| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,070,462 | T→C | K165R (AAG→AGG) | rutE ← | putative malonic semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,070,462 | 0 | T | C | 98.3% | 212.9 / ‑4.3 | 59 | K165R (AAG→AGG) | rutE | putative malonic semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (32/26); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01 | |||||||||||
CGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCGAAAC > NC_000913/1070326‑1070593 | cgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCa > 1:1018599/1‑140 (MQ=255)cgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCa > 1:1185279/1‑140 (MQ=255) gttgATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGcc < 2:1018599/140‑1 (MQ=255) gACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAGAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTaaaa > 2:1099603/1‑140 (MQ=255) gACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTaaaa < 2:509843/140‑1 (MQ=255) gACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTaaaa < 1:281631/140‑1 (MQ=255) gTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGcg < 1:1099603/140‑1 (MQ=255) cATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGcgtcg < 1:188917/140‑1 (MQ=255) tAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCgtcgtc < 1:375509/140‑1 (MQ=255) gCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACgtg > 2:828672/1‑140 (MQ=255) gCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACgtg > 1:523716/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc > 1:411424/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc > 2:262436/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc > 2:34269/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc > 2:1063088/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc > 1:1208026/1‑140 (MQ=255) cccGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTAAAAGc > 1:359035/1‑140 (MQ=255) cGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGccc > 2:304811/1‑140 (MQ=255) cGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGccc > 1:356247/1‑140 (MQ=255) cGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAACCTTCCTGCTCTCGCCATAGCCCATCTTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACCTATTGACGGTCAAAGccc > 2:983232/1‑140 (MQ=255) cttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCGATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCGCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCggg < 1:983232/140‑1 (MQ=255) cttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCggg > 1:404005/1‑140 (MQ=255) cttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCggg < 2:404005/140‑1 (MQ=255) ttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt > 1:438570/1‑135 (MQ=255) ttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt < 2:438570/135‑1 (MQ=255) ttcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGc < 2:1226273/140‑1 (MQ=255) tcAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGcc < 2:1185279/140‑1 (MQ=255) cAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCg < 2:1024904/140‑1 (MQ=255) cAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCa > 2:896501/1‑140 (MQ=255) aGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAg > 2:631116/1‑140 (MQ=255) ggCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTAAAAGCCCGACATCGGGCCGGTATCCAGTCCCAg < 2:359035/140‑1 (MQ=255) gCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTAt < 2:131929/129‑1 (MQ=255) gCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTAt > 1:131929/1‑129 (MQ=255) gCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAgc > 2:927642/1‑140 (MQ=255) gCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAgc > 2:657384/1‑140 (MQ=255) cgcgcATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCg > 1:1029734/1‑140 (MQ=255) tAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGc < 2:523716/140‑1 (MQ=255) tAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGc < 2:584524/140‑1 (MQ=255) tAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGc < 2:1029734/140‑1 (MQ=255) tAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGc < 1:631116/140‑1 (MQ=255) tAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGc < 2:128912/140‑1 (MQ=255) aaGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGcga < 1:927642/140‑1 (MQ=255) gCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGcgacg < 2:498112/140‑1 (MQ=255) ctgctATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAg > 1:1263077/1‑140 (MQ=255) ctgctATCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAg > 1:734512/1‑140 (MQ=255) tCGCCATAGCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGgc > 2:803347/1‑140 (MQ=255) gCCGATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCa < 1:744064/140‑1 (MQ=255) gATATTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATgg < 1:262436/140‑1 (MQ=255) atatTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGa > 1:1170127/1‑140 (MQ=255) atatTAATCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGa > 1:1067948/1‑140 (MQ=255) aTCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGtt > 2:348671/1‑140 (MQ=255) aTCAGCAGATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGtt > 2:698966/1‑140 (MQ=255) agcagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCg > 1:1216384/1‑140 (MQ=255) agcagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCg < 1:348671/140‑1 (MQ=255) cagATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCGaa > 2:96917/1‑140 (MQ=255) cagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGaa < 1:245218/130‑1 (MQ=255) cagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGaa > 2:245218/1‑130 (MQ=255) cagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTg > 2:636597/1‑133 (MQ=255) cagATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTg < 1:636597/133‑1 (MQ=255) gATTGCTCCTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCGAAAc > 1:10769/1‑140 (MQ=255) | CGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCCCGGCAGGCGACGATCAGATAGGCCGCCTGCATGGAACTGTTGCGAAAC > NC_000913/1070326‑1070593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |