Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	198,447	T→C	S174P (TCA→CCA)	bamA →	outer membrane protein assembly factor BamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	198,447	0	T	C	100.0%	270.4 / NA	71	S174P (TCA→CCA)	bamA	outer membrane protein assembly factor BamA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/37);  total (34/37)

CATTGCCGATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACCAGAAACAGAAA  >  NC_000913/198314‑198581                                                                                                                                      |                                                                                                                                       caTTGCCGATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTg                                                                                                                                  >  2:170386/1‑140 (MQ=255) caTTGCCGATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTg                                                                                                                                  <  1:170386/140‑1 (MQ=255)        gATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCa                                                                                                                           >  2:370981/1‑140 (MQ=255)        gATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCa                                                                                                                           >  1:515171/1‑140 (MQ=255)         atatCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAg                                                                                                                          <  2:275605/140‑1 (MQ=255)           atCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCa                                                                                                                        >  1:849653/1‑140 (MQ=255)           atCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCa                                                                                                                        >  2:11560/1‑140 (MQ=255)               agaAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAAtt                                                                                                                    >  2:903819/1‑140 (MQ=255)                   aGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACa                                                                                                                <  1:1250334/140‑1 (MQ=255)                      tCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACAt                                                                                                               >  1:122171/1‑138 (MQ=255)                      tCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACAt                                                                                                               <  2:122171/138‑1 (MQ=255)                        tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACAttgtt                                                                                                           >  1:554430/1‑140 (MQ=255)                          gAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACa                                                                                                                <  2:808211/133‑1 (MQ=255)                          gAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACa                                                                                                                >  1:808211/1‑133 (MQ=255)                               cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAAtt                                                                                                                    >  2:1204157/1‑124 (MQ=255)                               cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAAtt                                                                                                                    <  1:1204157/124‑1 (MQ=255)                               cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                                                                                                    <  1:14955/140‑1 (MQ=255)                               cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                                                                                                    <  2:919972/140‑1 (MQ=255)                               cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                                                                                                    <  1:903819/140‑1 (MQ=255)                                 tCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                                                                                                  <  2:515171/140‑1 (MQ=255)                                  ctactaCAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTAACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATg                                                                                                 >  1:858425/1‑140 (MQ=255)                                         aGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcac                                                                                          >  1:1249800/1‑140 (MQ=255)                                          gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                                                                                                    <  2:577345/129‑1 (MQ=255)                                          gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                                                                                                    >  1:577345/1‑129 (MQ=255)                                          gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcacc                                                                                         >  2:1269449/1‑140 (MQ=255)                                          gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcacc                                                                                         >  1:1047831/1‑140 (MQ=255)                                           cGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                                                                                                  <  1:1145391/130‑1 (MQ=255)                                           cGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                                                                                                  >  2:1145391/1‑130 (MQ=255)                                           cGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcacca                                                                                        >  2:477644/1‑140 (MQ=255)                                             tCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCaccacc                                                                                      >  1:824545/1‑140 (MQ=255)                                             tCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCaccacc                                                                                      >  1:924948/1‑140 (MQ=255)                                              cGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAGATTAACATTGTTGGTAACCATGCTTTCACCACcg                                                                                     <  2:573296/140‑1 (MQ=255)                                               ggTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcg                                                                                     <  1:831736/139‑1 (MQ=255)                                               ggTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcg                                                                                     >  2:831736/1‑139 (MQ=255)                                                  aaaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacga                                                                                 <  2:849653/140‑1 (MQ=255)                                                       taGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGa                                                                            <  2:1249800/140‑1 (MQ=255)                                                         gcgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtc                                                                          >  2:602133/1‑140 (MQ=255)                                                          cgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtct                                                                         >  2:548970/1‑140 (MQ=255)                                                          cgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtct                                                                         >  2:750966/1‑140 (MQ=255)                                                          cgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtct                                                                         >  1:934274/1‑140 (MQ=255)                                                           gcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtctc                                                                        >  1:534442/1‑140 (MQ=255)                                                           gcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtctc                                                                        <  1:548970/140‑1 (MQ=255)                                                           gcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtctc                                                                        <  1:749376/140‑1 (MQ=255)                                                           gcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGAtctc                                                                        >  2:203417/1‑140 (MQ=255)                                                               gCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCAt                                                                    >  2:132626/1‑140 (MQ=255)                                                                cGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCAtt                                                                   >  2:436706/1‑140 (MQ=255)                                                                    aaaGCTGTCGTGACCCCGCTGCCGCGCAATCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCa                                                               <  1:469425/140‑1 (MQ=255)                                                                     aaGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCaa                                                              <  1:201744/140‑1 (MQ=255)                                                                               gACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACg                                                    <  1:477644/140‑1 (MQ=255)                                                                                  cccGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAg                                                 <  1:132626/140‑1 (MQ=255)                                                                                    cccTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTg                                               <  2:858425/138‑1 (MQ=255)                                                                                     gCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGc                                              >  2:1206361/1‑140 (MQ=255)                                                                                      cTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGcc                                             <  1:296456/140‑1 (MQ=255)                                                                                      cTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGcc                                             <  2:1056905/140‑1 (MQ=255)                                                                                              cAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGa                                     <  2:534442/140‑1 (MQ=255)                                                                                               aaCCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGaa                                    <  2:1261429/140‑1 (MQ=255)                                                                                                   gtgtTGACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTg                                >  1:1245915/1‑140 (MQ=255)                                                                                                        gACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAgg                           <  1:1269449/140‑1 (MQ=255)                                                                                                        gACCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAgg                           <  1:802216/140‑1 (MQ=255)                                                                                                         aCCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCgtgg                                         <  1:1246652/125‑1 (MQ=255)                                                                                                         aCCTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCgtgg                                         >  2:1246652/1‑125 (MQ=255)                                                                                                          ccTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCg                         <  2:924948/140‑1 (MQ=255)                                                                                                           cTAAAACTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGa                        <  2:1247651/140‑1 (MQ=255)                                                                                                              aaaCTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGtggtgg                                      >  2:54385/1‑123 (MQ=255)                                                                                                              aaaCTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGtggtgg                                      <  1:54385/123‑1 (MQ=255)                                                                                                              aaaCTGGTGTTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCg                     >  1:423193/1‑140 (MQ=255)                                                                                                                     tgtTCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATAc              <  1:602133/140‑1 (MQ=255)                                                                                                                       ttCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACca            <  2:1047831/140‑1 (MQ=255)                                                                                                                        tCCAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACcag           <  1:872786/140‑1 (MQ=255)                                                                                                                         ccAGGAAGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACcaga          <  2:1245915/140‑1 (MQ=255)                                                                                                                               aGGTGTGCCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACcagaaacaga    >  1:420455/1‑140 (MQ=255)                                                                                                                                 gtgtgcCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACcagaaacagaaa  >  2:893463/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CATTGCCGATATCGAGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGTGACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACCAGAAACAGAAA  >  NC_000913/198314‑198581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑