Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 568,610 T→C L99S (TTG→TCG)  emrE → multidrug/betaine/choline efflux transporter EmrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913568,6100TC100.0% 349.7 / NA 91L99S (TTG→TCG) emrEmultidrug/betaine/choline efflux transporter EmrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/57);  total (34/57)

TATTCCTACAGGGATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTT  >  NC_000913/568473‑568746
                                                                                                                                         |                                                                                                                                        
tatTCCTACAGGGATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGa                                                                                                                                        >  1:755614/1‑140 (MQ=255)
   tCCTACAGGGATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGatta                                                                                                                                     <  2:634046/140‑1 (MQ=255)
   tCCTACAGGGATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGatta                                                                                                                                     <  2:101100/140‑1 (MQ=255)
           ggATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTa                                                                                                                             <  2:476391/140‑1 (MQ=255)
              ttGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTg                                                                                                                          >  2:127748/1‑140 (MQ=255)
               tGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGt                                                                                                                         >  2:527489/1‑140 (MQ=255)
                gCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTAt                                                                                                                            >  1:1146116/1‑136 (MQ=255)
                gCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTAt                                                                                                                            <  2:1146116/136‑1 (MQ=255)
                 cTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:563749/140‑1 (MQ=255)
                 cTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:711421/140‑1 (MQ=255)
                 cTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:755614/140‑1 (MQ=255)
                 cTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:184526/140‑1 (MQ=255)
                 cTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGAGTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:771223/140‑1 (MQ=255)
                   tATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACg                                                                                                                     <  2:445787/140‑1 (MQ=255)
                     tGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGaa                                                                                                                   <  1:497561/140‑1 (MQ=255)
                      gCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAg                                                                                                                  <  2:714333/140‑1 (MQ=255)
                      gCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAg                                                                                                                  <  2:78694/140‑1 (MQ=255)
                       cTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       >  2:727009/1‑134 (MQ=255)
                       cTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  1:727009/134‑1 (MQ=255)
                         aTCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGcac                                                                                                               <  1:503640/140‑1 (MQ=255)
                          tCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGcaca                                                                                                              >  2:923396/1‑140 (MQ=255)
                           cTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCacac                                                                                                             <  2:142910/140‑1 (MQ=255)
                           cTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCacac                                                                                                             <  2:228376/140‑1 (MQ=255)
                              gTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGca                                                                                                                >  1:364758/1‑134 (MQ=255)
                              gTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGca                                                                                                                <  2:364758/134‑1 (MQ=255)
                              gTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCAcaccac                                                                                                          >  1:1266668/1‑140 (MQ=255)
                               tCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       >  1:520747/1‑126 (MQ=255)
                               tCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCa                                                                                                                       <  2:520747/126‑1 (MQ=255)
                               tCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACcaca                                                                                                         >  1:92028/1‑140 (MQ=255)
                                        ggTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAaataa                                                                                                >  2:279615/1‑140 (MQ=255)
                                        ggTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAaataa                                                                                                >  2:536639/1‑140 (MQ=255)
                                              gTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGtt                                                                                          >  1:4293/1‑140 (MQ=255)
                                                    aTTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTaaa                                                                                    >  2:211109/1‑140 (MQ=255)
                                                    aTTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTaaa                                                                                    >  1:757892/1‑140 (MQ=255)
                                                    aTTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTaaa                                                                                    >  2:721720/1‑140 (MQ=255)
                                                    aTTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACATGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTaaa                                                                                    <  2:1179794/140‑1 (MQ=255)
                                                       aGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGa                                                                                 <  1:47362/140‑1 (MQ=255)
                                                       aGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGa                                                                                 <  1:923396/140‑1 (MQ=255)
                                                       aGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGa                                                                                 <  1:340104/140‑1 (MQ=255)
                                                       aGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGa                                                                                 <  1:1224294/140‑1 (MQ=255)
                                                       aGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGa                                                                                 <  1:211109/140‑1 (MQ=255)
                                                        gCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGAc                                                                                <  2:757892/140‑1 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               >  2:573657/1‑140 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               <  1:1131504/140‑1 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               >  1:1205004/1‑140 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               >  1:204163/1‑140 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               >  2:108501/1‑140 (MQ=255)
                                                         cTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGATGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACt                                                                               >  2:1131504/1‑140 (MQ=255)
                                                          ttACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTa                                                                              <  2:1205004/140‑1 (MQ=255)
                                                                 cATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATg                                                                       <  1:527489/140‑1 (MQ=255)
                                                                  aTGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACTACTAAAATATgg                                                                      >  2:227306/1‑140 (MQ=255)
                                                                  aTGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATgg                                                                      >  2:309405/1‑140 (MQ=255)
                                                                  aTGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATgg                                                                      <  2:648675/140‑1 (MQ=255)
                                                                   tGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGa                                                                     <  1:108501/140‑1 (MQ=255)
                                                                   tGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGa                                                                     <  2:152814/140‑1 (MQ=255)
                                                                   tGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGa                                                                     >  1:407554/1‑140 (MQ=255)
                                                                    ggggATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAg                                                                    <  1:573657/140‑1 (MQ=255)
                                                                     gggATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGa                                                                     <  2:1197888/138‑1 (MQ=255)
                                                                     gggATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGa                                                                     >  1:1197888/1‑138 (MQ=255)
                                                                     gggATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAgg                                                                   <  1:1257183/140‑1 (MQ=255)
                                                                     gggATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAgg                                                                   <  2:495599/140‑1 (MQ=255)
                                                                        aTTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGctc                                                                >  2:546581/1‑140 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  1:536639/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  2:646719/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  1:721720/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  2:65231/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  2:4293/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  2:1266668/140‑1 (MQ=255)
                                                                          ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtt                                                              <  2:92028/140‑1 (MQ=255)
                                                                            ttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttat                                                            >  1:880787/1‑140 (MQ=255)
                                                                                                gccagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtttt                                        >  1:671965/1‑140 (MQ=255)
                                                                                                        ttATAGGCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCtttt                                <  1:127748/140‑1 (MQ=255)
                                                                                                              gCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTaa                          <  2:671965/140‑1 (MQ=255)
                                                                                                              gCATGATGTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTaa                          <  1:1057625/140‑1 (MQ=255)
                                                                                                                    tgTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCtt                    <  2:204163/140‑1 (MQ=255)
                                                                                                                     gTTGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCttt                   <  1:984198/140‑1 (MQ=255)
                                                                                                                       tGATTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAt                 <  2:487537/140‑1 (MQ=255)
                                                                                                                         aTTTGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttt               >  2:350582/1‑140 (MQ=255)
                                                                                                                          tttGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttt              <  2:808733/140‑1 (MQ=255)
                                                                                                                           ttGTGCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttttt             <  1:279615/140‑1 (MQ=255)
                                                                                                                              tgCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTAt          <  1:281492/140‑1 (MQ=255)
                                                                                                                              tgCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTAt          >  1:969971/1‑140 (MQ=255)
                                                                                                                              tgCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTAt          <  2:793235/140‑1 (MQ=255)
                                                                                                                              tgCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTAt          >  2:842640/1‑140 (MQ=255)
                                                                                                                               gCCGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATg         >  1:399780/1‑140 (MQ=255)
                                                                                                                                ccGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttttt            <  2:412806/136‑1 (MQ=255)
                                                                                                                                ccGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttttt            >  1:412806/1‑136 (MQ=255)
                                                                                                                                 cGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTa       <  1:284993/140‑1 (MQ=255)
                                                                                                                                 cGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTa       <  1:546581/140‑1 (MQ=255)
                                                                                                                                 cGGTGTGTCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTa       <  2:25686/140‑1 (MQ=255)
                                                                                                                                      tgtCGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCtt  >  1:835967/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TATTCCTACAGGGATTGCTTATGCTATCTGGTCAGGAGTCGGTATTGTCCTGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTT  >  NC_000913/568473‑568746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑