Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,079,953	C→G	M213I (ATG→ATC)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,079,953	0	C	G	100.0%	221.6 / NA	62	M213I (ATG→ATC)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (34/28);  total (34/28)

TGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGC  >  NC_000913/2079820‑2080082                                                                                                                                      |                                                                                                                                  tGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGGCCACGGATTTTc                                                                                                                             >  2:980552/1‑140 (MQ=255)   gggCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCAc                                                                                                                           >  1:324816/1‑140 (MQ=255)        cgTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCa                                                                                                                       <  1:1090214/139‑1 (MQ=255)        cgTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCa                                                                                                                       >  2:1090214/1‑139 (MQ=255)         gTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATc                                                                                                                     >  2:247188/1‑140 (MQ=255)             cccAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTc                                                                                                                 <  1:247188/140‑1 (MQ=255)                aGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAgg                                                                                                              <  1:1158604/140‑1 (MQ=255)                  tATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTATCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAg                                                                                                            <  2:750772/140‑1 (MQ=255)                       aTTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGttttt                                                                                                       >  2:20995/1‑140 (MQ=255)                       aTTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGttt                                                                                                         >  1:993730/1‑138 (MQ=255)                       aTTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGttt                                                                                                         <  2:993730/138‑1 (MQ=255)                          gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTa                                                                                                    <  1:304336/140‑1 (MQ=255)                          gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTa                                                                                                    <  1:378588/140‑1 (MQ=255)                          gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTa                                                                                                    <  2:344012/140‑1 (MQ=255)                           aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAg                                                                                                   >  1:726352/1‑140 (MQ=255)                           aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGGTTTTGTAg                                                                                                   >  1:779421/1‑140 (MQ=255)                            tttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGa                                                                                                  >  2:288212/1‑140 (MQ=255)                            tttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGa                                                                                                  >  2:1082724/1‑140 (MQ=255)                            tttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGa                                                                                                  >  2:384475/1‑140 (MQ=255)                               gCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATgg                                                                                               <  1:1082724/140‑1 (MQ=255)                               gCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATgg                                                                                               <  2:529064/140‑1 (MQ=255)                                cAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGc                                                                                              >  1:769348/1‑140 (MQ=255)                                        gCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAAGGGTTTTTTTAGATGGCTTTCCAGa                                                                                      >  2:465887/1‑140 (MQ=255)                                         cATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGAc                                                                                     >  1:366487/1‑140 (MQ=255)                                          aTACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACg                                                                                    >  2:380499/1‑140 (MQ=255)                                             cAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTgg                                                                                 <  1:389195/140‑1 (MQ=255)                                                aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCg                                                                              <  2:366487/140‑1 (MQ=255)                                                aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCg                                                                              <  2:769348/140‑1 (MQ=255)                                                 ccAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGt                                                                             <  1:288212/140‑1 (MQ=255)                                                   aGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTc                                                                           >  1:335351/1‑140 (MQ=255)                                                      ttGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAg                                                                        <  2:133810/140‑1 (MQ=255)                                                          ggTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAg                                                                    <  1:384475/140‑1 (MQ=255)                                                                tGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGtt                                                                  >  1:266766/1‑136 (MQ=255)                                                                tGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGtt                                                                  <  2:266766/136‑1 (MQ=255)                                                                tGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGt                                                              <  2:476462/140‑1 (MQ=255)                                                                     tGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGaa                                                         >  1:1020498/1‑140 (MQ=255)                                                                     tGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGaa                                                         >  1:638147/1‑140 (MQ=255)                                                                      ggATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAAt                                                        >  2:142985/1‑140 (MQ=255)                                                                      ggATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAAt                                                        >  2:321057/1‑140 (MQ=255)                                                                          aCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAg                                                    >  2:407651/1‑140 (MQ=255)                                                                            cTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATg                                                       >  2:623939/1‑135 (MQ=255)                                                                            cTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATg                                                       <  1:623939/135‑1 (MQ=255)                                                                            cTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGAt                                                  >  1:1015446/1‑140 (MQ=255)                                                                              tCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTg                                                >  1:871638/1‑140 (MQ=255)                                                                               cATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGa                                               >  2:116839/1‑140 (MQ=255)                                                                                 tatatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATa                                             >  2:12233/1‑140 (MQ=255)                                                                                   tatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGa                                           <  1:321057/140‑1 (MQ=255)                                                                                     tCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAcc                                         >  1:91773/1‑140 (MQ=255)                                                                                      cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAccc                                        >  2:327452/1‑140 (MQ=255)                                                                                      cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAccc                                        >  2:414496/1‑140 (MQ=255)                                                                                      cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAccc                                        >  1:1087132/1‑140 (MQ=255)                                                                                       gATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGa                                           <  1:523678/136‑1 (MQ=255)                                                                                       gATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGa                                           >  2:523678/1‑136 (MQ=255)                                                                                        aTTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAcc                                         <  2:246381/137‑1 (MQ=255)                                                                                        aTTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAcc                                         >  1:246381/1‑137 (MQ=255)                                                                                        aTTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGa                                      >  2:223847/1‑140 (MQ=255)                                                                                             ggAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATtata                                 <  1:358595/140‑1 (MQ=255)                                                                                               aaTACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACt                               >  1:263890/1‑140 (MQ=255)                                                                                                     ggTTTCTTTGCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTAGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCa                         <  1:20995/140‑1 (MQ=255)                                                                                                              gCTGGCGGGCGCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCg                <  1:414496/140‑1 (MQ=255)                                                                                                                  gcgggcgCAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAg            <  2:726352/140‑1 (MQ=255)                                                                                                                       cgcAATCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGTCAGTTGaa       <  2:263890/140‑1 (MQ=255)                                                                                                                            tCAGTCCACGGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgcgc  <  1:223847/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGC  >  NC_000913/2079820‑2080082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑