Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,571	C→T	S62S (TCC→TCT)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,571	0	C	T	100.0%	228.3 / NA	65	S62S (TCC→TCT)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/34);  total (31/34)

CTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCCGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTGATG  >  NC_000913/2158434‑2158707                                                                                                                                          |                                                                                                                                         cTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGt                                                                                                                                        <  2:286665/140‑1 (MQ=255)      ggTTGCCATTACCCTGTGCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCa                                                                                                                                   >  1:1102108/1‑140 (MQ=255)      ggTTGCCATTACCCTGTGCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCa                                                                                                                                   <  2:229742/140‑1 (MQ=255)                     gtgCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCt                                                                                                                    <  2:988547/140‑1 (MQ=255)                     gtgCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCt                                                                                                                    >  1:26404/1‑140 (MQ=255)                      tgCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTc                                                                                                                   >  1:248711/1‑140 (MQ=255)                           cATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTg                                                                                                              <  1:13166/140‑1 (MQ=255)                           cATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTg                                                                                                              >  2:13166/1‑140 (MQ=255)                            aTACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTgg                                                                                                             >  2:443381/1‑140 (MQ=255)                             tACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTggg                                                                                                            <  2:1155096/140‑1 (MQ=255)                               cTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGgcg                                                                                                          <  2:120081/140‑1 (MQ=255)                                tGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGgcgc                                                                                                         >  2:982006/1‑140 (MQ=255)                                tGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGgcgc                                                                                                         >  2:469890/1‑140 (MQ=255)                                tGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGgcgc                                                                                                         >  2:326915/1‑140 (MQ=255)                                tGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGgcgc                                                                                                         >  2:323310/1‑140 (MQ=255)                                       ccGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGc                                                                                                    <  2:410188/138‑1 (MQ=255)                                       ccGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGc                                                                                                    >  1:410188/1‑138 (MQ=255)                                       ccGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCg                                                                                                  <  2:956794/140‑1 (MQ=255)                                          tATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAg                                                                                               >  1:211623/1‑140 (MQ=255)                                          tATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAg                                                                                               <  1:982006/140‑1 (MQ=255)                                              ctgcCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAg                                                                                           <  1:443381/140‑1 (MQ=255)                                                 ccGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGa                                                                                        >  1:399682/1‑140 (MQ=255)                                                 ccGGTCGCCCCGCTGCCGCAGGTCGACTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGa                                                                                        >  2:1017262/1‑140 (MQ=255)                                                  cGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGaa                                                                                       >  2:61729/1‑140 (MQ=255)                                                     tCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATg                                                                                    <  1:675474/140‑1 (MQ=255)                                                     tCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATg                                                                                    <  2:399682/140‑1 (MQ=255)                                                       gCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCTGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGAc                                                                                  <  2:1102108/140‑1 (MQ=255)                                                       gCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGAc                                                                                  <  2:1166969/140‑1 (MQ=255)                                                        ccccGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGAcc                                                                                 >  2:285696/1‑140 (MQ=255)                                                                         gATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCgg                                                                <  1:791048/140‑1 (MQ=255)                                                                             ttCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGgcagc                                                            <  1:285696/140‑1 (MQ=255)                                                                               ccGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCAc                                                          <  2:211623/140‑1 (MQ=255)                                                                                  gTGATTATCATCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCAcgcg                                                       <  1:1043784/140‑1 (MQ=255)                                                                                     aTTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGATCTCCAGCAGTTCGCTCGGCAGCACGCGtat                                                    >  2:776093/1‑140 (MQ=255)                                                                                               gcgcCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAg                                          >  2:673023/1‑140 (MQ=255)                                                                                               gcgcCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAg                                          >  1:777498/1‑140 (MQ=255)                                                                                                cgcCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGt                                         >  2:922433/1‑140 (MQ=255)                                                                                                   cTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTg                                             <  2:1152033/133‑1 (MQ=255)                                                                                                   cTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTg                                             >  1:1152033/1‑133 (MQ=255)                                                                                                   cTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTg                                      >  1:939263/1‑140 (MQ=255)                                                                                                     cGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGAt                                    >  2:406367/1‑140 (MQ=255)                                                                                                      gCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGAtt                                   >  1:1042483/1‑140 (MQ=255)                                                                                                      gCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGAtt                                   >  1:401903/1‑140 (MQ=255)                                                                                                         gCCCGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTg                                <  2:248711/140‑1 (MQ=255)                                                                                                          cccGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGa                               <  1:26335/140‑1 (MQ=255)                                                                                                           ccGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGAc                              <  1:61729/140‑1 (MQ=255)                                                                                                           ccGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGAc                              <  1:406367/140‑1 (MQ=255)                                                                                                            cGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGAcc                             <  2:1013323/140‑1 (MQ=255)                                                                                                            cGGTGCGTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGAcc                             <  1:323310/140‑1 (MQ=255)                                                                                                                  gTCACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGata                       >  1:976431/1‑140 (MQ=255)                                                                                                                    cACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATc                     >  1:668336/1‑140 (MQ=255)                                                                                                                    cACCAGAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATc                     >  1:451499/1‑140 (MQ=255)                                                                                                                         gAAACAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACgg                >  1:773690/1‑140 (MQ=255)                                                                                                                           aaCAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGgcg              <  2:976431/140‑1 (MQ=255)                                                                                                                           aaCAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGgcg              <  2:668336/140‑1 (MQ=255)                                                                                                                            aCAATGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGgcgc             <  2:939263/140‑1 (MQ=255)                                                                                                                               aTGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAgc          <  1:673023/140‑1 (MQ=255)                                                                                                                                tGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGgcg              <  2:577634/135‑1 (MQ=255)                                                                                                                                tGGCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGgcg              >  1:577634/1‑135 (MQ=255)                                                                                                                                  gCGTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGcgcg       <  2:299221/140‑1 (MQ=255)                                                                                                                                    gTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGTAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTg     >  1:357943/1‑140 (MQ=255)                                                                                                                                    gTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTg     <  2:773690/140‑1 (MQ=255)                                                                                                                                    gTCTTCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTg     <  1:922433/140‑1 (MQ=255)                                                                                                                                     tcttctGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAg           >  1:412998/1‑133 (MQ=255)                                                                                                                                     tcttctGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAg           <  2:412998/133‑1 (MQ=255)                                                                                                                                       ttCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTGAtg  <  2:131545/140‑1 (MQ=255)                                                                                                                                       ttCTGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTGAtg  >  1:131545/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGCTTCCGTATGCTGCCGGTCGCCCCGCTGCCGCAGGTCGATTTTCCGGTGATTATCGTCAGCGCCTCGCTGCCCGGTGCGTCACCAGAAACAATGGCGTCTTCCGTTGCCACGCCGCTGGAGCGCTCACTTGGGCGCATTGCCGGAGTCAGTGAAATGACCTCCAGCAGTTCGCTCGGCAGCACGCGTATTATTTTGCAGTTTGATTTTGACCGGGATATCAACGGCGCAGCGCGTGATG  >  NC_000913/2158434‑2158707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑