Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,200,203	G→A	intergenic (+2905/‑76)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,200,203	0	G	A	100.0%	134.4 / NA	42	intergenic (+2905/‑76)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/24);  total (18/24)

TGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAA  >  NC_000913/2200067‑2200337                                                                                                                                         |                                                                                                                                       tGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAAttt                                                                                                                                     >  1:1242370/1‑139 (MQ=255)  ggAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGGAATTCAACACAGGAAGCGCAATtct                                                                                                                                    >  1:1263380/1‑140 (MQ=255)     aaTCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCg                                                                                                                                 <  1:1161131/140‑1 (MQ=255)       tCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTa                                                                                                                               <  1:311282/140‑1 (MQ=255)       tCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAACGCAATTCTCCGTa                                                                                                                               >  2:1196577/1‑140 (MQ=255)             aaGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCCGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGAt                                                                                                                         <  1:1195832/140‑1 (MQ=255)                gCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGcc                                                                                                                      <  1:537505/140‑1 (MQ=255)                 cTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCa                                                                                                                     <  2:1138785/140‑1 (MQ=255)                    tGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCa                                                                                                                  >  2:755332/1‑140 (MQ=255)                               tGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAg                                                                                                       <  1:641240/140‑1 (MQ=255)                                 tCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGTGGAAGTCAACACAGGAAGCGCAAATCTCCGTAATCGAGTGCATCACCGCCAGGGAGCt                                                                                                     >  2:749415/1‑140 (MQ=255)                                   gCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGCAATCGATGCCATCACCGCCAGCGAGCTaa                                                                                                   >  2:172581/1‑140 (MQ=255)                                     cAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAAtt                                                                                                 >  2:825814/1‑140 (MQ=255)                                       gCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTaa                                                                                               <  2:1263380/140‑1 (MQ=255)                                          gAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGa                                                                                            <  1:781285/140‑1 (MQ=255)                                          gAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGa                                                                                            <  1:749415/140‑1 (MQ=255)                                           aaCTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTTGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGat                                                                                           >  1:375081/1‑140 (MQ=255)                                           aaCTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGat                                                                                           >  2:368929/1‑140 (MQ=255)                                               cAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTg                                                                                       <  1:755332/140‑1 (MQ=255)                                                  cgcTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAg                                                                                    >  2:198791/1‑140 (MQ=255)                                                   gcTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGc                                                                                   <  2:602399/140‑1 (MQ=255)                                                   gcTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGc                                                                                   <  1:278580/140‑1 (MQ=255)                                                    cTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGcc                                                                                  <  1:825814/140‑1 (MQ=255)                                                       agcagcTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTg                                                                               <  1:272839/140‑1 (MQ=255)                                                             tctTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGaa                                                                         <  1:198791/140‑1 (MQ=255)                                                               ttAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATa                                                                       <  1:407639/140‑1 (MQ=255)                                                                   gcaagcTGTTGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGa                                                                   <  2:375081/140‑1 (MQ=255)                                                                        cTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAagag                                                              >  1:29490/1‑140 (MQ=255)                                                                        cTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAagag                                                              >  1:1221335/1‑140 (MQ=255)                                                                             gCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATgg                                                         >  2:1227132/1‑140 (MQ=255)                                                                              ttATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGa                                                        <  2:961671/139‑1 (MQ=255)                                                                                       ggCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAAtt                                               >  1:537336/1‑140 (MQ=255)                                                                                       ggCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAAtt                                               >  1:537119/1‑140 (MQ=255)                                                                                       ggCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAAtt                                               >  2:839568/1‑140 (MQ=255)                                                                                                  aaGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtggctgg                                    <  2:29490/140‑1 (MQ=255)                                                                                                  aaGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtggctgg                                    <  1:839568/140‑1 (MQ=255)                                                                                                               gggAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtgg                                        >  2:1082567/1‑123 (MQ=255)                                                                                                               gggAGTAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtgg                                        <  1:1082567/123‑1 (MQ=255)                                                                                                                    tAATTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTgg                  >  1:453844/1‑140 (MQ=255)                                                                                                                      aTTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGaa                <  2:341696/140‑1 (MQ=255)                                                                                                                      aTTCAACACAGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGaa                <  1:172581/140‑1 (MQ=255)                                                                                                                             acaGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAat         <  2:537336/140‑1 (MQ=255)                                                                                                                              caGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAata        >  1:1084641/1‑140 (MQ=255)                                                                                                                               aGGAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAatat       >  2:494045/1‑140 (MQ=255)                                                                                                                                 gAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATaa     >  1:924313/1‑140 (MQ=255)                                                                                                                                 gAAGCGCAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATaa     >  2:73565/1‑140 (MQ=255)                                                                                                                                    gcgcAATTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATaaaaa  >  2:579572/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGCAGCTCTTAAGCAAGCTGTGGCTATGGGAAGGCAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAA  >  NC_000913/2200067‑2200337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑