Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,144	A→G	L321L (CTA→CTG)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,144	0	A	G	100.0%	284.4 / NA	75	L321L (CTA→CTG)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/37);  total (38/37)

ATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAA  >  NC_000913/1302007‑1302273                                                                                                                                          |                                                                                                                                  aTCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTggg                                                                                                                                 >  2:738463/1‑140 (MQ=255)  tCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGt                                                                                                                                >  1:671294/1‑140 (MQ=255)   cGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTTTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTa                                                                                                                               <  2:202893/140‑1 (MQ=255)   cGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTa                                                                                                                               <  1:403581/140‑1 (MQ=255)      aTTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTggg                                                                                                                                 >  2:480262/1‑135 (MQ=255)        tGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGAc                                                                                                                          <  2:102530/140‑1 (MQ=255)          ttCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACcg                                                                                                                        >  1:457088/1‑140 (MQ=255)               gAAGCTAAAAAAAGAGACCCCGGCCGAAGTTCACGTTGATCAATACCTGTGCACTTATTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGata                                                                                                                   <  2:457088/140‑1 (MQ=255)                aaGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGatat                                                                                                                  >  1:1176575/1‑140 (MQ=255)                   cTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCAt                                                                                                               <  2:1006330/140‑1 (MQ=255)                   cTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCAt                                                                                                               <  1:944386/140‑1 (MQ=255)                   cTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCAt                                                                                                               <  1:176346/140‑1 (MQ=255)                   cTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCAt                                                                                                               <  1:738463/140‑1 (MQ=255)                    tGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCAtt                                                                                                              >  2:413599/1‑140 (MQ=255)                         aaaGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTtaa                                                                                                         <  2:294973/140‑1 (MQ=255)                                        gAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCa                                                                                          <  2:106749/140‑1 (MQ=255)                                         aaGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAg                                                                                         <  1:1203957/140‑1 (MQ=255)                                          aGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAgg                                                                                        >  2:1024212/1‑140 (MQ=255)                                                  ttGATCCATCCCTGTGCACTTACTATTACAAAATAAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATg                                                                                <  1:453677/140‑1 (MQ=255)                                                    gATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                                                              <  1:757817/140‑1 (MQ=255)                                                        cATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                                                          <  2:52482/140‑1 (MQ=255)                                                        cATACCTGTGCACTTACTATTACGAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                                                          <  2:997234/140‑1 (MQ=255)                                                          tcccggtgCACTTACTATTACAAAATTAACACCCAAAAACCGCCATTCAACGATGTGCGTGGGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa                                                                        <  1:218881/135‑1 (MQ=255)                                                          tACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa                                                                        >  2:1059060/1‑140 (MQ=255)                                                           aCCTGTGCACTTACTATTACGAAATTAATAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAt                                                                       >  2:172788/1‑140 (MQ=255)                                                               gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                                                   >  2:729935/1‑140 (MQ=255)                                                               gtgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                                                                   >  1:917772/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAATATGCCCGCCTATGGTTa                                                                  <  1:402122/140‑1 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                                                          <  2:1201805/132‑1 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                                                          >  1:1201805/1‑132 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  1:502724/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  1:942822/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  2:1017727/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  2:877850/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  1:770169/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  >  1:32095/1‑140 (MQ=255)                                                                tgCACTTACTATTACGAAATAAACACCAAAAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTa                                                                  <  2:1048884/140‑1 (MQ=255)                                                                 gCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtctg                                                                 >  1:1233693/1‑137 (MQ=255)                                                                    cTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                                                              <  1:413599/140‑1 (MQ=255)                                                                     ttACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTc                                                             >  1:1183111/1‑140 (MQ=255)                                                                      tACTTTTACAAAATAAACAACAAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGAACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc                                                            <  2:1176575/140‑1 (MQ=255)                                                                      tACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc                                                            <  1:729935/140‑1 (MQ=255)                                                                       tttATTCCAAAATAAACACCAAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCCTTGAAACTGGTTATGGACCGCAATATCATTGTTAATAAAGTGAAAGCGAAGGGAAACAGGCCCGCCTATGGTTACACTCCa                                                           <  2:1183111/138‑1 (MQ=255)                                                                        ttATTACGAAATTAACAACAAAAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc                                                          <  2:32095/139‑1 (MQ=255)                                                                        cttttgcGAAATAAACAACCAGAAGCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGCCCGCAATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACCTGCCCGCCTATGGTTACACTCCAc                                                          <  2:138107/134‑1 (MQ=255)                                                                          aTTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg                                                        >  1:126896/1‑140 (MQ=255)                                                                          aTTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg                                                        >  2:541111/1‑140 (MQ=255)                                                                                 aaTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTg                                                 >  2:226178/1‑140 (MQ=255)                                                                                 aaTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTg                                                 >  1:848168/1‑140 (MQ=255)                                                                                   ttAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                                           >  2:220498/1‑128 (MQ=255)                                                                                   ttAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCa                                                           <  1:220498/128‑1 (MQ=255)                                                                                            aGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCaaaa                                      <  1:1059060/140‑1 (MQ=255)                                                                                             gAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAAt                                     >  2:108084/1‑140 (MQ=255)                                                                                                aCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGa                                  <  2:848168/140‑1 (MQ=255)                                                                                                      aTTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGc                            <  2:917772/140‑1 (MQ=255)                                                                                                               tgtgcgtgtgcgTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGt                   >  1:93209/1‑140 (MQ=255)                                                                                                                 tgcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGttt                 >  2:907624/1‑140 (MQ=255)                                                                                                                 tgcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGttt                 >  2:509146/1‑140 (MQ=255)                                                                                                                 tgcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGttt                 >  1:720699/1‑140 (MQ=255)                                                                                                                 tgcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGCTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGa                        >  2:1179464/1‑133 (MQ=255)                                                                                                                 tgcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGCTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGa                        <  1:1179464/133‑1 (MQ=255)                                                                                                                  gcgtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTg                >  1:34217/1‑140 (MQ=255)                                                                                                                    gtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGc              <  1:385049/140‑1 (MQ=255)                                                                                                                    gtgtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGc              >  1:1091629/1‑140 (MQ=255)                                                                                                                      gtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTg            <  1:541111/140‑1 (MQ=255)                                                                                                                      gtgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTg            >  1:78226/1‑140 (MQ=255)                                                                                                                       tgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTg            >  1:602052/1‑139 (MQ=255)                                                                                                                       tgcgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTg            <  2:602052/139‑1 (MQ=255)                                                                                                                         cgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAg         >  2:8460/1‑140 (MQ=255)                                                                                                                         cgtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAg         >  2:1121630/1‑140 (MQ=255)                                                                                                                          gtACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGc        <  1:1024212/140‑1 (MQ=255)                                                                                                                           tACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGcc       <  1:960302/140‑1 (MQ=255)                                                                                                                              cGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAgg    <  1:907624/140‑1 (MQ=255)                                                                                                                                cACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa  >  1:921768/1‑140 (MQ=255)                                                                                                                                cACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa  >  2:108756/1‑140 (MQ=255)                                                                                                                                cACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa  <  2:93209/140‑1 (MQ=255)                                                                                                                                 aCTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa  >  1:345317/1‑139 (MQ=255)                                                                                                                                 aCTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa  <  2:345317/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  ATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAA  >  NC_000913/1302007‑1302273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑