Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,708,901	A→T	R661R (CGA→CGT)	rsxC →	SoxR [2Fe‑2S] reducing system protein RsxC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,901	0	A	T	100.0%	61.0 / NA	22	R661R (CGA→CGT)	rsxC	SoxR [2Fe‑2S] reducing system protein RsxC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/11);  total (11/11)

TGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC  >  NC_000913/1708805‑1709035                                                                                                 |                                                                                                                                       tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCCAATGCGGAACCAGAAGAACAGGTCTATCCGCGAAAAGCCGCCCTCCAATCCGCTATTGCCATATCAAAAGCGCGCAAACGGGAAAAGCAACCGGCAAAAGCgacgc                                                                                             >  2:50827/1‑136 (MQ=2)        gcgcGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                      <  2:475071/137‑1 (MQ=11)                          cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa                                                                    >  2:732918/1‑140 (MQ=11)                                      gcggaaccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                        <  1:732918/134‑1 (MQ=255)                                          aaccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGcc                                                    >  2:928518/3‑139 (MQ=255)                                          aaccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGcc                                                    >  2:500890/3‑139 (MQ=255)                                                 agaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTg                                             >  2:772529/3‑140 (MQ=255)                                                   aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCa                                           <  1:202571/140‑1 (MQ=255)                                                      aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCAcgc                                        <  1:928518/140‑1 (MQ=255)                                                       ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCAcgcg                                       >  2:1020114/1‑140 (MQ=255)                                                        gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGGGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGcc                                                    <  1:920117/126‑2 (MQ=255)                                                        gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGGGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGcc                                                    >  2:920117/1‑125 (MQ=255)                                                          cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCa                                    >  1:649872/1‑140 (MQ=255)                                                             tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAg                                 >  2:105575/1‑140 (MQ=255)                                                                 cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACg                             <  2:235843/140‑1 (MQ=255)                                                                  gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGc                            >  1:1193055/1‑140 (MQ=255)                                                                                gAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCa              <  1:1020114/140‑1 (MQ=255)                                                                                 aaGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa             <  2:1193055/140‑1 (MQ=255)                                                                                  aGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc            >  2:430607/1‑140 (MQ=255)                                                                                       cTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt       <  1:772529/140‑1 (MQ=255)                                                                                        tATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTa      >  1:646049/1‑140 (MQ=255)                                                                                            gCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc  <  1:500890/140‑1 (MQ=255)                                                                                            gCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc  <  1:430607/140‑1 (MQ=255)                                                                                                 |                                                                                                                                       TGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC  >  NC_000913/1708805‑1709035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑