                                                                                           aaTGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCttt                                          >  1:709863/1‑140 (MQ=255)                                                                                             tGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCTATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTgg                                                  <  1:233202/130‑1 (MQ=255)                                                                                             tGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCTATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTgg                                                  >  2:233202/1‑130 (MQ=255)                                                                                             tGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTtat                                        <  1:362772/140‑1 (MQ=255)                                                                                             tGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTtat                                        <  1:605392/140‑1 (MQ=255)                                                                                             tGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTtat                                        <  2:42334/140‑1 (MQ=255)                                                                                              ggAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTtata                                       <  2:922049/140‑1 (MQ=255)                                                                                                  gggTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTc                                   >  1:602024/1‑140 (MQ=255)                                                                                                  gggTACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTc                                   >  2:13389/1‑140 (MQ=255)                                                                                                     tACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTgg                                >  1:85337/1‑140 (MQ=255)                                                                                                     tACGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAACCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTgg                                >  2:772977/1‑140 (MQ=255)                                                                                                       cGAATATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTa                              <  1:1247059/140‑1 (MQ=255)                                                                                                         aaTATGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTAcc                            >  2:824030/1‑140 (MQ=255)                                                                                                            atGATTACGACGGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCAt                         >  1:664712/1‑140 (MQ=255)                                                                                                                   cgacgGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAAt                  <  1:772977/140‑1 (MQ=255)                                                                                                                    gacgGTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATa                 <  1:862890/140‑1 (MQ=255)                                                                                                                        gTGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTgg             >  1:64414/1‑140 (MQ=255)                                                                                                                         tGAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGa            >  1:727946/1‑140 (MQ=255)                                                                                                                          gAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCaaaaa                   >  1:687012/1‑132 (MQ=255)                                                                                                                          gAGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCaaaaa                   <  2:687012/132‑1 (MQ=255)                                                                                                                           aGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAAt          <  1:647319/140‑1 (MQ=255)                                                                                                                           aGCATCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAAt          >  2:259335/1‑140 (MQ=255)                                                                                                                              aTCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACt       <  2:664712/140‑1 (MQ=255)                                                                                                                               tCGCGATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACtt      <  1:999081/140‑1 (MQ=255)                                                                                                                                cgcgATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCaa                      <  2:249940/123‑1 (MQ=255)                                                                                                                                cgcgATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCaa                      >  1:249940/1‑123 (MQ=255)                                                                                                                                cgcgATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTc     >  2:1081738/1‑140 (MQ=255)                                                                                                                                   gATGAGCCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGa  <  1:349166/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTTGCGTATCGTCGAATGGAGGGGTACGAATATGATTACGACGGTGAGCATCGCGATGAACCGATAATCGTGAATTACCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATGTTTCACAATCACTTAATGACTTTGGCTCGCTTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGA  >  NC_000913/561058‑561327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑