Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,789	A→G	R399R (CGT→CGC)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,789	0	A	G	100.0%	279.2 / NA	74	R399R (CGT→CGC)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/32);  total (42/32)

GTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGACGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTAACCACT  >  NC_000913/987654‑987924                                                                                                                                        |                                                                                                                                        gTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGtt                                                                                                                                     <  2:237628/140‑1 (MQ=255)    aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGttctt                                                                                                                                  <  2:1032955/140‑1 (MQ=255)    aGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGttctt                                                                                                                                  >  2:980729/1‑140 (MQ=255)      cAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCa                                                                                                                                >  1:735056/1‑140 (MQ=255)       aaTCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCAc                                                                                                                               >  1:158119/1‑140 (MQ=255)        aTCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACg                                                                                                                              >  2:27435/1‑140 (MQ=255)          cAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCt                                                                                                                            <  1:27435/140‑1 (MQ=255)             aCGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTggg                                                                                                                         <  2:584469/140‑1 (MQ=255)               gTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAg                                                                                                                       <  2:1035317/140‑1 (MQ=255)                ttCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGc                                                                                                                      <  2:228871/140‑1 (MQ=255)                       ccAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAAt                                                                                                               >  1:804628/1‑140 (MQ=255)                         aaGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGa                                                                                                             >  2:721672/1‑140 (MQ=255)                                 aaCCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCg                                                                                                     >  2:778691/1‑140 (MQ=255)                                      gAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGcc                                                                                                <  1:778691/140‑1 (MQ=255)                                             gAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGcc                                                                                         <  2:804628/140‑1 (MQ=255)                                              aaCAGTACCGTAGCGACGCAGATCGCTATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGcc                                                                                                >  1:915104/1‑132 (MQ=255)                                              aaCAGTACCGTAGCGACGCAGATCGCTATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGcc                                                                                                <  2:915104/132‑1 (MQ=255)                                                        tAGCGACGCAGATCGCGATACCGCCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCAt                                                                              <  1:896208/140‑1 (MQ=255)                                                        tAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCAt                                                                              >  2:955534/1‑140 (MQ=255)                                                        tAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCAt                                                                              >  1:563833/1‑140 (MQ=255)                                                           cGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGc                                                                           <  1:721672/140‑1 (MQ=255)                                                             aCGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCg                                                                         >  1:94708/1‑140 (MQ=255)                                                             aCGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCg                                                                         >  1:23288/1‑140 (MQ=255)                                                             aCGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCg                                                                         >  1:657740/1‑140 (MQ=255)                                                                        gATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTa                                                              <  1:955534/140‑1 (MQ=255)                                                                           accaccAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCg                                                           >  1:782089/1‑140 (MQ=255)                                                                            ccaccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGt                                                          <  1:950130/140‑1 (MQ=255)                                                                             caccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACACTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTc                                                         <  1:1229118/140‑1 (MQ=255)                                                                               ccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCtt                                                       >  2:819887/1‑140 (MQ=255)                                                                               ccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCtt                                                       >  2:1235123/1‑140 (MQ=255)                                                                               ccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCtt                                                       >  1:27991/1‑140 (MQ=255)                                                                               ccaGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGAAACGGTTTTACCGTCtt                                                       >  2:381891/1‑140 (MQ=255)                                                                                  gTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGt                                                    >  1:349243/1‑140 (MQ=255)                                                                                   tAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGtt                                                   <  2:782089/140‑1 (MQ=255)                                                                                   tAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGtt                                                   <  2:735056/140‑1 (MQ=255)                                                                                       cttcttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAgg                                               <  1:819887/140‑1 (MQ=255)                                                                                       cttcttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAgg                                               <  1:42913/140‑1 (MQ=255)                                                                                       cttcttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAgg                                               <  1:1235123/140‑1 (MQ=255)                                                                                          cttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAgg                                               <  1:742411/137‑1 (MQ=255)                                                                                          cttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAgg                                               >  2:742411/1‑137 (MQ=255)                                                                                          cttTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGgcgc                                            >  2:533928/1‑140 (MQ=255)                                                                                           tttATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCa                                           <  1:298510/140‑1 (MQ=255)                                                                                             tATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCata                                         >  2:825511/1‑140 (MQ=255)                                                                                             tATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCata                                         >  2:181053/1‑140 (MQ=255)                                                                                             tATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGC‑Catat                                        >  2:956626/1‑140 (MQ=255)                                                                                                tCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAg                                      >  2:168436/1‑140 (MQ=255)                                                                                                   ggCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAAc                                   <  2:349243/140‑1 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGgc                                              <  1:1156865/128‑1 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGgc                                              >  2:1156865/1‑128 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACg                                  >  1:437454/1‑140 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACg                                  >  1:113914/1‑140 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACg                                  >  2:495748/1‑140 (MQ=255)                                                                                                    gCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACg                                  <  2:158119/140‑1 (MQ=255)                                                                                                       cATTTCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCtt                               >  1:1201414/1‑140 (MQ=255)                                                                                                         tttCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTa                             >  2:668023/1‑140 (MQ=255)                                                                                                           tCCAGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAat                           <  2:563833/140‑1 (MQ=255)                                                                                                              aGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAAc                                   >  1:938592/1‑129 (MQ=255)                                                                                                              aGCATACGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAAc                                   <  2:938592/129‑1 (MQ=255)                                                                                                                   aCGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCg                   >  2:1011876/1‑140 (MQ=255)                                                                                                                    cGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAat                           >  2:728314/1‑131 (MQ=255)                                                                                                                    cGCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAat                           <  1:728314/131‑1 (MQ=255)                                                                                                                     gCTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGa                 >  1:998874/1‑140 (MQ=255)                                                                                                                      cTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGAt                <  1:533928/140‑1 (MQ=255)                                                                                                                      cTCGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGAt                <  1:495748/140‑1 (MQ=255)                                                                                                                        cGTCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAg              >  2:822440/1‑140 (MQ=255)                                                                                                                          tCCAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGtt            >  1:163042/1‑140 (MQ=255)                                                                                                                           ccAGCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGttt           <  2:998874/140‑1 (MQ=255)                                                                                                                              gCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTa        <  2:1263030/140‑1 (MQ=255)                                                                                                                              gCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTa        >  2:1013935/1‑140 (MQ=255)                                                                                                                              gCACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTa        <  1:1123169/140‑1 (MQ=255)                                                                                                                               cACGTCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTaa       >  1:709751/1‑140 (MQ=255)                                                                                                                                   tCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTAACCAc   >  1:795567/1‑140 (MQ=255)                                                                                                                                   tCCAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTAACCAc   >  1:628569/1‑140 (MQ=255)                                                                                                                                    ccAGGCGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTAACCACt  >  2:311969/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACCGTAGCGACGCAGATCGCGATACCACCAGTAATCTTCTTTATTCAGGCCCATTTCCAGCATACGCTCGTCCAGCACGTCCAGACGTTCTTCACGCTGGGAGCCACCAATGATCTCACCGATGCCCGGAGCCAGAACGTCCATAGCCGCAACGGTTTTACCGTCTTCGTTAAGGCGCATATAGAACGCTTTAATATCTTTCGGATAGTTTTTAACCACT  >  NC_000913/987654‑987924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑