Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,971,142	G→A	A517V (GCC→GTC)	tar ←	methyl‑accepting chemotaxis protein Tar

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,971,142	0	G	A	100.0%	142.8 / NA	42	A517V (GCC→GTC)	tar	methyl‑accepting chemotaxis protein Tar
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/18);  total (24/18)

CGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGGCTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCAAT  >  NC_000913/1971014‑1971276                                                                                                                                 |                                                                                                                                       cgTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACgg                                                                                                                             >  2:386759/1‑140 (MQ=255)     aGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAAcg                                                                                                                         <  2:888344/140‑1 (MQ=255)     aGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAAcg                                                                                                                         <  1:36702/140‑1 (MQ=255)            aCTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAAc                                                                                                                  >  1:431091/1‑140 (MQ=255)               cGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGc                                                                                                               <  2:177935/140‑1 (MQ=255)                gTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCt                                                                                                              >  1:1167839/1‑140 (MQ=255)                gTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCt                                                                                                              >  2:1009797/1‑140 (MQ=255)                gTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCt                                                                                                              >  1:333687/1‑140 (MQ=255)                gTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCt                                                                                                              >  1:322064/1‑140 (MQ=255)                gTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCt                                                                                                              >  1:244526/1‑140 (MQ=255)                 tCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCtt                                                                                                             >  2:618054/1‑140 (MQ=255)                    aaaTGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCg                                                                                                          <  2:1167839/140‑1 (MQ=255)                       tGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTa                                                                                                       <  2:322064/140‑1 (MQ=255)                           tCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACg                                                                                                   >  1:157930/1‑140 (MQ=255)                                gTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCg                                                                                              <  2:482823/140‑1 (MQ=255)                                gTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCg                                                                                              <  1:618054/140‑1 (MQ=255)                                           gTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTc                                                                                               <  2:79496/128‑1 (MQ=255)                                           gTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTc                                                                                               >  1:79496/1‑128 (MQ=255)                                           gTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTcc                                                                                   >  1:654819/1‑140 (MQ=255)                                                         aGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAgcggcggc                                                                     >  1:1260068/1‑140 (MQ=255)                                                           tCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGcggcggcgg                                                                   <  2:654819/140‑1 (MQ=255)                                                                     gCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTc                                                         >  1:15771/1‑140 (MQ=255)                                                                             ttGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCa                                                 <  1:549208/140‑1 (MQ=255)                                                                               gCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGc                                               >  2:722236/1‑140 (MQ=255)                                                                                cTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCg                                              <  2:939133/140‑1 (MQ=255)                                                                                  cACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGAt                                            <  2:409088/140‑1 (MQ=255)                                                                                   aCTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATg                                           >  2:209315/1‑140 (MQ=255)                                                                                     tGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCg                                         <  1:386759/140‑1 (MQ=255)                                                                                       gCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGtt                                       <  1:404180/140‑1 (MQ=255)                                                                                               ggATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCg                               <  2:244526/140‑1 (MQ=255)                                                                                                     tgtTTGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGAcgc                         >  1:473717/1‑140 (MQ=255)                                                                                                       tttGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGa                       >  1:26979/1‑140 (MQ=255)                                                                                                       tttGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGa                       >  1:821915/1‑140 (MQ=255)                                                                                                         tGCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATc                     <  1:722236/140‑1 (MQ=255)                                                                                                          gCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATcc                    >  2:319163/1‑140 (MQ=255)                                                                                                          gCGGTTTATTGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATcc                    <  1:319163/140‑1 (MQ=255)                                                                                                                   tggtggGGGGGCTGACTGCCAGACGGAACGGGGAAACTGCTTGCTTTAAACGACTCGCTTGTTCTCCCAGCGCAGCGGCGGCGGCAGCTGATTCCTCCCCCAGCGATGCGTTCTGTTGCGTGACGCGATCC‑TTTCCCaaa          <  2:431091/140‑1 (MQ=255)                                                                                                                   tGGTGAGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGaa           >  1:270583/1‑140 (MQ=255)                                                                                                                        aGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCg       <  2:1115540/139‑1 (MQ=255)                                                                                                                        aGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCg       >  1:1115540/1‑139 (MQ=255)                                                                                                                        aGTGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGc      >  2:1110765/1‑140 (MQ=255)                                                                                                                          tGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCa    >  2:655604/1‑140 (MQ=255)                                                                                                                          tGGGCTGACTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCa    >  2:772436/1‑140 (MQ=255)                                                                                                                            ggctgactGCCGGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCAAt  >  1:783747/1‑140 (MQ=255)                                                                                                                            ggctgactGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCAAt  >  1:570137/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       CGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGTGAGTGGGCTGGCTGCCAGACGGAACGCGGAAACTGCTTGCGTTAAACGACTCGCCTGTTCTTCCAGCGCAGCGGCGGCGGCAGCTGATTCCTGCACCAGCGATGCGTTCTGTTGCGTGACGCGATCCATTTCCGAAACCGCCAAT  >  NC_000913/1971014‑1971276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑