Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,622	C→T	noncoding (74/76 nt)	asnT →	tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,622	0	C	T	100.0%	168.3 / NA	50	noncoding (74/76 nt)	asnT	tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

GACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAT  >  NC_000913/2044484‑2044758                                                                                                                                           |                                                                                                                                         gACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGcc                                                                                                                                         >  1:1044935/1‑140 (MQ=255)   cTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCaa                                                                                                                                       >  2:1212800/1‑140 (MQ=255)    ttATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCaaa                                                                                                                                      >  1:378228/1‑140 (MQ=255)              tAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAAt                                                                                                                            >  1:501371/1‑140 (MQ=255)                      acagacaAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCtttt                                                                                                                    >  1:311328/1‑140 (MQ=255)                      acagacaAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCtttt                                                                                                                    >  1:157427/1‑140 (MQ=255)                          acaAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAg                                                                                                                >  2:271599/1‑140 (MQ=255)                           caAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAgc                                                                                                               >  2:1241771/1‑140 (MQ=255)                            aaGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAgcg                                                                                                              <  2:378228/140‑1 (MQ=255)                               gTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCaa                                                                                                           >  2:825854/1‑140 (MQ=255)                                    gCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCa                                                                                                      <  1:1241771/140‑1 (MQ=255)                                    gCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCa                                                                                                      <  2:731354/140‑1 (MQ=255)                                     cTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCAc                                                                                                     <  2:72992/140‑1 (MQ=255)                                         gTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGAc                                                                                                 <  2:157427/140‑1 (MQ=255)                                         gTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGAc                                                                                                 <  2:1190335/140‑1 (MQ=255)                                          tAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGAcc                                                                                                >  2:1232309/1‑140 (MQ=255)                                           aaTATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCt                                                                                               <  2:311328/140‑1 (MQ=255)                                              atTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAg                                                                                            <  1:1097936/140‑1 (MQ=255)                                                    cccGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAc                                                                                      >  1:830007/1‑140 (MQ=255)                                                             acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                                             >  2:1206623/1‑140 (MQ=255)                                                                  tCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACg                                                                        >  2:607641/1‑140 (MQ=255)                                                                  tCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACg                                                                        >  2:1160277/1‑140 (MQ=255)                                                                       tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                                                   >  1:1082159/1‑140 (MQ=255)                                                                       tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                                                   >  2:699524/1‑140 (MQ=255)                                                                           gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCggg                                                               >  1:308273/1‑140 (MQ=255)                                                                            ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGt                                                              >  1:44918/1‑140 (MQ=255)                                                                                gTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttttt                                                          <  2:830007/140‑1 (MQ=255)                                                                                 tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                                                         >  2:1146278/1‑140 (MQ=255)                                                                                        aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCtt                                                  >  2:839391/1‑140 (MQ=255)                                                                                        aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCtt                                                  <  2:1029846/140‑1 (MQ=255)                                                                                          cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGc                                                                  >  1:291329/1‑122 (MQ=255)                                                                                          cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGc                                                                  <  2:291329/122‑1 (MQ=255)                                                                                           ggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAg                                               <  1:839391/140‑1 (MQ=255)                                                                                             cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcg                                             >  2:158140/1‑140 (MQ=255)                                                                                                 cTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCt                                         <  2:1206635/140‑1 (MQ=255)                                                                                                  tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTc                                        <  2:896319/140‑1 (MQ=255)                                                                                                  tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTc                                        <  1:492462/140‑1 (MQ=255)                                                                                                   gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCg                                       <  1:1206623/140‑1 (MQ=255)                                                                                                     tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAc                                     >  2:1267823/1‑140 (MQ=255)                                                                                                     tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAc                                     <  1:1146278/140‑1 (MQ=255)                                                                                                              tGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGt                            >  1:1189859/1‑140 (MQ=255)                                                                                                                  aCTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAg                        >  1:856671/1‑140 (MQ=255)                                                                                                                         cGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGttt                 >  1:950873/1‑140 (MQ=255)                                                                                                                          gAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGtttt                >  2:928032/1‑140 (MQ=255)                                                                                                                           aGTCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg               <  1:1212800/140‑1 (MQ=255)                                                                                                                             tCCAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCt             >  1:223438/1‑140 (MQ=255)                                                                                                                              ccAGTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTa            >  1:958692/1‑140 (MQ=255)                                                                                                                                 gTCAGAGGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGa         <  1:25334/140‑1 (MQ=255)                                                                                                                                    agagGAGTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACg      <  2:371003/140‑1 (MQ=255)                                                                                                                                       ggagTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCa   <  1:607641/140‑1 (MQ=255)                                                                                                                                        gagTCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt  <  1:699524/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         GACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAT  >  NC_000913/2044484‑2044758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑