Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,285	A→G	G176G (GGT→GGC)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,285	0	A	G	100.0%	186.1 / NA	50	G176G (GGT→GGC)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/17);  total (33/17)

GGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAGCG  >  NC_000913/1257163‑1257415                                                                                                                           |                                                                                                                                   gggTTGTTTTCAAAACCGTTTTCGTTGACGTTGGCGATGTAAATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:1031315/1‑140 (MQ=255) gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:987462/1‑140 (MQ=255) gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:982279/1‑140 (MQ=255) gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:847345/1‑140 (MQ=255) gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:205911/1‑140 (MQ=255) gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:1079483/1‑140 (MQ=255)      gttTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggca                                                                                                              >  1:806408/1‑140 (MQ=255)      gttTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggca                                                                                                              >  1:186592/1‑140 (MQ=255)       ttttCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggcag                                                                                                             >  2:618075/1‑140 (MQ=255)           cAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  2:806408/140‑1 (MQ=255)           cAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  1:496727/140‑1 (MQ=255)              aaCCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAtttt                                                                                                      >  1:673868/1‑140 (MQ=255)                   tCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         >  1:426257/1‑132 (MQ=255)                   tCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  2:426257/132‑1 (MQ=255)                    cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                <  1:1262672/140‑1 (MQ=255)                    cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                <  2:1230176/140‑1 (MQ=255)                    cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                >  2:822389/1‑140 (MQ=255)                            aCGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcag                                                                                        <  2:186592/140‑1 (MQ=255)                                  gCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagctcagc                                                                                  >  1:426661/1‑140 (MQ=255)                                   cGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagctcagct                                                                                 >  2:616646/1‑140 (MQ=255)                                             tgttgGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCtt                                                                       >  2:453316/1‑140 (MQ=255)                                                tgGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTAt                                                                    >  1:335168/1‑140 (MQ=255)                                                 ggTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAACTCAGCTTTCGCGTCTTTATc                                                                   >  1:1013259/1‑140 (MQ=255)                                                  gTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTTTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCg                                                                  >  2:1095049/1‑140 (MQ=255)                                                  gTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCg                                                                  >  1:339102/1‑140 (MQ=255)                                                    ttCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGcc                                                                >  2:934963/1‑140 (MQ=255)                                                         cGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCtt                                                           <  2:673868/140‑1 (MQ=255)                                                         cGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCtt                                                           <  2:335168/140‑1 (MQ=255)                                                           tCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTg                                                         >  1:1024460/1‑140 (MQ=255)                                                            cAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTgg                                                        >  1:425838/1‑140 (MQ=255)                                                             aGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGc                                                       >  1:421191/1‑140 (MQ=255)                                                              ggAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCt                                                      <  2:1024460/140‑1 (MQ=255)                                                                   cTCAGGTAAAGAATAACCGCTTTCTCTTCAGGGCTTAAATCCAGCGCGCGCAGGATGCCTGTGTTTTCCAAGTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTttctt                                                 <  2:243547/140‑1 (MQ=255)                                                                         tAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTAc                                           >  1:787476/1‑140 (MQ=255)                                                                           aCGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTAcgc                                         >  2:295569/1‑140 (MQ=255)                                                                             gAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGa                                       >  2:853182/1‑140 (MQ=255)                                                                                tAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGa                                    >  1:296466/1‑140 (MQ=255)                                                                                       tttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAAt                                  <  2:970948/135‑1 (MQ=255)                                                                                       tttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAAt                                  >  1:970948/1‑135 (MQ=255)                                                                                        ttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACg                            <  1:1131754/140‑1 (MQ=255)                                                                                                aGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAgg                    >  2:1162985/1‑140 (MQ=255)                                                                                                  cgcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtg                  >  2:322935/1‑140 (MQ=255)                                                                                                   gcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtgt                 <  1:1162985/140‑1 (MQ=255)                                                                                                   gcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtgt                 <  1:1207001/140‑1 (MQ=255)                                                                                                         aTCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGt           <  1:205911/140‑1 (MQ=255)                                                                                                              gcgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGcc      <  1:1095049/140‑1 (MQ=255)                                                                                                              gcgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGcc      <  1:618075/140‑1 (MQ=255)                                                                                                               cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTTGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  1:505371/1‑140 (MQ=255)                                                                                                               cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGTGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  2:1260098/1‑140 (MQ=255)                                                                                                               cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  1:23397/1‑140 (MQ=255)                                                                                                               cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCACCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  2:180470/1‑140 (MQ=255)                                                                                                                  gcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGTGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAgcg  <  1:1260098/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                   GGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAGCG  >  NC_000913/1257163‑1257415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑