Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,763,098	A→G	R304R (CGT→CGC)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,763,098	0	A	G	100.0%	157.3 / NA	44	R304R (CGT→CGC)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

CGCCACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAG  >  NC_000913/1762969‑1763227                                                                                                                                  |                                                                                                                                  cgcCACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGa                                                                                                                         >  2:205763/1‑140 (MQ=255) cgcCACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGa                                                                                                                         >  1:972625/1‑140 (MQ=255)   ccACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACg                                                                                                                       <  1:1096973/140‑1 (MQ=255)   ccACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACg                                                                                                                       <  1:720779/140‑1 (MQ=255)   ccACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACg                                                                                                                       <  2:819158/140‑1 (MQ=255)     aCTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGaa                                                                                                                     <  2:916890/140‑1 (MQ=255)      cTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAg                                                                                                                    >  1:5452/1‑140 (MQ=255)              aaCTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAAt                                                                                                            >  2:802444/1‑140 (MQ=255)                cTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAc                                                                                                          <  1:205763/140‑1 (MQ=255)                   aCCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccgc                                                                                                       <  2:893464/140‑1 (MQ=255)                   aCCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccgc                                                                                                       >  2:63907/1‑140 (MQ=255)                   aCCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccgc                                                                                                       <  2:277983/140‑1 (MQ=255)                     cAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccgccg                                                                                                     >  1:525643/1‑140 (MQ=255)                      aaTGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCgg                                                                                                    <  2:253175/140‑1 (MQ=255)                       aTGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGgt                                                                                                   <  1:802444/140‑1 (MQ=255)                           aGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccg                                                                                                        >  1:239397/1‑131 (MQ=255)                           aGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATAccg                                                                                                        <  2:239397/131‑1 (MQ=255)                           aGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGtgttg                                                                                               >  1:642533/1‑140 (MQ=255)                             ttATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTgttgtt                                                                                             >  2:754894/1‑140 (MQ=255)                               aTCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTAt                                                                                           >  2:1019171/1‑140 (MQ=255)                                 cgccgcGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCg                                                                                         >  1:493813/1‑140 (MQ=255)                                  gccgcGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGc                                                                                        <  2:847104/140‑1 (MQ=255)                                    cgcgCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCa                                                                                      >  1:1018147/1‑140 (MQ=255)                                       gcAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGa                                                                                   >  1:770027/1‑140 (MQ=255)                                       gcAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGa                                                                                   <  2:770027/140‑1 (MQ=255)                                          aaaTGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAAc                                                                                <  1:191205/140‑1 (MQ=255)                                            aTGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCa                                                                              <  2:1017376/140‑1 (MQ=255)                                                 gCTGGGATCTTCCCCCGTAATCGCTGTCCCGGTTTCTGATGGCGTCCATGCCATTTTGTTGTTTGCGCTTCCGCACAAAGCGCGCTTGGTGGCGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGtttt                                                                         <  2:301928/140‑1 (MQ=255)                                                     ggATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTAc                                                                     >  1:603992/1‑140 (MQ=255)                                                        tatTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGt                                                                  >  2:1167149/1‑140 (MQ=255)                                                         atTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTg                                                                 <  1:428542/140‑1 (MQ=255)                                                           ttCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGa                                                               <  2:1048433/140‑1 (MQ=255)                                                           ttCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGa                                                               <  1:1167149/140‑1 (MQ=255)                                                                cGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATAtt                                                          >  2:1243469/1‑140 (MQ=255)                                                                 gTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATAttt                                                         <  1:734413/140‑1 (MQ=255)                                                                       gCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTc                                                   <  1:754894/140‑1 (MQ=255)                                                                        cTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCg                                                  <  1:1019171/140‑1 (MQ=255)                                                                         tGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCgg                                                 <  2:493813/140‑1 (MQ=255)                                                                           aCCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCg                                               <  2:603992/140‑1 (MQ=255)                                                                           aCCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCg                                               >  2:716422/1‑140 (MQ=255)                                                                           aCCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTGTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCGAGGAAACTAGTTTTGTACCGTGGAATATTTCACCgcggcg                                               >  2:1118572/1‑140 (MQ=255)                                                                                    cTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTGCCGTGGAATATTTCCCCTCGGCGTTTTTATgg                                      <  2:5452/140‑1 (MQ=255)                                                                                        ttGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatga                                  <  2:1018147/140‑1 (MQ=255)                                                                                           cGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGAtgatga                               >  1:185012/1‑140 (MQ=255)                                                                                               cATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTc                           >  1:1090473/1‑140 (MQ=255)                                                                                                                   gCCTTCGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACt       >  1:262308/1‑140 (MQ=255)                                                                                                                        cGCACAAAGCGCGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAg  >  1:1178929/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                  CGCCACTGAGTAAAACTCACCAATGGAGTTATCGCCGCGCAAAATGCAGCTGGGATATTTCCACGTAATCGCTGACCCGGTTTCTGATTGCGTCCATGACATTTTGCTGTTTTCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAG  >  NC_000913/1762969‑1763227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑