Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,813	A→G	C239C (TGT→TGC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,813	0	A	G	100.0%	153.0 / NA	46	C239C (TGT→TGC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

TGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTG  >  NC_000913/82677‑82945                                                                                                                                         |                                                                                                                                     tgtttgCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAg                                                                                                                                   >  1:999480/1‑140 (MQ=255)             tCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGGGCAGTTTCCGGCACGc                                                                                                                       >  2:1007534/1‑140 (MQ=255)                 cATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGc                                                                                                                   <  1:891441/140‑1 (MQ=255)                 cATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGc                                                                                                                   <  2:675008/140‑1 (MQ=255)                               ggCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCAtt                                                                                                     <  1:86069/140‑1 (MQ=255)                                gCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTGAGAATATCCTTACGAACCTTGATCGCCATGATGACTTCTTTCAGGGAGCAGTTTCCGGCACGCTCCCCGATCCCGGTCAttt                                                                                                    >  2:783099/1‑139 (MQ=255)                                gCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTg                                                                                                    >  1:518524/1‑140 (MQ=255)                                gCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTg                                                                                                    <  1:269182/140‑1 (MQ=255)                                gCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTg                                                                                                    >  2:675247/1‑140 (MQ=255)                                              tggTGCGCCATATCTCCTGGTTATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTgg                                                                                      >  1:135118/1‑140 (MQ=255)                                              tggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTgg                                                                                      >  2:567018/1‑140 (MQ=255)                                                gTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCg                                                                                    >  1:715085/1‑140 (MQ=255)                                                 tGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCt                                                                                               >  1:433423/1‑128 (MQ=255)                                                 tGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCt                                                                                               <  2:433423/128‑1 (MQ=255)                                                    gcCATATCTCCTGGTTATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCa                                                                                <  2:135118/140‑1 (MQ=255)                                                             ccTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGt                                                                       >  1:73109/1‑140 (MQ=255)                                                             ccTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCCGGCGGGCACCGGCATgg                                                                       >  2:160122/1‑139 (MQ=255)                                                                ggTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTAcc                                                                    >  2:120573/1‑140 (MQ=255)                                                                 gTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTAccg                                                                   <  2:495180/140‑1 (MQ=255)                                                                  tGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTAccgc                                                                  >  2:1010138/1‑140 (MQ=255)                                                                  tGATTAATGGCGGGGTGGACGTTGAGGATATCCTTTCGGAATTTGTTCGCCATGATGGCTTCTTCCAGGGAGCAGTTTTCCGCACGCTCGGCGGTCCCGTTCATTGTGCCTTCCACCTGGCGTGCACCGGCATGTAccgc                                                                  >  2:417836/1‑140 (MQ=255)                                                                    aTTAATGGCGGTGTGGACGTTGAGAATATTCTTACGAACTTTGATCGCCATGATGACTTTTTTCAGGGGGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTAccgccc                                                                >  2:566472/1‑139 (MQ=255)                                                                    aTTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTAccgccc                                                                >  1:5059/1‑139 (MQ=255)                                                                      tAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCcccgcc                                                              >  2:869968/1‑140 (MQ=255)                                                                         tGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGt                                                           <  1:567018/140‑1 (MQ=255)                                                                              gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAAGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAggt                                                      >  1:887023/1‑138 (MQ=255)                                                                               tgtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGttt                                                     >  1:724498/1‑140 (MQ=255)                                                                                      gTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCg                                              >  2:653295/1‑140 (MQ=255)                                                                                        tGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGcc                                            <  1:653295/140‑1 (MQ=255)                                                                                         gagaATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCa                                           >  1:155729/1‑140 (MQ=255)                                                                                         gagaATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCa                                           >  1:474820/1‑140 (MQ=255)                                                                                          agaATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAg                                          <  2:73109/140‑1 (MQ=255)                                                                                             atatCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGcc                                       >  2:515723/1‑140 (MQ=255)                                                                                                tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCaa                                    <  1:1010138/140‑1 (MQ=255)                                                                                                tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCaa                                    >  1:200388/1‑140 (MQ=255)                                                                                                 ccTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCaaa                                   <  2:474820/140‑1 (MQ=255)                                                                                                 ccTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCaaa                                   <  1:741912/140‑1 (MQ=255)                                                                                                  cTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAAt                                  <  1:754705/140‑1 (MQ=255)                                                                                                  cTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAAt                                  <  1:1252386/140‑1 (MQ=255)                                                                                                    tACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAAtcg                                >  2:1065796/1‑140 (MQ=255)                                                                                                       gAACTTTGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAAtcgtcg                             <  1:470085/140‑1 (MQ=255)                                                                                                            ttGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACTGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGt                        <  1:675247/140‑1 (MQ=255)                                                                                                            ttGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGt                        <  1:120573/140‑1 (MQ=255)                                                                                                             tGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTa                       >  1:683331/1‑140 (MQ=255)                                                                                                             tGATCGCCATGATGACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTa                       >  1:1179390/1‑140 (MQ=255)                                                                                                                     atgatgACTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGGCGTGGGTATGTACGga               >  1:879310/1‑140 (MQ=255)                                                                                                                         tgaCTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATa           <  2:518524/140‑1 (MQ=255)                                                                                                                           aCTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACg                 <  2:1067193/132‑1 (MQ=255)                                                                                                                           aCTTCTTCCAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACg                 >  1:1067193/1‑132 (MQ=255)                                                                                                                                  ccAGGGAGCAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTg  >  2:565193/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTG  >  NC_000913/82677‑82945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑