Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,455,599	G→A	G16G (GGC→GGT)	yfcV ←	putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,455,599	0	G	A	100.0%	245.9 / NA	71	G16G (GGC→GGT)	yfcV	putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/38);  total (33/38)

CGGCACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCGCCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTG  >  NC_000913/2455463‑2455733                                                                                                                                         |                                                                                                                                       cGGCACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCAccc                                                                                                                                     <  1:727942/140‑1 (MQ=255) cGGCACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCAccc                                                                                                                                     <  1:411211/140‑1 (MQ=255)   gCACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCAt                                                                                                                                   <  1:672893/140‑1 (MQ=255)    cACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATc                                                                                                                                  <  1:614108/140‑1 (MQ=255)    cACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATc                                                                                                                                  <  2:410192/140‑1 (MQ=255)      ccGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCAc                                                                                                                                >  1:760738/1‑140 (MQ=255)      ccGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCAc                                                                                                                                >  1:409437/1‑140 (MQ=255)         atatCACTCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCAt                                                                                                                             <  1:82904/140‑1 (MQ=255)                 cATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCgcag                                                                                                                     >  2:626739/1‑140 (MQ=255)                 cATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCgcag                                                                                                                     >  2:1235164/1‑140 (MQ=255)                  atatCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCgcagc                                                                                                                    <  2:220813/140‑1 (MQ=255)                   tatCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCgcagca                                                                                                                   >  1:1091109/1‑140 (MQ=255)                       aaCTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAg                                                                                                               >  2:393861/1‑140 (MQ=255)                          ttCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTg                                                                                                            >  2:609281/1‑140 (MQ=255)                          ttCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTg                                                                                                            <  1:1235164/140‑1 (MQ=255)                             cAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGttt                                                                                                         >  2:5159/1‑140 (MQ=255)                              aGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGtttt                                                                                                        <  1:1065422/140‑1 (MQ=255)                                    tgATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACaaa                                                                                                  <  2:623274/140‑1 (MQ=255)                                        cGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTa                                                                                              <  1:221274/140‑1 (MQ=255)                                        cGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTa                                                                                              <  2:409437/140‑1 (MQ=255)                                        cGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTa                                                                                              <  2:760738/140‑1 (MQ=255)                                          tCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACt                                                                                            <  1:155499/140‑1 (MQ=255)                                          tCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACt                                                                                            >  1:986505/1‑140 (MQ=255)                                              gAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCatt                                                                                        >  1:860222/1‑140 (MQ=255)                                                 cGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCattatt                                                                                     <  2:986505/140‑1 (MQ=255)                                                 cGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCattatt                                                                                     <  2:1055278/140‑1 (MQ=255)                                                 cGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCattatt                                                                                     <  2:780521/140‑1 (MQ=255)                                                   aTAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTg                                                                                   >  2:1025861/1‑140 (MQ=255)                                                         cacacTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTa                                                                             >  1:220422/1‑140 (MQ=255)                                                            acTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAAc                                                                          <  2:673252/140‑1 (MQ=255)                                                                aGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCtt                                                                              <  2:189225/132‑1 (MQ=255)                                                                aGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCtt                                                                              >  1:189225/1‑132 (MQ=255)                                                                aGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACtata                                                                      <  1:669291/140‑1 (MQ=255)                                                                 gTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTataa                                                                     <  1:609281/140‑1 (MQ=255)                                                                       aaTGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAAtt                                                               <  1:393861/140‑1 (MQ=255)                                                                         tGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTaa                                                             <  2:1091109/140‑1 (MQ=255)                                                                            tGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTataaa                                                                    <  2:820744/130‑1 (MQ=255)                                                                            tGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTataaa                                                                    >  1:820744/1‑130 (MQ=255)                                                                            tGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAat                                                          >  1:1163586/1‑140 (MQ=255)                                                                            tGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAat                                                          >  2:1070680/1‑140 (MQ=255)                                                                               cGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAAtata                                                       <  1:5159/140‑1 (MQ=255)                                                                               cGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAAtata                                                       <  2:399220/140‑1 (MQ=255)                                                                                   aaaaCGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACa                                                   <  2:860222/140‑1 (MQ=255)                                                                                   aaaaCGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACa                                                   <  2:220422/140‑1 (MQ=255)                                                                                    aaaCGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACAt                                                  <  2:963375/140‑1 (MQ=255)                                                                                        gTGCAGTACCGTTGTTACGCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCa                                              >  2:947607/1‑140 (MQ=255)                                                                                         tGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCaa                                             <  1:1025861/140‑1 (MQ=255)                                                                                          gCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAAt                                            >  1:572223/1‑140 (MQ=255)                                                                                             gTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACa                                         <  1:1169585/140‑1 (MQ=255)                                                                                                   ttgttACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGAtt                                   >  2:808126/1‑140 (MQ=255)                                                                                                   ttgttACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGAtt                                   >  1:1160484/1‑140 (MQ=255)                                                                                                   ttgttACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGAtt                                   >  1:488922/1‑140 (MQ=255)                                                                                                    tgttACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTc                                  <  1:244105/140‑1 (MQ=255)                                                                                                     gttACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCa                                 <  1:626739/140‑1 (MQ=255)                                                                                                        aCCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAGTAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTc                              >  2:148572/1‑140 (MQ=255)                                                                                                        aCCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTc                              >  1:166518/1‑140 (MQ=255)                                                                                                        aCCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTc                              >  1:163343/1‑140 (MQ=255)                                                                                                        aCCCGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTc                              >  2:431068/1‑140 (MQ=255)                                                                                                          ccGCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCaa                            >  2:767959/1‑140 (MQ=255)                                                                                                            gCAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATa                          >  1:894626/1‑140 (MQ=255)                                                                                                             cAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTc                                  >  1:955558/1‑131 (MQ=255)                                                                                                             cAGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTc                                  <  2:955558/131‑1 (MQ=255)                                                                                                              aGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCGATAGc                        >  1:1095032/1‑140 (MQ=255)                                                                                                              aGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGc                        >  1:833592/1‑140 (MQ=255)                                                                                                              aGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGc                        >  1:768997/1‑140 (MQ=255)                                                                                                              aGCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGc                        >  1:192228/1‑140 (MQ=255)                                                                                                               gCGATTGTTGCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGcc                       <  1:431068/140‑1 (MQ=255)                                                                                                                cgcttgttgCCGTAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCGATAGCCa                      <  2:1095032/137‑1 (MQ=255)                                                                                                                           gTAGAGGTGAACGCACCCATCGCCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATGcc           <  1:569100/140‑1 (MQ=255)                                                                                                                            tAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATAccc          <  2:768997/140‑1 (MQ=255)                                                                                                                            tAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATAccc          >  2:837972/1‑140 (MQ=255)                                                                                                                            tAGAGGTGAACGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATAccc          <  2:192228/140‑1 (MQ=255)                                                                                                                                    aaCGCACCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTg  >  2:502595/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGGCACCGATATCACCCATATCAACTTCCAGTTTGTGATCGTCCGGAACGATAGAACACACTGAGTCTTCAATGGTGCCGTAAAAACGTGCAGTACCGTTGTTACCCGCAGCGATTGTTGCCGTAGAGGTGAACGCGCCCATCACCATTGCCGCAGCAATAGCTGTTTTAACAAACTTACTCATTATTTGTCCTTAAACTATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTG  >  NC_000913/2455463‑2455733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑