Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,876	C→T	L57F (CTC→TTC)	yfcH →	putative NAD‑dependent epimerase YfcH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,876	0	C	T	100.0%	235.9 / NA	67	L57F (CTC→TTC)	yfcH	putative NAD‑dependent epimerase YfcH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/32);  total (35/32)

AATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCC  >  NC_000913/2421740‑2422010                                                                                                                                         |                                                                                                                                       aaTTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCa                                                                                                                                     <  2:811157/140‑1 (MQ=255)  aTTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCaa                                                                                                                                    >  2:88190/1‑140 (MQ=255)  aTTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCaa                                                                                                                                    <  2:344218/140‑1 (MQ=255)       tCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCg                                                                                                                               >  1:1023442/1‑140 (MQ=255)       tCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCg                                                                                                                               >  1:875503/1‑140 (MQ=255)       tCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCg                                                                                                                               >  1:571720/1‑140 (MQ=255)        cGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGt                                                                                                                              >  2:1162389/1‑140 (MQ=255)             tttGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATg                                                                                                                         <  2:896686/140‑1 (MQ=255)                gATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCgg                                                                                                                      <  1:1092290/140‑1 (MQ=255)                   tCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTaa                                                                                                                   <  1:844007/140‑1 (MQ=255)                     cgcgTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATc                                                                                                                 >  2:1047883/1‑140 (MQ=255)                     cgcgTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATc                                                                                                                 >  2:318452/1‑140 (MQ=255)                     cgcgTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATc                                                                                                                 >  1:716992/1‑140 (MQ=255)                       cgTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCaa                                                                                                               >  2:617846/1‑140 (MQ=255)                            gCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTgg                                                                                                          <  1:617846/140‑1 (MQ=255)                                 aGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGga                                                                                                     <  1:625993/140‑1 (MQ=255)                                 aGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGga                                                                                                     >  2:867948/1‑140 (MQ=255)                                 aGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCCGGCCGga                                                                                                     <  2:571720/140‑1 (MQ=255)                                           tCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTg                                                                                           >  1:335155/1‑140 (MQ=255)                                           tCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTg                                                                                           >  1:392712/1‑140 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      <  1:567506/140‑1 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      <  1:1097558/140‑1 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      <  1:376407/140‑1 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      <  2:498715/140‑1 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      <  2:1124055/140‑1 (MQ=255)                                                ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGAt                                                                                      >  2:749543/1‑140 (MQ=255)                                                         tGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTgg                                                                             >  1:974224/1‑140 (MQ=255)                                                         tGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTgg                                                                             >  1:992909/1‑140 (MQ=255)                                                            cgcgTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACt                                                                          <  2:392712/140‑1 (MQ=255)                                                              cgTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAGGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCa                                                                        >  2:564943/1‑140 (MQ=255)                                                                tAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACg                                                                      >  1:765598/1‑140 (MQ=255)                                                                tAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACg                                                                      >  2:510355/1‑140 (MQ=255)                                                                tAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACg                                                                      >  1:1268636/1‑140 (MQ=255)                                                                  aCCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAg                                                                    <  1:1261525/140‑1 (MQ=255)                                                                  aCCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAg                                                                    >  2:1261525/1‑140 (MQ=255)                                                                   cccGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGc                                                                   >  1:394845/1‑140 (MQ=255)                                                                      gCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCaaa                                                                <  1:867948/140‑1 (MQ=255)                                                                       cAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCaaaa                                                               >  1:616476/1‑140 (MQ=255)                                                                          aaaGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAaga                                                            <  1:749543/140‑1 (MQ=255)                                                                                       ttCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCaa                                               <  1:1047883/140‑1 (MQ=255)                                                                                        tctcGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCaaa                                              >  1:952915/1‑140 (MQ=255)                                                                                          tcGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAGGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGc                                            <  1:564943/140‑1 (MQ=255)                                                                                          tcGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGc                                            <  1:88190/140‑1 (MQ=255)                                                                                              cccTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCt                                        >  1:779758/1‑140 (MQ=255)                                                                                              cccTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAACAAAAAGAGCGTCTCTGCCAAAGCCGCt                                        <  2:765598/140‑1 (MQ=255)                                                                                                 tCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCCGGa                                     <  1:437379/140‑1 (MQ=255)                                                                                                     gTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAGCCTAGCCGGAGAGCTGATTGCTAGTAAACGCTGGACGAACGAGCAAAAAGAGCGACTCTGCCAAAGCCGCTGGAatat                                 <  2:716992/140‑1 (MQ=255)                                                                                                        acacTATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc                              >  1:767762/1‑140 (MQ=255)                                                                                                            tATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaa                          >  1:1134995/1‑140 (MQ=255)                                                                                                            tATGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaa                          >  2:597000/1‑140 (MQ=255)                                                                                                             aTGGCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaaa                         >  1:726632/1‑140 (MQ=255)                                                                                                               ggCAAGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaaaaa                       >  2:1180070/1‑140 (MQ=255)                                                                                                                   aGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGCTTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc                              <  2:865336/129‑1 (MQ=255)                                                                                                                   aGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc                              >  1:865336/1‑129 (MQ=255)                                                                                                                   aGGGCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTgg                   <  1:597000/140‑1 (MQ=255)                                                                                                                     ggCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGt                  >  2:476002/1‑139 (MQ=255)                                                                                                                     ggCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGt                  <  1:476002/139‑1 (MQ=255)                                                                                                                      gCTTGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCg                >  1:768702/1‑140 (MQ=255)                                                                                                                        ttGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGAt              <  1:318452/140‑1 (MQ=255)                                                                                                                        ttGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGAt              >  2:1025016/1‑140 (MQ=255)                                                                                                                         tGCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGAtt             <  2:974224/140‑1 (MQ=255)                                                                                                                          gCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTgg                   <  2:773424/133‑1 (MQ=255)                                                                                                                          gCCGATCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTgg                   >  1:773424/1‑133 (MQ=255)                                                                                                                              aTCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGAtt        <  2:768702/140‑1 (MQ=255)                                                                                                                               tCAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTa       <  2:779758/140‑1 (MQ=255)                                                                                                                                cAAAGCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTaa      >  1:936218/1‑140 (MQ=255)                                                                                                                                    gCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGcc  <  1:351136/140‑1 (MQ=255)                                                                                                                                    gCAACTTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGcc  <  2:273838/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCC  >  NC_000913/2421740‑2422010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑