Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,759,677	C→T	V231V (GTC→GTT)	yfjI →	CP4‑57 prophage; protein YfjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,759,677	0	C	T	100.0%	254.6 / NA	74	V231V (GTC→GTT)	yfjI	CP4‑57 prophage; protein YfjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/37);  total (37/37)

ATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTCCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTC  >  NC_000913/2759540‑2759811                                                                                                                                          |                                                                                                                                       aTCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCa                                                                                                                                      <  2:914272/140‑1 (MQ=255)     tttttAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCt                                                                                                                                  >  2:369011/1‑140 (MQ=255)      ttttAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTa                                                                                                                                 <  1:244855/140‑1 (MQ=255)         tAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGcc                                                                                                                                   >  2:833249/1‑135 (MQ=255)         tAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGcc                                                                                                                                   <  1:833249/135‑1 (MQ=255)         tAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATg                                                                                                                              <  1:971857/140‑1 (MQ=255)         tAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATg                                                                                                                              <  2:1266459/140‑1 (MQ=255)              gTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGttttt                                                                                                                         <  1:787440/140‑1 (MQ=255)                tACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTaa                                                                                                                       <  2:683734/140‑1 (MQ=255)                       ttAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTtat                                                                                                                >  1:1019711/1‑140 (MQ=255)                        tAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTtata                                                                                                               >  2:586063/1‑140 (MQ=255)                        tAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTtata                                                                                                               >  1:79837/1‑140 (MQ=255)                        tAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTtata                                                                                                               >  2:1184144/1‑140 (MQ=255)                         aaCGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTatat                                                                                                              >  1:1091587/1‑140 (MQ=255)                         aaCGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTatat                                                                                                              >  1:347336/1‑140 (MQ=255)                           cGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCg                                                                                                            >  1:461614/1‑140 (MQ=255)                           cGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCg                                                                                                            >  1:189750/1‑140 (MQ=255)                            gAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGa                                                                                                           <  2:1091587/140‑1 (MQ=255)                            gAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGa                                                                                                           <  2:728062/140‑1 (MQ=255)                             aGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGAc                                                                                                          <  2:454167/140‑1 (MQ=255)                               cTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAg                                                                                                        >  1:851736/1‑140 (MQ=255)                               cTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAg                                                                                                        >  1:471611/1‑140 (MQ=255)                               cTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAg                                                                                                        >  2:712880/1‑140 (MQ=255)                                tGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAgg                                                                                                       <  1:904068/140‑1 (MQ=255)                                tGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAgg                                                                                                       <  2:98329/140‑1 (MQ=255)                                 gCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGa                                                                                                      >  1:1220574/1‑140 (MQ=255)                                 gCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGa                                                                                                      >  1:1112196/1‑140 (MQ=255)                                   cGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGaaa                                                                                                    <  1:36840/140‑1 (MQ=255)                                     tttATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAgg                                                                                                  <  1:586063/140‑1 (MQ=255)                                     tttATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAgg                                                                                                  <  1:1184144/140‑1 (MQ=255)                                        aTCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGgaga                                                                                               <  2:79837/140‑1 (MQ=255)                                          cAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGata                                                                                             <  2:1220574/140‑1 (MQ=255)                                          cAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGata                                                                                             <  2:1112196/140‑1 (MQ=255)                                            aTAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGTAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATg                                                                                           >  2:287901/1‑140 (MQ=255)                                                 aTGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCaaa                                                                                      <  1:410605/140‑1 (MQ=255)                                                     gggATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAgggg                                                                                  >  1:1165652/1‑140 (MQ=255)                                                          ggTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGggattgg                                                                             >  1:684318/1‑140 (MQ=255)                                                           gTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGggattgga                                                                            >  2:393413/1‑140 (MQ=255)                                                                    ttACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCa                                                                   <  2:189750/140‑1 (MQ=255)                                                                    ttACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCa                                                                   <  2:461614/140‑1 (MQ=255)                                                                     tACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCAc                                                                  <  2:1165652/140‑1 (MQ=255)                                                                     tACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCAc                                                                  >  1:618133/1‑140 (MQ=255)                                                                      aCGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACg                                                                 <  2:1019711/140‑1 (MQ=255)                                                                        ggTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGt                                                               <  1:516493/140‑1 (MQ=255)                                                                        ggTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGt                                                               <  2:296144/140‑1 (MQ=255)                                                                         gTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTg                                                              <  1:1220623/140‑1 (MQ=255)                                                                           ggaaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGcc                                                            >  2:86902/1‑140 (MQ=255)                                                                             aaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGc                                                                    <  2:518802/130‑1 (MQ=255)                                                                             aaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGc                                                                    >  1:518802/1‑130 (MQ=255)                                                                             aaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGt                                                               >  2:695869/1‑135 (MQ=255)                                                                             aaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGt                                                               <  1:695869/135‑1 (MQ=255)                                                                             aaaggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGATGCCTc                                                          >  1:704005/1‑140 (MQ=255)                                                                               aggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATTGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCAc                                                                  <  1:1067266/130‑1 (MQ=255)                                                                               aggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCAc                                                                  >  2:1067266/1‑130 (MQ=255)                                                                               aggaaaAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCAt                                                        >  1:1126623/1‑140 (MQ=255)                                                                                   aaaaCGAGCCCGAGAAACTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGc                                                    <  2:1126623/140‑1 (MQ=255)                                                                                        gAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGcc                                               <  2:471611/140‑1 (MQ=255)                                                                                            ccGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTc                                                          <  1:1262846/125‑1 (MQ=255)                                                                                            ccGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTc                                                          >  2:1262846/1‑125 (MQ=255)                                                                                              gagaAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTc                                         <  2:347336/140‑1 (MQ=255)                                                                                                gaAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCaa                                       <  1:395490/140‑1 (MQ=255)                                                                                                  aattaattAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAaca                                     >  2:792651/1‑140 (MQ=255)                                                                                                  aattaattAGAGATGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAaca                                     <  2:684318/140‑1 (MQ=255)                                                                                                            agaTGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACa                           <  1:86902/140‑1 (MQ=255)                                                                                                              aTGCAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGa                         >  2:500487/1‑140 (MQ=255)                                                                                                                 cAAGAATAACACTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGaaaa                      <  1:393413/140‑1 (MQ=255)                                                                                                                          cacTGTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAAc             >  1:585519/1‑140 (MQ=255)                                                                                                                              gTCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCaa         >  1:827492/1‑140 (MQ=255)                                                                                                                               tCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAAt        >  2:1060333/1‑140 (MQ=255)                                                                                                                               tCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAAt        >  1:421000/1‑140 (MQ=255)                                                                                                                               tCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAAt        >  2:483340/1‑140 (MQ=255)                                                                                                                               tCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAAt        >  1:561014/1‑140 (MQ=255)                                                                                                                               tCGCTGATGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTACAACCCAAt        >  1:1131619/1‑140 (MQ=255)                                                                                                                                     aTGGTTCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTc  >  1:160772/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATTAGAGATGCAAGAATAACACTGTCGCTGATGGTCCAGCCTAATGTTTTTAAGGGTTATATCGACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTC  >  NC_000913/2759540‑2759811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑