Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,446	T→C	V120A (GTG→GCG)	fliN →	flagellar motor switch protein FliN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,446	0	T	C	100.0%	205.8 / NA	55	V120A (GTG→GCG)	fliN	flagellar motor switch protein FliN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/32);  total (23/32)

AAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTC  >  NC_000913/2021314‑2021578                                                                                                                                     |                                                                                                                                     aaGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATc                                                                                                                               >  2:84120/1‑140 (MQ=255) aaGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATc                                                                                                                               <  2:611287/140‑1 (MQ=255)    agCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGAt                                                                                                                                >  1:901910/1‑136 (MQ=255)    agCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGAt                                                                                                                                <  2:901910/136‑1 (MQ=255)    agCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCAcc                                                                                                                            <  2:1224382/140‑1 (MQ=255)    agCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCAcc                                                                                                                            <  1:742927/140‑1 (MQ=255)      cTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGa                                                                                                                          <  2:1224431/140‑1 (MQ=255)      cTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGa                                                                                                                          <  2:1074253/140‑1 (MQ=255)        gTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCAcc                                                                                                                            >  2:413683/1‑136 (MQ=255)        gTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCAcc                                                                                                                            <  1:413683/136‑1 (MQ=255)              tCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGa                                                                                                                          <  2:878999/132‑1 (MQ=255)              tCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGa                                                                                                                          >  1:878999/1‑132 (MQ=255)              tCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTa                                                                                                                  <  2:644134/140‑1 (MQ=255)                       aGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTg                                                                                                         <  1:345918/140‑1 (MQ=255)                         ggTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGGTATTTTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGGGGTCGTTGCCGATAAATATGGCGCGCGGGGGACCGGGATCATTTCTCCGTTTGAg                                                                                                       >  2:1190123/1‑140 (MQ=255)                              gtcgtGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGGTCACCGATATCATTTCTCCCGCTGAGCggaa                                                                                                  >  2:1248324/1‑137 (MQ=255)                                cgtGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATgc                                                                                                >  1:736253/1‑140 (MQ=255)                                  tGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGaa                                                                                                   >  1:160711/1‑135 (MQ=255)                                  tGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGaa                                                                                                   <  2:160711/135‑1 (MQ=255)                                      gcTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCgccgcc                                                                                          <  1:292445/140‑1 (MQ=255)                                      gcTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCgccgcc                                                                                          <  1:225979/140‑1 (MQ=255)                                            cGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGcc                                                                                    >  1:678078/1‑140 (MQ=255)                                                 tGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTTCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAg                                                                               <  1:1108391/140‑1 (MQ=255)                                                  ggcgggcgAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGt                                                                              <  2:678078/140‑1 (MQ=255)                                                  ggcgggcgAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGt                                                                              <  1:84120/140‑1 (MQ=255)                                                    cgggcgAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGtga                                                                            >  1:603501/1‑140 (MQ=255)                                                         gAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCCCCTGAGCCGTTAGTGATGAAt                                                                       >  2:1191668/1‑140 (MQ=255)                                                              aCTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCa                                                                  <  1:334207/140‑1 (MQ=255)                                                                tGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACg                                                                <  1:35175/140‑1 (MQ=255)                                                                     atTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACt                                                           <  1:560903/140‑1 (MQ=255)                                                                     atTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACt                                                           <  1:483011/140‑1 (MQ=255)                                                                       tCTGATCAACGGTTATTTAATCGCCCAGGGCGACGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGGTATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACtgt                                                         >  2:528017/1‑140 (MQ=255)                                                                       tCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACtgt                                                         >  2:420611/1‑140 (MQ=255)                                                                       tCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACtgt                                                         >  2:509092/1‑140 (MQ=255)                                                                       tCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACtgt                                                         >  2:852056/1‑140 (MQ=255)                                                                       tCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACtgt                                                         >  2:1267707/1‑140 (MQ=255)                                                                            tCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAAt                                                    >  1:958357/1‑140 (MQ=255)                                                                              aaCGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCa                                                                  <  2:1111493/124‑1 (MQ=255)                                                                              aaCGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCa                                                                  >  1:1111493/1‑124 (MQ=255)                                                                                cGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTc                                                >  1:8912/1‑140 (MQ=255)                                                                                aggTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTc                                                >  1:158199/2‑140 (MQ=255)                                                                                    tATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCcgcg                                            <  2:8912/140‑1 (MQ=255)                                                                                    tATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCcgcg                                            <  2:158199/140‑1 (MQ=255)                                                                                         aaTCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGt                                       <  1:1267707/140‑1 (MQ=255)                                                                                         aaTCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGt                                       <  1:852056/140‑1 (MQ=255)                                                                                               ccAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTctgc                                 >  1:1059008/1‑140 (MQ=255)                                                                                                     cGAAGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                           <  1:509092/140‑1 (MQ=255)                                                                                                        aGTGGTGGTCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCAct                        >  2:547623/1‑140 (MQ=255)                                                                                                             tggtCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgc                      <  1:884899/137‑1 (MQ=255)                                                                                                             tggtCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgc                      >  2:884899/1‑137 (MQ=255)                                                                                                             tggtCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACtgctgc                   >  2:510636/1‑140 (MQ=255)                                                                                                             tggtCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACtgctgc                   >  1:679873/1‑140 (MQ=255)                                                                                                             tggtCGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACtgctgc                   >  1:581195/1‑140 (MQ=255)                                                                                                                 cGTTGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGt               <  1:1190123/140‑1 (MQ=255)                                                                                                                    tGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAg            >  1:716573/1‑140 (MQ=255)                                                                                                                    tGCCGATAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAg            <  1:420611/140‑1 (MQ=255)                                                                                                                         aTAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAgcggcg       <  2:603501/140‑1 (MQ=255)                                                                                                                         aTAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAgcggcg       <  1:547623/140‑1 (MQ=255)                                                                                                                         aTAAATATGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAgcggcg       <  2:1059008/140‑1 (MQ=255)                                                                                                                              tatGGCGCGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTc  >  2:559273/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     AAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTC  >  NC_000913/2021314‑2021578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑