Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,952,700	T→C	intergenic (+39/‑2)	yecN → / → cmoA	putative inner membrane protein/carboxy‑S‑adenosyl‑L‑methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,952,700	0	T	C	100.0%	331.6 / NA	88	intergenic (+39/‑2)	yecN/cmoA	putative inner membrane protein/carboxy‑S‑adenosyl‑L‑methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/45);  total (43/45)

CGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATG  >  NC_000913/1952566‑1952836                                                                                                                                        |                                                                                                                                         cGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGt                                                                                                                                     <  2:360723/140‑1 (MQ=255)   ttCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGtct                                                                                                                                   <  2:1051877/140‑1 (MQ=255)           tGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGa                                                                                                                           <  2:673055/140‑1 (MQ=255)             aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGaca                                                                                                                         >  1:830599/1‑140 (MQ=255)               cgcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACg                                                                                                                       >  2:797207/1‑140 (MQ=255)                  cACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTa                                                                                                                    >  2:739953/1‑140 (MQ=255)                         tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTg                                                                                                             <  1:1068015/140‑1 (MQ=255)                          gtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGc                                                                                                            >  2:791818/1‑140 (MQ=255)                            gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                                                          >  1:31088/1‑140 (MQ=255)                             cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                                                         <  1:674674/140‑1 (MQ=255)                             cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                                                         >  2:698262/1‑140 (MQ=255)                               cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                                                                       >  1:968489/1‑140 (MQ=255)                               cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                                                                       >  1:761836/1‑140 (MQ=255)                                  ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                                                                    >  1:404623/1‑140 (MQ=255)                                  ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                                                                    >  2:155162/1‑140 (MQ=255)                                          ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                                                            <  1:698262/140‑1 (MQ=255)                                          ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                                                            <  2:310605/140‑1 (MQ=255)                                           gTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTgg                                                                                           <  2:224681/140‑1 (MQ=255)                                            tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                                                          >  2:1205514/1‑140 (MQ=255)                                            tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                                                          >  1:645850/1‑140 (MQ=255)                                            tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                                                          >  1:565176/1‑140 (MQ=255)                                               tGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGa                                                                                       >  1:995840/1‑140 (MQ=255)                                                ggCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGAc                                                                                      <  1:7056/140‑1 (MQ=255)                                                ggCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGAc                                                                                      <  1:1226989/140‑1 (MQ=255)                                                ggCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGAc                                                                                      <  1:445082/140‑1 (MQ=255)                                                  cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                                                                    >  2:338244/1‑140 (MQ=255)                                                  cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                                                                    <  1:1000117/140‑1 (MQ=255)                                                  cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                                                                    <  2:830599/140‑1 (MQ=255)                                                      tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAcc                                                                                >  1:1009121/1‑140 (MQ=255)                                                           ggTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtga                                                                           >  1:978839/1‑140 (MQ=255)                                                             tataTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgatg                                                                         >  2:556950/1‑140 (MQ=255)                                                               tatGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                                                                       <  2:493433/140‑1 (MQ=255)                                                               tatGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                                                                       <  2:1101644/140‑1 (MQ=255)                                                               tatGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                                                                       <  2:482819/140‑1 (MQ=255)                                                                 tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                                                                    <  1:782988/126‑1 (MQ=255)                                                                 tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                                                                    >  2:782988/1‑126 (MQ=255)                                                                 tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                                                                     >  2:649155/1‑140 (MQ=255)                                                                  gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg                                                                    >  2:978817/1‑140 (MQ=255)                                                                   cccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                                                                   >  1:1124519/1‑140 (MQ=255)                                                                      tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                                                                >  2:816123/1‑140 (MQ=255)                                                                      tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                                                                >  2:1045017/1‑140 (MQ=255)                                                                       gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                                                                   <  1:701788/136‑1 (MQ=255)                                                                       gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                                                                   >  2:701788/1‑136 (MQ=255)                                                                       gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                                                               >  1:121220/1‑140 (MQ=255)                                                                           gTTGGTTTTCTCCCTGTGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                                                           <  1:214613/140‑1 (MQ=255)                                                                           gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                                                           <  1:791818/140‑1 (MQ=255)                                                                           gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                                                           <  2:968489/140‑1 (MQ=255)                                                                           gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                                                           >  1:633080/1‑140 (MQ=255)                                                                            ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAg                                                          >  2:48362/1‑140 (MQ=255)                                                                            ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGAAGCTGAAg                                                          >  2:700971/1‑140 (MQ=255)                                                                              ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                                                        >  1:919723/1‑140 (MQ=255)                                                                              ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                                                        >  2:402594/1‑140 (MQ=255)                                                                              ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                                                        >  2:684677/1‑140 (MQ=255)                                                                                 tttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc                                                     <  2:978839/140‑1 (MQ=255)                                                                                   tctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc                                                   <  2:633080/140‑1 (MQ=255)                                                                                   tctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc                                                   <  2:31088/140‑1 (MQ=255)                                                                                    ctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCg                                                  >  2:1136816/1‑140 (MQ=255)                                                                                        cTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata                                              <  1:1221041/140‑1 (MQ=255)                                                                                        cTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata                                              <  1:739953/140‑1 (MQ=255)                                                                                         tGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGatat                                             >  2:1233191/1‑140 (MQ=255)                                                                                         tGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGatat                                             >  1:584994/1‑140 (MQ=255)                                                                                               aGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCa                                       <  1:816123/140‑1 (MQ=255)                                                                                               aGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCa                                       <  1:444558/140‑1 (MQ=255)                                                                                                gcgcACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAg                                      <  2:565176/140‑1 (MQ=255)                                                                                                 cgcACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGTCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGc                                     >  1:236090/1‑140 (MQ=255)                                                                                                 cgcACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGc                                     >  2:1146731/1‑140 (MQ=255)                                                                                                     caATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATTGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc                                                   <  1:266649/122‑1 (MQ=255)                                                                                                     caATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATTGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc                                                   >  2:266649/1‑122 (MQ=255)                                                                                                     caATACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTc                                 >  1:165664/1‑140 (MQ=255)                                                                                                        tACGCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGt                              >  1:1253651/1‑140 (MQ=255)                                                                                                           gCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGtt                             >  2:1146398/1‑137 (MQ=255)                                                                                                           gCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGtt                             <  1:1146398/138‑2 (MQ=255)                                                                                                           gCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTAcc                           <  1:338244/140‑1 (MQ=255)                                                                                                           gCCACTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTAcc                           <  1:1233191/140‑1 (MQ=255)                                                                                                              aCTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCgg                        <  1:1045017/140‑1 (MQ=255)                                                                                                              aCTTTCTTTTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCgg                        <  1:48362/140‑1 (MQ=255)                                                                                                               ctttctttTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGc                       <  2:485000/140‑1 (MQ=255)                                                                                                                tttctttTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTa                             >  1:233560/1‑133 (MQ=255)                                                                                                                tttctttTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTa                             <  2:233560/133‑1 (MQ=255)                                                                                                                tttctttTT‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCt                      >  2:715943/1‑140 (MQ=255)                                                                                                                    ttttt‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTc                  <  2:1253651/140‑1 (MQ=255)                                                                                                                    ttttt‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTc                  <  1:684677/140‑1 (MQ=255)                                                                                                                      ttt‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCa                <  2:404623/140‑1 (MQ=255)                                                                                                                       tt‑CCCGGATTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCaa               <  2:645850/140‑1 (MQ=255)                                                                                                                               aTTTTTACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATattatt        <  1:155162/140‑1 (MQ=255)                                                                                                                                 ttttACGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTc      <  1:402594/140‑1 (MQ=255)                                                                                                                                   ttACGCTATGTCTCACCGCGACACGCTATTTTCTGTCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCa    <  2:236090/140‑1 (MQ=255)                                                                                                                                     aCGCTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATg  <  1:797207/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTT‑CCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATG  >  NC_000913/1952566‑1952836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑